Restriction map for
pF1KB6452
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I Aor51HI ApaLI
AscI Asp700I Asp718I AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspMI BsrBI BsrFI BsrGI
BssHII Bst1107I BstEII BstPI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EarI Eco47III
EcoNI EcoO65I EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I MaeIII MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI PvuII RsrII SalI
SapI SbfI SexAI SfaNI SfcI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp45I Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 34 GTCTAC GT^[AC][GT]AC
AcsI 541 GAATTC [GA]^AATT[CT]
AflIII 644 ACATGT A^C[GA][CT]GT
AocI 886 CCTGAGG CC^T[ACGT]AGG
ApaI 26 GGGCCC GGGCC^C
ApoI 541 GAATTC [GA]^AATT[CT]
AseI 172 ATTAAT AT^TAAT
AsnI 172 ATTAAT AT^TAAT
BglI 946 GCCAAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 897 AGATCT A^GATCT
BpmI 256 CTGGAG CTGGAG(16/14)
BsaI 1033 GGTCTC GGTCTC(1/5)
BsaWI 401 TCCGGT [AT]^CCGG[AT]
BsgI 1007 GTGCAG GTGCAG(16/14)
BsiEI 11 CGGCCG CG[GA][CT]^CG
BspHI 488 TCATGA T^CATGA
BspLU11I 644 ACATGT A^CATGT
BsrDI 735 CATTGC GCAATG(2/0)
BssSI 610 CTCGTG CACGAG(-5/-1)
BstAPI 1009 GCAGACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 819 CCTGG CC^[AT]GG
BstXI 740 CCAAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 886 CCTGAGG CC^T[ACGT]AGG
EaeI 11 CGGCCG [CT]^GGCC[GA]
EagI 11 CGGCCG C^GGCCG
EclXI 11 CGGCCG C^GGCCG
Eco52I 11 CGGCCG C^GGCCG
Eco81I 886 CCTGAGG CC^T[ACGT]AGG
EcoRI 541 GAATTC G^AATTC
EcoRII 819 CCTGG ^CC[AT]GG
FauI 16 GCGGG CCCGC(4/6)
KspI 14 CCGCGG CCGC^GG
MaeII 89 ACGT A^CGT
MslI 463 CACAGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 14 CCGCGG C[AC]G^C[GT]G
MvaI 819 CCTGG CC^[AT]GG
NspI 644 ACATGT [GA]CATG^[CT]
PpuMI 308 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 172 ATTAAT AT^TAAT
RcaI 488 TCATGA T^CATGA
SacI 604 GAGCTC GAGCT^C
SacII 14 CCGCGG CCGC^GG
ScaI 68 AGTACT AGT^ACT
SmaI 29 CCCGGG CCC^GGG
SmlI 582 CTCAAG C^T[CT][GA]AG
Tsp509I 542 AATT ^AATT
XcmI 947 CCAAAGAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 29 CCCGGG C^CCGGG
XmaI 29 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 15 CGCG CG^CG
64 CGCG
AhdI 310 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
872 GACACCTTGTC
AlwI 381 GATCC GGATC(4/5)
799 GATCC
AlwNI 379 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
934 CAGAGACTG
AspEI 310 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
872 GACACCTTGTC
AspHI 604 GAGCTC G[AT]GC[AT]^C
961 GAGCAC
AvaI 29 CCCGGG C^[CT]CG[GA]G
93 CCCGAG
AvaII 309 GGACC G^G[AT]CC
598 GGACC
BanII 26 GGGCCC G[GA]GC[CT]^C
604 GAGCTC
BfaI 796 CTAG C^TAG
895 CTAG
BseRI 607 CTCCTC GAGGAG(10/8)
57 GAGGAG
BsiHKAI 604 GAGCTC G[AT]GC[AT]^C
961 GAGCAC
BsmAI 936 GAGAC GTCTC(1/5)
1034 GTCTC
BsoBI 29 CCCGGG C^[CT]CG[GA]G
93 CCCGAG
BstBI 128 TTCGAA TT^CGAA
683 TTCGAA
BstF5I 550 CATCC GGATG(2/0)
468 GGATG
BstUI 15 CGCG CG^CG
64 CGCG
Cac8I 16 GCGGGC GC[ACGT]^[ACGT]GC
670 GCAAGC
Eam1105I 310 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
872 GACACCTTGTC
EcoRV 397 GATATC GAT^ATC
814 GATATC
FokI 550 CATCC GGATG(9/13)
468 GGATG
HinfI 252 GATTC G^A[ACGT]TC
455 GAATC
MaeI 796 CTAG C^TAG
895 CTAG
MvnI 15 CGCG CG^CG
64 CGCG
NcoI 702 CCATGG C^CATGG
956 CCATGG
NspV 128 TTCGAA TT^CGAA
683 TTCGAA
SfuI 128 TTCGAA TT^CGAA
683 TTCGAA
TaqI 129 TCGA T^CGA
684 TCGA
TfiI 252 GATTC G^A[AT]TC
455 GAATC
TthHB8I 129 TCGA T^CGA
684 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI BbvI BcnI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstYI Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoT14I Fnu4HI HaeIII HapII HpaII
HphI ItaI MboI MboII MflI
MnlI MseI MspI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI StyI Tru9I TseI
TspRI XhoII