##################################
# NO CUT ENZYMES (  217 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        AluI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      Cfr13I      
ClaI        CpoI        DdeI        DpnII       DraI        
DraII       DraIII      DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        Hin1I       HincII      HindII      HindIII     
HinfI       HpaI        HpaII       KasI        KpnI        
Ksp632I     MaeI        MaeII       MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspI        MunI        
MvaI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NlaIII      
NlaIV       NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SalI        SapI        Sau3AI      Sau96I      SbfI        
ScaI        ScrFI       SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AccII          12  CGCG                 CG^CG
AfaI          249  GTAC                 GT^AC
AspHI         168  GTGCTC               G[AT]GC[AT]^C
BsaI            6  GGTCTC               GGTCTC(1/5)
BsiHKAI       168  GTGCTC               G[AT]GC[AT]^C
BsiYI         135  CCACATCAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          135  CCACATCAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI           7  GTCTC                GTCTC(1/5)
BsrI          158  CCAGT                ACTGG(1/-1)
BstF5I         30  CATCC                GGATG(2/0)
BstUI          12  CGCG                 CG^CG
Cac8I         115  GCCCGC               GC[ACGT]^[ACGT]GC
CfoI           53  GCGC                 GCG^C
DsaI           11  CCGCGG               C^C[GA][CT]GG
FauI          116  CCCGC                CCCGC(4/6)
FokI           30  CATCC                GGATG(9/13)
HhaI           53  GCGC                 GCG^C
HinP1I         53  GCGC                 G^CGC
HphI          194  TCACC                GGTGA(8/7)
KspI           11  CCGCGG               CCGC^GG
MaeIII        193  GTCAC                ^GT[ACGT]AC
MboII         231  GAAGA                GAAGA(8/7)
MspA1I         11  CCGCGG               C[AC]G^C[GT]G
MvnI           12  CGCG                 CG^CG
RsaI          249  GTAC                 GT^AC
SacII          11  CCGCGG               CCGC^GG
SfaNI          61  GCATC                GCATC(5/9)
Tsp45I        193  GTCAC                ^GT[GC]AC
XcmI          226  CCAGAGAAGAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

BbvI           16  GCAGC                GCAGC(8/12)
               98  GCTGC               
BmyI          131  GTGCCC               G[AGT]GC[ACT]^C
              168  GTGCTC              
BsaJI          11  CCGCGG               C^C[ACGT][ACGT]GG
               80  CCTCGG              
Bsp1286I      131  GTGCCC               G[AGT]GC[ACT]^C
              168  GTGCTC              
BsrDI          36  CATTGC               GCAATG(2/0)
              111  CATTGC              
TseI           16  GCAGC                G^C[AT]GC
               98  GCTGC               
TspRI         122  CACTG                CA[GC]TG[ACGT][ACGT]^
              159  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

AciI        Fnu4HI      ItaI        MnlI        MwoI