Restriction map for
pF1KE0137
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AvaII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I Cac8I CelII Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI HaeII
HgaI Hin1I HincII HindII HinfI
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MroI MslI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 382 CGCG CG^CG
AciI 124 GCGG CCGC(-3/-1)
AflIII 381 ACGCGT A^C[GA][CT]GT
AlwI 96 GATCC GGATC(4/5)
ApaI 173 GGGCCC GGGCC^C
Asp700I 321 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 488 TGCGCA TGC^GCA
BalI 386 TGGCCA TGG^CCA
BanI 136 GGCACC G^G[CT][GA]CC
BbvI 423 GCTGC GCAGC(8/12)
BcnI 99 CCGGG CC^[GC]GG
BseRI 418 GAGGAG GAGGAG(10/8)
BsmFI 238 GGGAC GGGAC(10/14)
BspHI 494 TCATGA T^CATGA
BsrDI 166 GCAATG GCAATG(2/0)
BstF5I 126 GGATG GGATG(2/0)
BstNI 177 CCTGG CC^[AT]GG
BstUI 382 CGCG CG^CG
BstXI 389 CCAAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 95 AGATCC [GA]^GATC[CT]
CfoI 489 GCGC GCG^C
DraI 13 TTTAAA TTT^AAA
EaeI 386 TGGCCA [CT]^GGCC[GA]
EarI 79 GAAGAG CTCTTC(1/4)
EcoRII 177 CCTGG ^CC[AT]GG
EcoT14I 389 CCAAGG C^C[AT][AT]GG
Fnu4HI 423 GCTGC GC^[ACGT]GC
FokI 126 GGATG GGATG(9/13)
FspI 488 TGCGCA TGC^GCA
HapII 99 CCGG C^CGG
HhaI 489 GCGC GCG^C
HinP1I 489 GCGC G^CGC
HpaII 99 CCGG C^CGG
ItaI 423 GCTGC GC^[ACGT]GC
Ksp632I 79 GAAGAG CTCTTC(1/4)
MaeIII 260 GTGAC ^GT[ACGT]AC
MflI 95 AGATCC [GA]^GATC[CT]
MluI 381 ACGCGT A^CGCGT
MluNI 386 TGGCCA TGG^CCA
MscI 386 TGGCCA TGG^CCA
MspI 99 CCGG C^CGG
MvaI 177 CCTGG CC^[AT]GG
MvnI 382 CGCG CG^CG
NciI 99 CCGGG CC^[GC]GG
PstI 424 CTGCAG CTGCA^G
RcaI 494 TCATGA T^CATGA
SapI 79 GAAGAGC GCTCTTC(1/4)
SfcI 424 CTGCAG C^T[GA][CT]AG
StyI 389 CCAAGG C^C[AT][AT]GG
TseI 423 GCTGC G^C[AT]GC
Tsp45I 260 GTGAC ^GT[GC]AC
TspRI 471 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 95 AGATCC [GA]^GATC[CT]
XmnI 321 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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BanII 57 GAGCCC G[GA]GC[CT]^C
173 GGGCCC
BmyI 57 GAGCCC G[AGT]GC[ACT]^C
173 GGGCCC
Bsp1286I 57 GAGCCC G[AGT]GC[ACT]^C
173 GGGCCC
BsrI 133 ACTGG ACTGG(1/-1)
352 ACTGG
BssKI 99 CCGGG ^CC[ACGT]GG
177 CCTGG
Cfr13I 173 GGGCC G^G[ACGT]CC
174 GGCCC
DraII 172 AGGGCCC [GA]G^G[ACGT]CC[CT]
173 GGGCCCT
EcoO109I 172 AGGGCCC [GA]G^G[ACGT]CC[CT]
173 GGGCCCT
HindIII 104 AAGCTT A^AGCTT
323 AAGCTT
HphI 259 GGTGA GGTGA(8/7)
400 GGTGA
MwoI 22 GCAAACATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
166 GCAATGAGGGC
NlaIII 27 CATG CATG^
495 CATG
NlaIV 136 GGCACC GG[ACGT]^[ACGT]CC
173 GGGCCC
Sau96I 173 GGGCC G^G[ACGT]CC
174 GGCCC
ScrFI 99 CCGGG CC^[ACGT]GG
177 CCTGG
TaqI 119 TCGA T^CGA
450 TCGA
Tsp509I 204 AATT ^AATT
225 AATT
TthHB8I 119 TCGA T^CGA
450 TCGA
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# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
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AluI BsaJI DdeI DpnII HaeIII
MboI MboII MnlI MseI NdeII
Sau3AI SfaNI Tru9I