##################################
# NO CUT ENZYMES (  189 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BseAI       BseRI       BsiEI       
BsiWI       BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DpnII       DraI        DraIII      DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HgaI        
Hin1I       HincII      HindII      HpaI        HphI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MamI        MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        Tru9I       Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AfaI          102  GTAC                 GT^AC
Aor51HI       203  AGCGCT               AGC^GCT
AspHI         238  GAGCTC               G[AT]GC[AT]^C
AvaII          59  GGACC                G^G[AT]CC
BsaWI         143  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       238  GAGCTC               G[AT]GC[AT]^C
BsrBI         376  GAGCGG               CCGCTC(-3/-3)
BstAPI        252  GCAGCTGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I         200  GCAAGC               GC[ACGT]^[ACGT]GC
CfoI          204  GCGC                 GCG^C
DrdI          372  GACTGAGCGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      203  AGCGCT               AGC^GCT
HaeII         203  AGCGCT               [GA]GCGC^[CT]
HapII         144  CCGG                 C^CGG
HhaI          204  GCGC                 GCG^C
HinP1I        204  GCGC                 G^CGC
HindIII       159  AAGCTT               A^AGCTT
HinfI         117  GATTC                G^A[ACGT]TC
HpaII         144  CCGG                 C^CGG
MboII         114  GAAGA                GAAGA(8/7)
MspA1I        253  CAGCTG               C[AC]G^C[GT]G
MspI          144  CCGG                 C^CGG
PpuMI          58  AGGACCC              [GA]G^G[AT]CC[CT]
PstI          306  CTGCAG               CTGCA^G
PvuII         253  CAGCTG               CAG^CTG
RsaI          102  GTAC                 GT^AC
SacI          238  GAGCTC               GAGCT^C
SfcI          306  CTGCAG               C^T[GA][CT]AG
SmlI           97  CTCAAG               C^T[CT][GA]AG
TfiI          117  GATTC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

BsgI          224  GTGCAG               GTGCAG(16/14)
              247  GTGCAG              
BsrI           12  ACTGG                ACTGG(1/-1)
              334  ACTGG               
DraII          58  AGGACCC              [GA]G^G[ACGT]CC[CT]
              322  GGGGCCT             
EcoO109I       58  AGGACCC              [GA]G^G[ACGT]CC[CT]
              322  GGGGCCT             
EcoT14I        63  CCAAGG               C^C[AT][AT]GG
              125  CCAAGG              
FauI          121  CCCGC                CCCGC(4/6)
              197  CCCGC               
MaeIII          9  GTCAC                ^GT[ACGT]AC
              381  GTCAC               
StyI           63  CCAAGG               C^C[AT][AT]GG
              125  CCAAGG              
Tsp45I          9  GTCAC                ^GT[GC]AC
              381  GTCAC               
TspRI          11  CACTG                CA[GC]TG[ACGT][ACGT]^
               69  CAGTG               
XcmI           63  CCAAGGCAGTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              232  CCAAAGGAGCTCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AciI        AluI        AlwNI       BanII       BbvI        
BmyI        BsaJI       BsiYI       BslI        Bsp1286I    
BssKI       BstF5I      BstNI       Cfr13I      DdeI        
EcoRII      Fnu4HI      FokI        HaeIII      ItaI        
MnlI        MvaI        MwoI        NlaIV       Sau96I      
ScrFI       SfaNI       TseI