Restriction map for
pF1KB7179
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BanII BbeI BbsI BcgI BciVI
BfrI BlnI BlpI BpmI Bpu1102I
BpuAI BsaHI BsgI BsiWI BsmI
BsoBI Bsp1407I BspDI BspHI BspMI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII CfoI ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MfeI MluI MunI MvnI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI StuI
SwaI TaqI TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 793 TCCGGA T^CCGGA
AcsI 224 AAATTC [GA]^AATT[CT]
AflIII 1031 ACATGT A^C[GA][CT]GT
Alw44I 1253 GTGCAC G^TGCAC
AlwNI 383 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1231 CCTCAGG CC^T[ACGT]AGG
ApaLI 1253 GTGCAC G^TGCAC
ApoI 224 AAATTC [GA]^AATT[CT]
AspHI 1253 GTGCAC G[AT]GC[AT]^C
AspI 346 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 830 TGGCCA TGG^CCA
BanI 996 GGTGCC G^G[CT][GA]CC
BbrPI 276 CACGTG CAC^GTG
BbvCI 1074 CCTCAGC CCTCAGC(-5/-2)
BfaI 304 CTAG C^TAG
BglI 913 GCCACGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 576 AGATCT A^GATCT
BsaAI 276 CACGTG [CT]AC^GT[GA]
BsaI 804 GGTCTC GGTCTC(1/5)
BsaWI 793 TCCGGA [AT]^CCGG[AT]
BseAI 793 TCCGGA T^CCGGA
BsiEI 991 CGGCCG CG[GA][CT]^CG
BsiHKAI 1253 GTGCAC G[AT]GC[AT]^C
BsmBI 70 GAGACG CGTCTC(1/5)
BspEI 793 TCCGGA T^CCGGA
BspLU11I 1031 ACATGT A^CATGT
BssSI 331 CTCGTG CACGAG(-5/-1)
Bsu36I 1231 CCTCAGG CC^T[ACGT]AGG
DraII 706 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 615 CCATGG C^C[GA][CT]GG
EagI 991 CGGCCG C^GGCCG
EclXI 991 CGGCCG C^GGCCG
Eco52I 991 CGGCCG C^GGCCG
Eco81I 1231 CCTCAGG CC^T[ACGT]AGG
EcoO109I 706 AGGTCCT [GA]G^G[ACGT]CC[CT]
HgaI 743 GCGTC GACGC(5/10)
MaeI 304 CTAG C^TAG
MaeII 277 ACGT A^CGT
MluNI 830 TGGCCA TGG^CCA
MroI 793 TCCGGA T^CCGGA
MscI 830 TGGCCA TGG^CCA
NaeI 870 GCCGGC GCC^GGC
NcoI 615 CCATGG C^CATGG
NgoMIV 870 GCCGGC G^CCGGC
PflFI 346 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 276 CACGTG CAC^GTG
PmlI 276 CACGTG CAC^GTG
PpuMI 706 AGGTCCT [GA]G^G[AT]CC[CT]
SapI 133 GCTCTTC GCTCTTC(1/4)
SphI 620 GCATGC GCATG^C
SspI 1279 AATATT AAT^ATT
Tth111I 346 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BamHI 245 GGATCC G^GATCC
317 GGATCC
BcnI 672 CCCGG CC^[GC]GG
989 CCCGG
BmyI 886 GTGCCC G[AGT]GC[ACT]^C
1253 GTGCAC
BsaBI 265 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
960 GATGAATATC
BsmAI 70 GAGAC GTCTC(1/5)
805 GTCTC
Bsp1286I 886 GTGCCC G[AGT]GC[ACT]^C
1253 GTGCAC
BsrBI 1096 GAGCGG CCGCTC(-3/-3)
1430 GAGCGG
BstXI 821 CCATCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
969 CCACTTCTGTGG
EarI 134 CTCTTC CTCTTC(1/4)
699 GAAGAG
HincII 724 GTCAAC GT[CT]^[GA]AC
945 GTTGAC
HindII 724 GTCAAC GT[CT]^[GA]AC
945 GTTGAC
Ksp632I 134 CTCTTC CTCTTC(1/4)
699 GAAGAG
MamI 265 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
960 GATGAATATC
MspA1I 570 CAGCGG C[AC]G^C[GT]G
626 CCGCTG
NciI 672 CCCGG CC^[GC]GG
989 CCCGG
NspI 620 GCATGC [GA]CATG^[CT]
1031 ACATGT
PleI 307 GACTC GAGTC(4/5)
1135 GAGTC
PstI 65 CTGCAG CTGCA^G
178 CTGCAG
SfcI 65 CTGCAG C^T[GA][CT]AG
178 CTGCAG
TfiI 376 GAATC G^A[AT]TC
1370 GATTC
Tsp509I 225 AATT ^AATT
1324 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BclI BsaJI BseRI BsiYI
BslI BsmFI BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr10I
Cfr13I DdeI DpnII EaeI EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
Tru9I TseI Tsp45I TspRI XhoII