Restriction map for
pF1KE0305
##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccII AclI AcsI AflII
AgeI Alw44I AlwI ApaLI ApoI
AscI AseI AsnI AspI AviII
BamHI BbeI BbsI BbvCI BciVI
BclI BfrI BglII BpuAI BsaBI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstBI BstUI BstYI BstZ17I
ClaI CpoI DpnII DraI DraIII
DrdI EagI EclXI Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KasI KspI MamI
MboI MfeI MflI MluI MseI
MunI MvnI NarI NdeI NdeII
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI Sau3AI SfoI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
StuI SwaI Tru9I Tsp509I Tth111I
XbaI XhoII
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 998 GGTACC G^GTACC
AccI 1550 GTAGAC GT^[AC][GT]AC
AccIII 395 TCCGGA T^CCGGA
AflIII 977 ACACGT A^C[GA][CT]GT
AhdI 696 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 232 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 237 CCTGAGG CC^T[ACGT]AGG
Asp700I 333 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 998 GGTACC G^GTACC
AspEI 696 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1758 CCTAGG C^CTAGG
BcgI 907 CGAAACCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1758 CCTAGG C^CTAGG
BlpI 129 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 129 GCTGAGC GC^T[ACGT]AGC
BsaI 1392 GGTCTC GGTCTC(1/5)
BsaWI 395 TCCGGA [AT]^CCGG[AT]
BseAI 395 TCCGGA T^CCGGA
BsgI 1197 CTGCAC GTGCAG(16/14)
BspEI 395 TCCGGA T^CCGGA
BspMI 1684 GCAGGT ACCTGC(4/8)
BsrBI 451 CCGCTC CCGCTC(-3/-3)
BsrFI 563 GCCGGC [GA]^CCGG[CT]
BssSI 402 CTCGTG CACGAG(-5/-1)
BstAPI 1734 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 237 CCTGAGG CC^T[ACGT]AGG
CelII 129 GCTGAGC GC^T[ACGT]AGC
Cfr10I 563 GCCGGC [GA]^CCGG[CT]
Eam1105I 696 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 237 CCTGAGG CC^T[ACGT]AGG
EcoNI 714 CCTGTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 1075 GTCAAC GT[CT]^[GA]AC
HindII 1075 GTCAAC GT[CT]^[GA]AC
KpnI 998 GGTACC GGTAC^C
MroI 395 TCCGGA T^CCGGA
NaeI 563 GCCGGC GCC^GGC
NgoMIV 563 GCCGGC G^CCGGC
NspI 557 GCATGT [GA]CATG^[CT]
PaeR7I 1011 CTCGAG C^TCGAG
PstI 1682 CTGCAG CTGCA^G
SacI 391 GAGCTC GAGCT^C
SapI 189 GAAGAGC GCTCTTC(1/4)
SbfI 1681 CCTGCAGG CCTGCA^GG
ScaI 222 AGTACT AGT^ACT
SexAI 887 ACCTGGT A^CC[AT]GGT
SfcI 1682 CTGCAG C^T[GA][CT]AG
SfiI 1297 GGCCCGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1681 CCTGCAGG CCTGCA^GG
TfiI 869 GATTC G^A[AT]TC
XhoI 1011 CTCGAG C^TCGAG
XmnI 333 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 1570 GACGCC G[GA]^CG[CT]C
1624 GGCGTC
Aor51HI 133 AGCGCT AGC^GCT
416 AGCGCT
BbrPI 945 CACGTG CAC^GTG
978 CACGTG
BfaI 320 CTAG C^TAG
1759 CTAG
BsaAI 945 CACGTG [CT]AC^GT[GA]
978 CACGTG
BsaHI 1570 GACGCC G[GA]^CG[CT]C
1624 GGCGTC
BstEII 594 GGTGACC G^GT[ACGT]ACC
891 GGTGACC
BstPI 594 GGTGACC G^GT[ACGT]ACC
891 GGTGACC
BstXI 5 CCACCTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1621 CCAGGCGTCTGG
EarI 189 GAAGAG CTCTTC(1/4)
1833 GAAGAG
Eco47III 133 AGCGCT AGC^GCT
416 AGCGCT
EcoO65I 594 GGTGACC G^GT[ACGT]ACC
891 GGTGACC
HaeII 133 AGCGCT [GA]GCGC^[CT]
416 AGCGCT
Hin1I 1570 GACGCC G[GA]^CG[CT]C
1624 GGCGTC
Ksp632I 189 GAAGAG CTCTTC(1/4)
1833 GAAGAG
MaeI 320 CTAG C^TAG
1759 CTAG
MslI 6 CACCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1764 CACCGTGGTG
PmaCI 945 CACGTG CAC^GTG
978 CACGTG
PmlI 945 CACGTG CAC^GTG
978 CACGTG
PshAI 316 GACCCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1009 GACTCGAGTC
SmaI 52 CCCGGG CCC^GGG
1299 CCCGGG
XcmI 623 CCAGTTCCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
944 CCACGTGCGTCCTGG
XmaCI 52 CCCGGG C^CCGGG
1299 CCCGGG
XmaI 52 CCCGGG C^CCGGG
1299 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AfaI AluI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboII MluNI
MnlI MscI MspA1I MspI MvaI
MwoI NciI NcoI NlaIII NlaIV
PflMI PleI PpuMI PvuII RsaI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I