Restriction map for
pF1KE0493
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AseI AsnI Asp700I AspEI AspHI
AspI AviII AvrII BamHI BbrPI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiHKAI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KspI MaeII
MamI MfeI MluI MroI MseI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI TfiI Tru9I Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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Acc65I 581 GGTACC G^GTACC
AccI 690 GTCTAC GT^[AC][GT]AC
AcsI 1099 GAATTT [GA]^AATT[CT]
AlwI 594 GATCC GGATC(4/5)
AlwNI 598 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 672 CCTCAGG CC^T[ACGT]AGG
ApaI 394 GGGCCC GGGCC^C
ApoI 1099 GAATTT [GA]^AATT[CT]
AscI 107 GGCGCGCC GG^CGCGCC
Asp718I 581 GGTACC G^GTACC
BalI 53 TGGCCA TGG^CCA
BbeI 182 GGCGCC GGCGC^C
BcgI 930 GCATTTTATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 552 GCCCCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 970 GAGACC GGTCTC(1/5)
BsgI 190 GTGCAG GTGCAG(16/14)
BsmFI 568 GGGAC GGGAC(10/14)
Bsp1407I 644 TGTACA T^GTACA
BsrDI 498 CATTGC GCAATG(2/0)
BsrFI 425 GCCGGC [GA]^CCGG[CT]
BsrGI 644 TGTACA T^GTACA
BssHII 108 GCGCGC G^CGCGC
BssSI 868 CACGAG CACGAG(-5/-1)
BstAPI 493 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 503 CCACGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 593 AGATCC [GA]^GATC[CT]
Bsu36I 672 CCTCAGG CC^T[ACGT]AGG
Cfr10I 425 GCCGGC [GA]^CCGG[CT]
DpnII 594 GATC ^GATC
DsaI 572 CCATGG C^C[GA][CT]GG
EagI 5 CGGCCG C^GGCCG
EarI 244 CTCTTC CTCTTC(1/4)
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
Eco81I 672 CCTCAGG CC^T[ACGT]AGG
EcoNI 948 CCTGGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 130 GACGC GACGC(5/10)
KasI 182 GGCGCC G^GCGCC
KpnI 581 GGTACC GGTAC^C
Ksp632I 244 CTCTTC CTCTTC(1/4)
MboI 594 GATC ^GATC
MflI 593 AGATCC [GA]^GATC[CT]
MluNI 53 TGGCCA TGG^CCA
MscI 53 TGGCCA TGG^CCA
NaeI 425 GCCGGC GCC^GGC
NarI 182 GGCGCC GG^CGCC
NcoI 572 CCATGG C^CATGG
NdeII 594 GATC ^GATC
NgoMIV 425 GCCGGC G^CCGGC
PpuMI 599 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1164 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 243 GCTCTTC GCTCTTC(1/4)
Sau3AI 594 GATC ^GATC
ScaI 164 AGTACT AGT^ACT
SfcI 813 CTATAG C^T[GA][CT]AG
SfoI 182 GGCGCC GGC^GCC
SmaI 293 CCCGGG CCC^GGG
SspBI 644 TGTACA T^GTACA
StuI 228 AGGCCT AGG^CCT
Tsp45I 128 GTGAC ^GT[GC]AC
XcmI 555 CCACTGGCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 593 AGATCC [GA]^GATC[CT]
XmaCI 293 CCCGGG C^CCGGG
XmaI 293 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 109 CGCG CG^CG
125 CGCG
AcyI 130 GACGCC G[GA]^CG[CT]C
182 GGCGCC
AvaI 196 CCCGAG C^[CT]CG[GA]G
293 CCCGGG
BbsI 412 GTCTTC GAAGAC(2/6)
709 GTCTTC
BbvCI 231 CCTCAGC CCTCAGC(-5/-2)
204 GCTGAGG
BfaI 352 CTAG C^TAG
685 CTAG
BpmI 1075 CTCCAG CTGGAG(16/14)
637 CTGGAG
BpuAI 412 GTCTTC GAAGAC(2/6)
709 GTCTTC
BsaHI 130 GACGCC G[GA]^CG[CT]C
182 GGCGCC
BsiEI 5 CGGCCG CG[GA][CT]^CG
385 CGGTCG
BsmAI 970 GAGAC GTCTC(1/5)
1125 GTCTC
BsoBI 196 CCCGAG C^[CT]CG[GA]G
293 CCCGGG
BstUI 109 CGCG CG^CG
125 CGCG
EcoT14I 545 CCAAGG C^C[AT][AT]GG
572 CCATGG
FauI 32 GCGGG CCCGC(4/6)
173 GCGGG
HaeII 137 AGCGCC [GA]GCGC^[CT]
182 GGCGCC
Hin1I 130 GACGCC G[GA]^CG[CT]C
182 GGCGCC
HphI 967 GGTGA GGTGA(8/7)
542 TCACC
MaeI 352 CTAG C^TAG
685 CTAG
MvnI 109 CGCG CG^CG
125 CGCG
SmlI 464 CTCAAG C^T[CT][GA]AG
914 CTCAAG
StyI 545 CCAAGG C^C[AT][AT]GG
572 CCATGG
TaqI 474 TCGA T^CGA
794 TCGA
Tsp509I 518 AATT ^AATT
1100 AATT
TthHB8I 474 TCGA T^CGA
794 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BanII BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DraII
EaeI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeIII MboII MnlI
MslI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV PleI PvuII
RsaI Sau96I ScrFI SfaNI TseI
TspRI