Restriction map for
pF1KE0467
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HinfI
HpaI HphI KasI KpnI KspI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp45I Tth111I
Van91I XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 25 enzymes) #
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AcsI 517 AAATTT [GA]^AATT[CT]
ApaI 53 GGGCCC GGGCC^C
ApoI 517 AAATTT [GA]^AATT[CT]
AspHI 538 GAGCAC G[AT]GC[AT]^C
BanI 397 GGTGCC G^G[CT][GA]CC
BanII 53 GGGCCC G[GA]GC[CT]^C
BcgI 558 CGACCATGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 10 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 578 CTCCAG CTGGAG(16/14)
BseRI 472 GAGGAG GAGGAG(10/8)
BsgI 408 CTGCAC GTGCAG(16/14)
BsiHKAI 538 GAGCAC G[AT]GC[AT]^C
DdeI 69 CTCAG C^T[ACGT]AG
EaeI 319 CGGCCA [CT]^GGCC[GA]
EcoT14I 401 CCATGG C^C[AT][AT]GG
MaeIII 304 GTTAC ^GT[ACGT]AC
NcoI 401 CCATGG C^CATGG
PpuMI 476 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 228 GACCAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmaI 48 CCCGGG CCC^GGG
SmlI 535 CTTGAG C^T[CT][GA]AG
StuI 552 AGGCCT AGG^CCT
StyI 401 CCATGG C^C[AT][AT]GG
XmaCI 48 CCCGGG C^CCGGG
XmaI 48 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Asp700I 282 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
496 GAACTTCTTC
AvaI 48 CCCGGG C^[CT]CG[GA]G
385 CTCGGG
BbvI 139 GCAGC GCAGC(8/12)
42 GCTGC
BmyI 53 GGGCCC G[AGT]GC[ACT]^C
538 GAGCAC
BsmFI 373 GGGAC GGGAC(10/14)
63 GTCCC
BsoBI 48 CCCGGG C^[CT]CG[GA]G
385 CTCGGG
Bsp1286I 53 GGGCCC G[AGT]GC[ACT]^C
538 GAGCAC
BstF5I 525 GGATG GGATG(2/0)
616 GGATG
BstNI 219 CCTGG CC^[AT]GG
609 CCAGG
Cac8I 172 GCAGGC GC[ACGT]^[ACGT]GC
406 GCCTGC
CfoI 8 GCGC GCG^C
45 GCGC
DraII 52 GGGGCCC [GA]G^G[ACGT]CC[CT]
476 AGGACCC
DsaI 401 CCATGG C^C[GA][CT]GG
413 CCGTGG
EcoO109I 52 GGGGCCC [GA]G^G[ACGT]CC[CT]
476 AGGACCC
EcoRII 219 CCTGG ^CC[AT]GG
609 CCAGG
FauI 29 CCCGC CCCGC(4/6)
370 GCGGG
FokI 525 GGATG GGATG(9/13)
616 GGATG
HgaI 462 GACGC GACGC(5/10)
233 GCGTC
HhaI 8 GCGC GCG^C
45 GCGC
HinP1I 8 GCGC G^CGC
45 GCGC
MaeII 307 ACGT A^CGT
347 ACGT
MspA1I 140 CAGCTG C[AC]G^C[GT]G
505 CAGCTG
MvaI 219 CCTGG CC^[AT]GG
609 CCAGG
PvuII 140 CAGCTG CAG^CTG
505 CAGCTG
SapI 529 GAAGAGC GCTCTTC(1/4)
597 GAAGAGC
TaqI 150 TCGA T^CGA
557 TCGA
TseI 42 GCTGC G^C[AT]GC
139 GCAGC
Tsp509I 518 AATT ^AATT
572 AATT
TspRI 90 CAGTG CA[GC]TG[ACGT][ACGT]^
362 CACTG
TthHB8I 150 TCGA T^CGA
557 TCGA
XmnI 282 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
496 GAACTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI AlwI AvaII BcnI
BsaJI BsiYI BslI BsrI BssKI
Cfr13I DpnII EarI Fnu4HI HaeIII
HapII HpaII ItaI Ksp632I MboI
MboII MnlI MspI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI