Restriction map for
pF1KB8184
##################################
# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaHI BsaI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
ClaI CpoI DpnII DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeII MaeIII MamI MboI MfeI
MflI MluI MroI MseI MunI
NarI NdeI NdeII NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 874 AAATTC [GA]^AATT[CT]
AfaI 726 GTAC GT^AC
AlwNI 326 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 874 AAATTC [GA]^AATT[CT]
AvaI 166 CCCGAG C^[CT]CG[GA]G
AviII 495 TGCGCA TGC^GCA
BanI 592 GGTGCC G^G[CT][GA]CC
BanII 199 GAGCCC G[GA]GC[CT]^C
BcgI 552 CGAGGCGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 672 CCCGG CC^[GC]GG
BlpI 432 GCTGAGC GC^T[ACGT]AGC
BmyI 199 GAGCCC G[AGT]GC[ACT]^C
BpmI 919 CTGGAG CTGGAG(16/14)
Bpu1102I 432 GCTGAGC GC^T[ACGT]AGC
BsaWI 953 TCCGGT [AT]^CCGG[AT]
BsmFI 471 GGGAC GGGAC(10/14)
BsoBI 166 CCCGAG C^[CT]CG[GA]G
Bsp1286I 199 GAGCCC G[AGT]GC[ACT]^C
BstF5I 27 GGATG GGATG(2/0)
CelII 432 GCTGAGC GC^T[ACGT]AGC
EcoT14I 371 CCATGG C^C[AT][AT]GG
FokI 27 GGATG GGATG(9/13)
FspI 495 TGCGCA TGC^GCA
HaeII 634 GGCGCT [GA]GCGC^[CT]
HphI 927 GGTGA GGTGA(8/7)
NciI 672 CCCGG CC^[GC]GG
NcoI 371 CCATGG C^CATGG
NspI 791 GCATGT [GA]CATG^[CT]
PleI 298 GAGTC GAGTC(4/5)
PvuII 475 CAGCTG CAG^CTG
RsaI 726 GTAC GT^AC
StuI 654 AGGCCT AGG^CCT
StyI 371 CCATGG C^C[AT][AT]GG
TfiI 846 GAATC G^A[AT]TC
Tsp509I 875 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BspMI 589 GCAGGT ACCTGC(4/8)
676 GCAGGT
BsrFI 31 GCCGGC [GA]^CCGG[CT]
806 GCCGGC
BsrI 856 ACTGG ACTGG(1/-1)
612 CCAGT
BssHII 698 GCGCGC G^CGCGC
797 GCGCGC
BstAPI 325 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
815 GCATCAACTGC
BstXI 371 CCATGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
452 CCACGGAGCTGG
Cfr10I 31 GCCGGC [GA]^CCGG[CT]
806 GCCGGC
DsaI 371 CCATGG C^C[GA][CT]GG
452 CCACGG
EarI 246 CTCTTC CTCTTC(1/4)
658 CTCTTC
HgaI 480 GACGC GACGC(5/10)
171 GCGTC
HinfI 298 GAGTC G^A[ACGT]TC
846 GAATC
Ksp632I 246 CTCTTC CTCTTC(1/4)
658 CTCTTC
NaeI 31 GCCGGC GCC^GGC
806 GCCGGC
NgoMIV 31 GCCGGC G^CCGGC
806 GCCGGC
PstI 323 CTGCAG CTGCA^G
484 CTGCAG
SfcI 323 CTGCAG C^T[GA][CT]AG
484 CTGCAG
TaqI 4 TCGA T^CGA
849 TCGA
TthHB8I 4 TCGA T^CGA
849 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AluI BalI BbvI
BglI BsaJI BsgI BsiYI BslI
BsrBI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI EaeI EcoRII
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HpaII ItaI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NlaIII NlaIV
Sau96I ScrFI SfaNI TseI TspRI