Restriction map for
pF1KB6390
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaII
AviII AvrII BalI BamHI BbrPI
BbvCI BciVI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsgI BsiWI BsmAI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DdeI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HinfI HpaI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MslI MunI NaeI
NdeI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TfiI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
###################################
BbsI 879 GAAGAC GAAGAC(2/6)
BcgI 82 CGACGCGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 590 TGATCA T^GATCA
BglII 561 AGATCT A^GATCT
BpuAI 879 GAAGAC GAAGAC(2/6)
BssHII 19 GCGCGC G^CGCGC
BssSI 27 CTCGTG CACGAG(-5/-1)
BstF5I 447 GGATG GGATG(2/0)
BstYI 561 AGATCT [GA]^GATC[CT]
DsaI 316 CCATGG C^C[GA][CT]GG
EarI 647 CTCTTC CTCTTC(1/4)
EcoT14I 316 CCATGG C^C[AT][AT]GG
FbaI 590 TGATCA T^GATCA
FokI 447 GGATG GGATG(9/13)
Ksp632I 647 CTCTTC CTCTTC(1/4)
MaeIII 860 GTTAC ^GT[ACGT]AC
MflI 561 AGATCT [GA]^GATC[CT]
MnlI 147 GAGG CCTC(7/6)
NcoI 316 CCATGG C^CATGG
NheI 3 GCTAGC G^CTAGC
PvuII 842 CAGCTG CAG^CTG
SfaNI 847 GCATC GCATC(5/9)
SfiI 274 GGCCAGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 400 AATATT AAT^ATT
StyI 316 CCATGG C^C[AT][AT]GG
Tsp509I 351 AATT ^AATT
XhoII 561 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 46 GGCGCC G[GA]^CG[CT]C
250 GGCGCC
AlwI 505 GATCC GGATC(4/5)
185 GGATC
AspHI 291 GAGCAC G[AT]GC[AT]^C
497 GTGCTC
AvaI 181 CCCGGG C^[CT]CG[GA]G
267 CCCGGG
BanI 46 GGCGCC G^G[CT][GA]CC
250 GGCGCC
BbeI 46 GGCGCC GGCGC^C
250 GGCGCC
BpmI 776 CTCCAG CTGGAG(16/14)
144 CTGGAG
BsaHI 46 GGCGCC G[GA]^CG[CT]C
250 GGCGCC
BsiHKAI 291 GAGCAC G[AT]GC[AT]^C
497 GTGCTC
BsoBI 181 CCCGGG C^[CT]CG[GA]G
267 CCCGGG
BsrBI 161 CCGCTC CCGCTC(-3/-3)
245 GAGCGG
BstNI 132 CCTGG CC^[AT]GG
230 CCAGG
EcoRII 132 CCTGG ^CC[AT]GG
230 CCAGG
FauI 214 GCGGG CCCGC(4/6)
247 GCGGG
HaeII 46 GGCGCC [GA]GCGC^[CT]
250 GGCGCC
HgaI 83 GACGC GACGC(5/10)
153 GCGTC
Hin1I 46 GGCGCC G[GA]^CG[CT]C
250 GGCGCC
KasI 46 GGCGCC G^GCGCC
250 GGCGCC
MvaI 132 CCTGG CC^[AT]GG
230 CCAGG
NarI 46 GGCGCC GG^CGCC
250 GGCGCC
NlaIII 317 CATG CATG^
588 CATG
SfoI 46 GGCGCC GGC^GCC
250 GGCGCC
SmaI 181 CCCGGG CCC^GGG
267 CCCGGG
TaqI 462 TCGA T^CGA
598 TCGA
TthHB8I 462 TCGA T^CGA
598 TCGA
XmaCI 181 CCCGGG C^CCGGG
267 CCCGGG
XmaI 181 CCCGGG C^CCGGG
267 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AluI ApaI BanII
BbvI BcnI BfaI BglI BmyI
BsaJI BsiEI BsiYI BslI Bsp1286I
BsrI BssKI BstUI Cac8I CfoI
Cfr13I DpnII DraII EaeI EagI
EclXI Eco52I EcoO109I Fnu4HI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeI MboI MboII MseI
MspA1I MspI MvnI MwoI NciI
NdeII NlaIV Sau3AI Sau96I ScrFI
Tru9I TseI TspRI