Restriction map for
pF1KE0444
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaII
AviII AvrII BalI BamHI BanII
BbeI BbrPI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmFI
BsmI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssKI
BssSI Bst1107I BstAPI BstNI BstUI
BstXI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco52I EcoNI EcoRI EcoRII
EcoRV FseI FspI HapII HgaI
Hin1I HincII HindII HindIII HinfI
HpaI HpaII KasI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MluNI MroI MscI MseI MspA1I
MspI MunI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScrFI SexAI SfcI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AlwNI 731 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 540 CCTTAGG CC^T[ACGT]AGG
Aor51HI 273 AGCGCT AGC^GCT
Asp700I 462 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 269 CCCGAG C^[CT]CG[GA]G
BanI 577 GGCACC G^G[CT][GA]CC
BbsI 13 GAAGAC GAAGAC(2/6)
BbvCI 340 GCTGAGG CCTCAGC(-5/-2)
BclI 99 TGATCA T^GATCA
BpuAI 13 GAAGAC GAAGAC(2/6)
BsaJI 522 CCAAGG C^C[ACGT][ACGT]GG
BsiYI 270 CCGAGCGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 270 CCGAGCGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 310 CGTCTC CGTCTC(1/5)
BsoBI 269 CCCGAG C^[CT]CG[GA]G
BstBI 668 TTCGAA TT^CGAA
BstEII 426 GGTTACC G^GT[ACGT]ACC
BstPI 426 GGTTACC G^GT[ACGT]ACC
Bsu36I 540 CCTTAGG CC^T[ACGT]AGG
DraII 808 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eco47III 273 AGCGCT AGC^GCT
Eco81I 540 CCTTAGG CC^T[ACGT]AGG
EcoO109I 808 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 426 GGTTACC G^GT[ACGT]ACC
EcoT14I 522 CCAAGG C^C[AT][AT]GG
EcoT22I 65 ATGCAT ATGCA^T
FauI 718 GCGGG CCCGC(4/6)
FbaI 99 TGATCA T^GATCA
HaeII 273 AGCGCT [GA]GCGC^[CT]
MaeII 309 ACGT A^CGT
MslI 106 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIV 577 GGCACC GG[ACGT]^[ACGT]CC
NsiI 65 ATGCAT ATGCA^T
NspI 17 ACATGC [GA]CATG^[CT]
NspV 668 TTCGAA TT^CGAA
ScaI 444 AGTACT AGT^ACT
SfaNI 47 GCATC GCATC(5/9)
SfuI 668 TTCGAA TT^CGAA
StuI 525 AGGCCT AGG^CCT
StyI 522 CCAAGG C^C[AT][AT]GG
XcmI 641 CCAAGTTTCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 462 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 186 CCGC CCGC(-3/-1)
718 GCGG
BsmAI 311 GTCTC GTCTC(1/5)
766 GTCTC
BstF5I 590 CATCC GGATG(2/0)
396 GGATG
CfoI 274 GCGC GCG^C
498 GCGC
Cfr13I 345 GGGCC G^G[ACGT]CC
809 GGGCC
DpnII 100 GATC ^GATC
249 GATC
FokI 590 CATCC GGATG(9/13)
396 GGATG
HhaI 274 GCGC GCG^C
498 GCGC
HinP1I 274 GCGC G^CGC
498 GCGC
HphI 115 GGTGA GGTGA(8/7)
130 TCACC
MboI 100 GATC ^GATC
249 GATC
NdeII 100 GATC ^GATC
249 GATC
Sau3AI 100 GATC ^GATC
249 GATC
Sau96I 345 GGGCC G^G[ACGT]CC
809 GGGCC
TaqI 36 TCGA T^CGA
669 TCGA
Tsp45I 73 GTCAC ^GT[GC]AC
473 GTGAC
TthHB8I 36 TCGA T^CGA
669 TCGA
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
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AfaI AluI BbvI BfaI Cac8I
DdeI Fnu4HI HaeIII ItaI MaeI
MaeIII MboII MnlI NlaIII RsaI
TseI Tsp509I TspRI