##################################
# NO CUT ENZYMES (  203 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FokI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HinfI       HpaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tru9I       Tsp45I      Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

BanI          250  GGTGCC               G^G[CT][GA]CC
BmyI          251  GTGCCC               G[AGT]GC[ACT]^C
BsmFI          13  GTCCC                GGGAC(10/14)
Bsp1286I      251  GTGCCC               G[AGT]GC[ACT]^C
BsrBI          90  CCGCTC               CCGCTC(-3/-3)
BsrDI         156  GCAATG               GCAATG(2/0)
BsrFI         247  GCCGGT               [GA]^CCGG[CT]
BsrI          149  ACTGG                ACTGG(1/-1)
Cfr10I        247  GCCGGT               [GA]^CCGG[CT]
DdeI            9  CTCAG                C^T[ACGT]AG
DpnII          84  GATC                 ^GATC
DraII          65  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO109I       65  AGGACCC              [GA]G^G[ACGT]CC[CT]
HapII         248  CCGG                 C^CGG
HincII          3  GTCAAC               GT[CT]^[GA]AC
HindII          3  GTCAAC               GT[CT]^[GA]AC
HpaII         248  CCGG                 C^CGG
HphI           42  GGTGA                GGTGA(8/7)
MboI           84  GATC                 ^GATC
MboII          45  GAAGA                GAAGA(8/7)
MspI          248  CCGG                 C^CGG
NdeII          84  GATC                 ^GATC
PpuMI          65  AGGACCC              [GA]G^G[AT]CC[CT]
PstI          226  CTGCAG               CTGCA^G
PvuII          79  CAGCTG               CAG^CTG
Sau3AI         84  GATC                 ^GATC
SfcI          226  CTGCAG               C^T[GA][CT]AG
SmlI           17  CTCAAG               C^T[CT][GA]AG
TspRI         119  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AluI           80  AGCT                 AG^CT
              224  AGCT                
BbvI          103  GCTGC                GCAGC(8/12)
              225  GCTGC               
BsgI          121  GTGCAG               GTGCAG(16/14)
              172  GTGCAG              
BsiYI         138  CCAGCACCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              162  CCAGCCACAGG         
BslI          138  CCAGCACCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              162  CCAGCCACAGG         
BssKI          29  CCTGG                ^CC[ACGT]GG
              144  CCAGG               
BstNI          29  CCTGG                CC^[AT]GG
              144  CCAGG               
EcoRII         29  CCTGG                ^CC[AT]GG
              144  CCAGG               
FauI           69  CCCGC                CCCGC(4/6)
               89  CCCGC               
HaeIII         22  GGCC                 GG^CC
              135  GGCC                
MspA1I         70  CCGCTG               C[AC]G^C[GT]G
               79  CAGCTG              
MvaI           29  CCTGG                CC^[AT]GG
              144  CCAGG               
NlaIII        132  CATG                 CATG^
              192  CATG                
ScrFI          29  CCTGG                CC^[ACGT]GG
              144  CCAGG               
TseI          103  GCTGC                G^C[AT]GC
              225  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AciI        AvaII       BseRI       Cac8I       Cfr13I      
Fnu4HI      ItaI        MnlI        MwoI        NlaIV       
Sau96I