Restriction map for
pF1KE0122
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI ApaI
ApoI AscI AseI AsnI Asp700I
AspEI AviII AvrII BalI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaWI BseAI
BsgI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI Ksp632I MaeIII MamI MboII
MfeI MluI MluNI MroI MscI
MseI MslI MunI NdeI NotI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SapI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI SspBI SspI StuI
SwaI Tru9I Tsp45I Tsp509I TspRI
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 473 GGTACC G^GTACC
AcyI 97 GGCGCC G[GA]^CG[CT]C
Alw44I 484 GTGCAC G^TGCAC
Aor51HI 147 AGCGCT AGC^GCT
ApaLI 484 GTGCAC G^TGCAC
Asp718I 473 GGTACC G^GTACC
AspI 202 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 273 GGATCC G^GATCC
BbeI 97 GGCGCC GGCGC^C
BcgI 5 CGACTCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 343 CTCCAG CTGGAG(16/14)
BsaHI 97 GGCGCC G[GA]^CG[CT]C
BsiEI 369 CGGTCG CG[GA][CT]^CG
BsmI 595 GCATTC GAATGC(1/-1)
BsrBI 712 GAGCGG CCGCTC(-3/-3)
BsrI 568 ACTGG ACTGG(1/-1)
BstAPI 486 GCACGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 521 CCAACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 248 AGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 155 TGGCCG [CT]^GGCC[GA]
Eco47III 147 AGCGCT AGC^GCT
EcoO109I 248 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 658 CCATGG C^C[AT][AT]GG
HgaI 303 GCGTC GACGC(5/10)
Hin1I 97 GGCGCC G[GA]^CG[CT]C
HphI 543 GGTGA GGTGA(8/7)
KasI 97 GGCGCC G^GCGCC
KpnI 473 GGTACC GGTAC^C
KspI 167 CCGCGG CCGC^GG
MaeII 779 ACGT A^CGT
NarI 97 GGCGCC GG^CGCC
NcoI 658 CCATGG C^CATGG
NheI 453 GCTAGC G^CTAGC
NruI 372 TCGCGA TCG^CGA
NspI 491 ACATGC [GA]CATG^[CT]
PflFI 202 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 6 GACTC GAGTC(4/5)
PpuMI 248 AGGTCCT [GA]G^G[AT]CC[CT]
SacII 167 CCGCGG CCGC^GG
SbfI 294 CCTGCAGG CCTGCA^GG
SfoI 97 GGCGCC GGC^GCC
SmaI 45 CCCGGG CCC^GGG
Sse8387I 294 CCTGCAGG CCTGCA^GG
StyI 658 CCATGG C^C[AT][AT]GG
TfiI 465 GATTC G^A[AT]TC
Tth111I 202 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 45 CCCGGG C^CCGGG
XmaI 45 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 168 CGCG CG^CG
373 CGCG
AfaI 474 GTAC GT^AC
705 GTAC
AocI 252 CCTCAGG CC^T[ACGT]AGG
732 CCTGAGG
AspHI 422 GAGCAC G[AT]GC[AT]^C
484 GTGCAC
AvaI 45 CCCGGG C^[CT]CG[GA]G
419 CCCGAG
BbvI 12 GCTGC GCAGC(8/12)
138 GCTGC
BsiHKAI 422 GAGCAC G[AT]GC[AT]^C
484 GTGCAC
BsoBI 45 CCCGGG C^[CT]CG[GA]G
419 CCCGAG
BsrFI 100 GCCGGC [GA]^CCGG[CT]
160 GCCGGC
BstUI 168 CGCG CG^CG
373 CGCG
BstYI 273 GGATCC [GA]^GATC[CT]
290 AGATCC
Bsu36I 252 CCTCAGG CC^T[ACGT]AGG
732 CCTGAGG
Cfr10I 100 GCCGGC [GA]^CCGG[CT]
160 GCCGGC
DsaI 167 CCGCGG C^C[GA][CT]GG
658 CCATGG
Eco81I 252 CCTCAGG CC^T[ACGT]AGG
732 CCTGAGG
HinfI 6 GACTC G^A[ACGT]TC
465 GATTC
MflI 273 GGATCC [GA]^GATC[CT]
290 AGATCC
MvnI 168 CGCG CG^CG
373 CGCG
NaeI 100 GCCGGC GCC^GGC
160 GCCGGC
NgoMIV 100 GCCGGC G^CCGGC
160 GCCGGC
PflMI 198 CCACGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
783 CCAGGGCTTGG
PstI 139 CTGCAG CTGCA^G
295 CTGCAG
RsaI 474 GTAC GT^AC
705 GTAC
SfaNI 513 GATGC GCATC(5/9)
584 GCATC
TaqI 316 TCGA T^CGA
329 TCGA
TseI 12 GCTGC G^C[AT]GC
138 GCTGC
TthHB8I 316 TCGA T^CGA
329 TCGA
Van91I 198 CCACGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
783 CCAGGGCTTGG
XhoII 273 GGATCC [GA]^GATC[CT]
290 AGATCC
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanI BanII BcnI BfaI BmyI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI MaeI MboI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfcI XcmI