Restriction map for
pF1KB8655
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BanI BbeI BciVI
BclI BfrI BglI BglII BlnI
BsaBI BsaHI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
BstBI BstEII BstPI Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 1069 CGCG CG^CG
Asp700I 199 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 835 GTGCTC G[AT]GC[AT]^C
AvaI 1159 CCCGAG C^[CT]CG[GA]G
BalI 1115 TGGCCA TGG^CCA
BanII 1155 GAGCCC G[GA]GC[CT]^C
BbrPI 130 CACGTG CAC^GTG
BbvCI 1651 GCTGAGG CCTCAGC(-5/-2)
BcgI 1304 GCACCAACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1382 CTAG C^TAG
BlpI 1404 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1404 GCTCAGC GC^T[ACGT]AGC
BsaI 1534 GAGACC GGTCTC(1/5)
BsaWI 761 ACCGGA [AT]^CCGG[AT]
BsiHKAI 835 GTGCTC G[AT]GC[AT]^C
BsoBI 1159 CCCGAG C^[CT]CG[GA]G
BsrDI 1287 CATTGC GCAATG(2/0)
Bst1107I 325 GTATAC GTA^TAC
BstAPI 498 GCAAACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1069 CGCG CG^CG
BstXI 1118 CCAGCGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 325 GTATAC GTA^TAC
CelII 1404 GCTCAGC GC^T[ACGT]AGC
EaeI 1115 TGGCCA [CT]^GGCC[GA]
EcoNI 963 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 1675 GACGC GACGC(5/10)
HinfI 1036 GAGTC G^A[ACGT]TC
MaeI 1382 CTAG C^TAG
MluNI 1115 TGGCCA TGG^CCA
MscI 1115 TGGCCA TGG^CCA
MseI 356 TTAA T^TAA
MvnI 1069 CGCG CG^CG
NaeI 1386 GCCGGC GCC^GGC
NgoMIV 1386 GCCGGC G^CCGGC
PleI 1036 GAGTC GAGTC(4/5)
PmaCI 130 CACGTG CAC^GTG
PmlI 130 CACGTG CAC^GTG
PshAI 949 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 733 CTGCAG CTGCA^G
SapI 421 GAAGAGC GCTCTTC(1/4)
SfcI 733 CTGCAG C^T[GA][CT]AG
StuI 110 AGGCCT AGG^CCT
TaqI 1438 TCGA T^CGA
Tru9I 356 TTAA T^TAA
TthHB8I 1438 TCGA T^CGA
XmnI 199 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 241 GTAGAC GT^[AC][GT]AC
325 GTATAC
AcsI 1014 GAATTT [GA]^AATT[CT]
1048 AAATTT
AfaI 1236 GTAC GT^AC
1338 GTAC
AlwNI 152 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1195 CAGGCTCTG
ApoI 1014 GAATTT [GA]^AATT[CT]
1048 AAATTT
AspI 585 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1123 GACGTGGTC
BcnI 536 CCCGG CC^[GC]GG
1604 CCCGG
BsaAI 130 CACGTG [CT]AC^GT[GA]
712 TACGTG
BsmAI 545 GAGAC GTCTC(1/5)
1534 GAGAC
BsmI 426 GCATTC GAATGC(1/-1)
1085 GCATTC
BspMI 1415 ACCTGC ACCTGC(4/8)
969 GCAGGT
BsrFI 1386 GCCGGC [GA]^CCGG[CT]
1448 ACCGGC
BstYI 101 AGATCC [GA]^GATC[CT]
806 GGATCT
Cfr10I 1386 GCCGGC [GA]^CCGG[CT]
1448 ACCGGC
HaeII 737 AGCGCC [GA]GCGC^[CT]
1451 GGCGCT
MflI 101 AGATCC [GA]^GATC[CT]
806 GGATCT
NciI 536 CCCGG CC^[GC]GG
1604 CCCGG
NspI 644 ACATGC [GA]CATG^[CT]
1064 ACATGC
PflFI 585 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1123 GACGTGGTC
PpuMI 53 GGGTCCT [GA]G^G[AT]CC[CT]
632 AGGACCT
PvuII 1264 CAGCTG CAG^CTG
1293 CAGCTG
RsaI 1236 GTAC GT^AC
1338 GTAC
SmlI 187 CTTGAG C^T[CT][GA]AG
1420 CTCAAG
Tth111I 585 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1123 GACGTGGTC
XhoII 101 AGATCC [GA]^GATC[CT]
806 GGATCT
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AluI AlwI AvaII BbsI
BbvI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV PflMI Sau3AI Sau96I ScrFI
SfaNI StyI TseI Tsp45I Tsp509I
TspRI Van91I XcmI