Restriction map for
pF1KB7323
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# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AscI AseI AsnI Asp718I
AviII AvrII BfrI BglII BlnI
BsaBI BsiWI BspLU11I Bst1107I BstBI
BstZ17I CpoI DraI EcoRI EcoT22I
FseI FspI HindIII HpaI KpnI
MamI MfeI MluI MunI NdeI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI SfiI
SfuI SnaBI Sse8387I SwaI XbaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 5417 TCCGGA T^CCGGA
AflIII 2804 ACGTGT A^C[GA][CT]GT
AhdI 67 GACGAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1739 AGCGCT AGC^GCT
AspEI 67 GACGAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3337 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1172 GGATCC G^GATCC
BcgI 939 CGAATGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5179 GTATCC GTATCC(6/5)
BclI 1832 TGATCA T^GATCA
BsaI 4450 GAGACC GGTCTC(1/5)
BseAI 5417 TCCGGA T^CCGGA
BsiEI 812 CGGCCG CG[GA][CT]^CG
BsmI 940 GAATGC GAATGC(1/-1)
Bsp1407I 5765 TGTACA T^GTACA
BspDI 1876 ATCGAT AT^CGAT
BspEI 5417 TCCGGA T^CCGGA
BspHI 2954 TCATGA T^CATGA
BsrGI 5765 TGTACA T^GTACA
BssHII 2264 GCGCGC G^CGCGC
BssSI 4822 CACGAG CACGAG(-5/-1)
ClaI 1876 ATCGAT AT^CGAT
DrdI 2041 GACCCCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 812 CGGCCG C^GGCCG
Eam1105I 67 GACGAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 812 CGGCCG C^GGCCG
Eco47III 1739 AGCGCT AGC^GCT
Eco52I 812 CGGCCG C^GGCCG
EcoRV 5857 GATATC GAT^ATC
FbaI 1832 TGATCA T^GATCA
MroI 5417 TCCGGA T^CCGGA
NaeI 2820 GCCGGC GCC^GGC
NgoMIV 2820 GCCGGC G^CCGGC
NheI 675 GCTAGC G^CTAGC
PaeR7I 867 CTCGAG C^TCGAG
PflFI 3337 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2954 TCATGA T^CATGA
ScaI 2873 AGTACT AGT^ACT
SgrAI 5209 CACCGGCG C[GA]^CCGG[CT]G
SpeI 4524 ACTAGT A^CTAGT
SphI 4319 GCATGC GCATG^C
SspBI 5765 TGTACA T^GTACA
SspI 4112 AATATT AAT^ATT
Tth111I 3337 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 867 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 35 enzymes) #
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AcsI 2452 AAATTC [GA]^AATT[CT]
5098 AAATTC
Alw44I 1822 GTGCAC G^TGCAC
1863 GTGCAC
AocI 501 CCTCAGG CC^T[ACGT]AGG
4591 CCTGAGG
ApaLI 1822 GTGCAC G^TGCAC
1863 GTGCAC
ApoI 2452 AAATTC [GA]^AATT[CT]
5098 AAATTC
Asp700I 2418 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5355 GAACAAGTTC
BalI 3326 TGGCCA TGG^CCA
3482 TGGCCA
BbeI 2313 GGCGCC GGCGC^C
5213 GGCGCC
BbrPI 2458 CACGTG CAC^GTG
4495 CACGTG
BbvCI 1995 GCTGAGG CCTCAGC(-5/-2)
5014 GCTGAGG
BfaI 676 CTAG C^TAG
4525 CTAG
BsaWI 3800 ACCGGA [AT]^CCGG[AT]
5417 TCCGGA
BsmBI 652 GAGACG CGTCTC(1/5)
4870 GAGACG
BstEII 657 GGTCACC G^GT[ACGT]ACC
2331 GGTCACC
BstPI 657 GGTCACC G^GT[ACGT]ACC
2331 GGTCACC
Bsu36I 501 CCTCAGG CC^T[ACGT]AGG
4591 CCTGAGG
Eco81I 501 CCTCAGG CC^T[ACGT]AGG
4591 CCTGAGG
EcoO65I 657 GGTCACC G^GT[ACGT]ACC
2331 GGTCACC
KasI 2313 GGCGCC G^GCGCC
5213 GGCGCC
KspI 34 CCGCGG CCGC^GG
614 CCGCGG
MaeI 676 CTAG C^TAG
4525 CTAG
MluNI 3326 TGGCCA TGG^CCA
3482 TGGCCA
MscI 3326 TGGCCA TGG^CCA
3482 TGGCCA
NarI 2313 GGCGCC GG^CGCC
5213 GGCGCC
NcoI 2068 CCATGG C^CATGG
5465 CCATGG
PmaCI 2458 CACGTG CAC^GTG
4495 CACGTG
PmlI 2458 CACGTG CAC^GTG
4495 CACGTG
PstI 947 CTGCAG CTGCA^G
5674 CTGCAG
SacII 34 CCGCGG CCGC^GG
614 CCGCGG
SapI 963 GAAGAGC GCTCTTC(1/4)
4374 GAAGAGC
SexAI 2255 ACCTGGT A^CC[AT]GGT
3509 ACCTGGT
SfoI 2313 GGCGCC GGC^GCC
5213 GGCGCC
Tsp509I 2453 AATT ^AATT
5099 AATT
XcmI 1351 CCAAACGGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3554 CCAAGCAGGAGCTGG
XmnI 2418 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5355 GAACAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XhoII XmaCI XmaI