Restriction map for
pF1KA0034
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# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AcyI AgeI AhdI Aor51HI
ApaI AscI AspEI AviII AvrII
BbeI BcgI BglI BglII BlnI
BsaHI BsiEI BsiWI BsmBI BspLU11I
BsrBI BssHII Bst1107I BstAPI BstZ17I
CpoI EagI Eam1105I EclXI Eco47III
Eco52I FauI FseI FspI Hin1I
HindIII KasI KspI MfeI MluI
MunI NaeI NarI NcoI NgoMIV
NotI NruI PacI PinAI PmeI
PshAI PvuI RsrII SacII SbfI
SfiI SfoI SgrAI SmaI Sse8387I
SwaI XmaCI XmaI
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# ONE CUT ENZYMES ( 73 enzymes) #
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Acc65I 868 GGTACC G^GTACC
AccI 2429 GTCGAC GT^[AC][GT]AC
AccIII 3215 TCCGGA T^CCGGA
AflII 2287 CTTAAG C^TTAAG
Alw44I 2179 GTGCAC G^TGCAC
AocI 1437 CCTAAGG CC^T[ACGT]AGG
ApaLI 2179 GTGCAC G^TGCAC
AseI 2791 ATTAAT AT^TAAT
AsnI 2791 ATTAAT AT^TAAT
Asp718I 868 GGTACC G^GTACC
AspI 2344 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3491 CTCGAG C^[CT]CG[GA]G
BalI 2769 TGGCCA TGG^CCA
BbrPI 3113 CACGTG CAC^GTG
BbvCI 2539 GCTGAGG CCTCAGC(-5/-2)
BclI 3896 TGATCA T^GATCA
BfrI 2287 CTTAAG C^TTAAG
BlpI 4486 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4486 GCTCAGC GC^T[ACGT]AGC
BsaBI 2737 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3215 TCCGGA [AT]^CCGG[AT]
BseAI 3215 TCCGGA T^CCGGA
BsmI 1690 GCATTC GAATGC(1/-1)
BsoBI 3491 CTCGAG C^[CT]CG[GA]G
BspDI 2336 ATCGAT AT^CGAT
BspEI 3215 TCCGGA T^CCGGA
BsrFI 1072 GCCGGT [GA]^CCGG[CT]
BssSI 4298 CTCGTG CACGAG(-5/-1)
BstBI 185 TTCGAA TT^CGAA
BstEII 3604 GGTGACC G^GT[ACGT]ACC
BstPI 3604 GGTGACC G^GT[ACGT]ACC
Bsu36I 1437 CCTAAGG CC^T[ACGT]AGG
CelII 4486 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1072 GCCGGT [GA]^CCGG[CT]
ClaI 2336 ATCGAT AT^CGAT
DraI 2185 TTTAAA TTT^AAA
DraIII 1424 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 90 GACTATGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 4282 CCACGG C^C[GA][CT]GG
EaeI 2769 TGGCCA [CT]^GGCC[GA]
Eco81I 1437 CCTAAGG CC^T[ACGT]AGG
EcoNI 4596 CCTTTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3604 GGTGACC G^GT[ACGT]ACC
EcoRV 4177 GATATC GAT^ATC
EcoT22I 1768 ATGCAT ATGCA^T
FbaI 3896 TGATCA T^GATCA
HaeII 208 AGCGCC [GA]GCGC^[CT]
HgaI 1743 GACGC GACGC(5/10)
HpaI 2155 GTTAAC GTT^AAC
KpnI 868 GGTACC GGTAC^C
MamI 2737 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2769 TGGCCA TGG^CCA
MroI 3215 TCCGGA T^CCGGA
MscI 2769 TGGCCA TGG^CCA
NdeI 3284 CATATG CA^TATG
NsiI 1768 ATGCAT ATGCA^T
NspV 185 TTCGAA TT^CGAA
PaeR7I 3491 CTCGAG C^TCGAG
PflFI 2344 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3113 CACGTG CAC^GTG
PmlI 3113 CACGTG CAC^GTG
PshBI 2791 ATTAAT AT^TAAT
SacI 3932 GAGCTC GAGCT^C
SalI 2429 GTCGAC G^TCGAC
SfuI 185 TTCGAA TT^CGAA
SnaBI 1058 TACGTA TAC^GTA
SpeI 3436 ACTAGT A^CTAGT
SphI 1770 GCATGC GCATG^C
SspI 4030 AATATT AAT^ATT
StuI 1309 AGGCCT AGG^CCT
Tth111I 2344 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4021 TCTAGA T^CTAGA
XhoI 3491 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 1745 CGCG CG^CG
2707 CGCG
AclI 639 AACGTT AA^CGTT
3299 AACGTT
AflIII 3112 ACACGT A^C[GA][CT]GT
3114 ACGTGT
Asp700I 3415 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3789 GAAAGAATTC
BamHI 2146 GGATCC G^GATCC
2853 GGATCC
BanII 1829 GGGCTC G[GA]GC[CT]^C
3932 GAGCTC
BciVI 1525 GGATAC GTATCC(6/5)
1256 GTATCC
BsaAI 1058 TACGTA [CT]AC^GT[GA]
3113 CACGTG
BsaI 2901 GAGACC GGTCTC(1/5)
2128 GGTCTC
BseRI 1235 CTCCTC GAGGAG(10/8)
2611 GAGGAG
BspMI 871 ACCTGC ACCTGC(4/8)
3312 ACCTGC
BstUI 1745 CGCG CG^CG
2707 CGCG
BstXI 2857 CCAGAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3594 CCAACAAGTTGG
CfoI 209 GCGC GCG^C
1774 GCGC
HhaI 209 GCGC GCG^C
1774 GCGC
HinP1I 209 GCGC G^CGC
1774 GCGC
MspA1I 935 CAGCTG C[AC]G^C[GT]G
3716 CAGCTG
MvnI 1745 CGCG CG^CG
2707 CGCG
NheI 226 GCTAGC G^CTAGC
595 GCTAGC
Psp1406I 639 AACGTT AA^CGTT
3299 AACGTT
PvuII 935 CAGCTG CAG^CTG
3716 CAGCTG
SapI 1618 GAAGAGC GCTCTTC(1/4)
3889 GAAGAGC
SexAI 2837 ACCTGGT A^CC[AT]GGT
2915 ACCAGGT
Tsp45I 322 GTCAC ^GT[GC]AC
3605 GTGAC
XcmI 4144 CCAACTGATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4953 CCAGGCACCTTTTGG
XmnI 3415 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3789 GAAAGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspHI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I Van91I
XhoII