Restriction map for
pF1KB6312
##################################
# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MamI MfeI
MluI MroI MslI MspI MunI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccII 196 CGCG CG^CG
AfaI 608 GTAC GT^AC
AlwNI 241 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 830 GGACC G^G[AT]CC
AvrII 7 CCTAGG C^CTAGG
BamHI 762 GGATCC G^GATCC
BbsI 586 GTCTTC GAAGAC(2/6)
BfaI 8 CTAG C^TAG
BglII 677 AGATCT A^GATCT
BlnI 7 CCTAGG C^CTAGG
BpuAI 586 GTCTTC GAAGAC(2/6)
BseRI 136 CTCCTC GAGGAG(10/8)
BsiEI 84 CGACCG CG[GA][CT]^CG
BsmAI 803 GTCTC GTCTC(1/5)
BsmBI 802 CGTCTC CGTCTC(1/5)
BsrBI 625 GAGCGG CCGCTC(-3/-3)
BstF5I 440 CATCC GGATG(2/0)
BstUI 196 CGCG CG^CG
CfoI 195 GCGC GCG^C
DraII 242 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 577 GACTCAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 242 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 274 GATATC GAT^ATC
FokI 440 CATCC GGATG(9/13)
HhaI 195 GCGC GCG^C
HinP1I 195 GCGC G^CGC
HphI 278 TCACC GGTGA(8/7)
MaeI 8 CTAG C^TAG
MaeII 399 ACGT A^CGT
MseI 785 TTAA T^TAA
MvnI 196 CGCG CG^CG
NcoI 539 CCATGG C^CATGG
NlaIII 540 CATG CATG^
PstI 816 CTGCAG CTGCA^G
RsaI 608 GTAC GT^AC
SfcI 816 CTGCAG C^T[GA][CT]AG
Tru9I 785 TTAA T^TAA
Tsp45I 731 GTGAC ^GT[GC]AC
Tsp509I 114 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Asp700I 75 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
304 GAAGCTATTC
BalI 29 TGGCCA TGG^CCA
725 TGGCCA
BpmI 554 CTCCAG CTGGAG(16/14)
611 CTCCAG
DdeI 455 CTCAG C^T[ACGT]AG
642 CTCAG
DsaI 518 CCGTGG C^C[GA][CT]GG
539 CCATGG
EaeI 29 TGGCCA [CT]^GGCC[GA]
725 TGGCCA
MaeIII 508 GTTAC ^GT[ACGT]AC
731 GTGAC
MluNI 29 TGGCCA TGG^CCA
725 TGGCCA
MscI 29 TGGCCA TGG^CCA
725 TGGCCA
MspA1I 263 CAGCTG C[AC]G^C[GT]G
757 CAGCTG
MwoI 256 GCGGCAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
704 GCGGAGAGAGC
PvuII 263 CAGCTG CAG^CTG
757 CAGCTG
SmlI 139 CTCAAG C^T[CT][GA]AG
579 CTCAAG
StuI 558 AGGCCT AGG^CCT
770 AGGCCT
TaqI 83 TCGA T^CGA
167 TCGA
TfiI 104 GAATC G^A[AT]TC
405 GAATC
TthHB8I 83 TCGA T^CGA
167 TCGA
XmnI 75 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
304 GAAGCTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BsiYI BslI BsrI BssKI BstNI
BstYI Cac8I Cfr13I DpnII EarI
EcoNI EcoRII EcoT14I Fnu4HI HaeIII
HinfI ItaI Ksp632I MboI MboII
MflI MnlI MvaI NdeII NlaIV
PleI Sau3AI Sau96I ScrFI StyI
TseI TspRI XhoII