Restriction map for
pF1KB7801
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AccII AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BbsI BcgI BciVI
BclI BfrI BglI BglII BlnI
BpuAI BsaAI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HgaI HincII
HindII HpaI KspI MfeI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI ScaI
SfaNI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI SspBI SspI
StuI StyI SwaI Tth111I Van91I
XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 210 GACGTC GACGT^C
Acc65I 338 GGTACC G^GTACC
AccI 345 GTCTAC GT^[AC][GT]AC
AcsI 887 AAATTC [GA]^AATT[CT]
ApoI 887 AAATTC [GA]^AATT[CT]
Asp718I 338 GGTACC G^GTACC
AspHI 238 GAGCAC G[AT]GC[AT]^C
BbeI 219 GGCGCC GGCGC^C
BbvCI 823 GCTGAGG CCTCAGC(-5/-2)
BfaI 1024 CTAG C^TAG
BsaBI 1405 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1438 GAGACC GGTCTC(1/5)
BsiHKAI 238 GAGCAC G[AT]GC[AT]^C
BsmAI 1438 GAGAC GTCTC(1/5)
BspHI 540 TCATGA T^CATGA
BsrBI 15 GAGCGG CCGCTC(-3/-3)
BssSI 501 CTCGTG CACGAG(-5/-1)
DsaI 1262 CCGTGG C^C[GA][CT]GG
FauI 17 GCGGG CCCGC(4/6)
HindIII 1063 AAGCTT A^AGCTT
KasI 219 GGCGCC G^GCGCC
KpnI 338 GGTACC GGTAC^C
MaeI 1024 CTAG C^TAG
MamI 1405 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 527 TTAA T^TAA
NarI 219 GGCGCC GG^CGCC
PpuMI 811 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 540 TCATGA T^CATGA
SbfI 1239 CCTGCAGG CCTGCA^GG
SexAI 1481 ACCTGGT A^CC[AT]GGT
SfoI 219 GGCGCC GGC^GCC
Sse8387I 1239 CCTGCAGG CCTGCA^GG
TfiI 496 GATTC G^A[AT]TC
Tru9I 527 TTAA T^TAA
XbaI 1023 TCTAGA T^CTAGA
XcmI 1397 CCAGGCCGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 210 GACGTC G[GA]^CG[CT]C
219 GGCGCC
AfaI 93 GTAC GT^AC
339 GTAC
AlwNI 148 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1477 CAGCACCTG
BlpI 51 GCTGAGC GC^T[ACGT]AGC
235 GCTGAGC
Bpu1102I 51 GCTGAGC GC^T[ACGT]AGC
235 GCTGAGC
BsaHI 210 GACGTC G[GA]^CG[CT]C
219 GGCGCC
BsgI 667 CTGCAC GTGCAG(16/14)
710 CTGCAC
BsmI 1165 GCATTC GAATGC(1/-1)
1233 GCATTC
BstAPI 24 GCAGGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1233 GCATTCCCTGC
BstXI 446 CCACCCCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1208 CCACCAAGATGG
BstYI 1270 AGATCC [GA]^GATC[CT]
1410 GGATCT
CelII 51 GCTGAGC GC^T[ACGT]AGC
235 GCTGAGC
CfoI 220 GCGC GCG^C
1490 GCGC
EcoNI 252 CCTGTGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
792 CCTGACAGAGG
HaeII 219 GGCGCC [GA]GCGC^[CT]
1489 AGCGCC
HhaI 220 GCGC GCG^C
1490 GCGC
Hin1I 210 GACGTC G[GA]^CG[CT]C
219 GGCGCC
HinP1I 220 GCGC G^CGC
1490 GCGC
MaeII 137 ACGT A^CGT
211 ACGT
MflI 1270 AGATCC [GA]^GATC[CT]
1410 GGATCT
RsaI 93 GTAC GT^AC
339 GTAC
XhoII 1270 AGATCC [GA]^GATC[CT]
1410 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AluI AlwI ApaI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
Sau3AI Sau96I ScrFI SfcI SmaI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I XmaCI XmaI