##################################
# NO CUT ENZYMES (  193 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BlnI        BpmI        
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstF5I      
BstPI       BstXI       BstZ17I     Bsu36I      CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MamI        MfeI        MluI        
MroI        MseI        MslI        MspA1I      MspI        
MunI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NlaIV       NotI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        Tru9I       Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccII         144  CGCG                 CG^CG
AciI           14  CCGC                 CCGC(-3/-1)
AvaII         261  GGACC                G^G[AT]CC
BalI          209  TGGCCA               TGG^CCA
BanII          61  GGGCTC               G[GA]GC[CT]^C
BbsI          291  GAAGAC               GAAGAC(2/6)
BlpI           67  GCTCAGC              GC^T[ACGT]AGC
BmyI           61  GGGCTC               G[AGT]GC[ACT]^C
Bpu1102I       67  GCTCAGC              GC^T[ACGT]AGC
BpuAI         291  GAAGAC               GAAGAC(2/6)
BsgI          133  GTGCAG               GTGCAG(16/14)
BsmAI           7  GTCTC                GTCTC(1/5)
BsmFI         176  GTCCC                GGGAC(10/14)
Bsp1286I       61  GGGCTC               G[AGT]GC[ACT]^C
BspMI          97  ACCTGC               ACCTGC(4/8)
BsrI           91  CCAGT                ACTGG(1/-1)
BssKI         267  CCTGG                ^CC[ACGT]GG
BstAPI         79  GCAATCAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         267  CCTGG                CC^[AT]GG
BstUI         144  CGCG                 CG^CG
CelII          67  GCTCAGC              GC^T[ACGT]AGC
Cfr13I        261  GGACC                G^G[ACGT]CC
DsaI          257  CCATGG               C^C[GA][CT]GG
EaeI          209  TGGCCA               [CT]^GGCC[GA]
EcoRII        267  CCTGG                ^CC[AT]GG
HaeIII        210  GGCC                 GG^CC
MaeIII         94  GTCAC                ^GT[ACGT]AC
MluNI         209  TGGCCA               TGG^CCA
MnlI          138  GAGG                 CCTC(7/6)
MscI          209  TGGCCA               TGG^CCA
MvaI          267  CCTGG                CC^[AT]GG
MvnI          144  CGCG                 CG^CG
NcoI          257  CCATGG               C^CATGG
NlaIII        258  CATG                 CATG^
NruI          143  TCGCGA               TCG^CGA
Sau96I        261  GGACC                G^G[ACGT]CC
ScrFI         267  CCTGG                CC^[ACGT]GG
SfaNI          76  GATGC                GCATC(5/9)
SfcI          150  CTATAG               C^T[GA][CT]AG
Tsp45I         94  GTCAC                ^GT[GC]AC
TspRI         131  CAGTG                CA[GC]TG[ACGT][ACGT]^
XcmI          257  CCATGGACCACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AluI          148  AGCT                 AG^CT
              186  AGCT                
BglII         125  AGATCT               A^GATCT
              218  AGATCT              
BstYI         125  AGATCT               [GA]^GATC[CT]
              218  AGATCT              
DdeI           68  CTCAG                C^T[ACGT]AG
              129  CTCAG               
HinfI         158  GAATC                G^A[ACGT]TC
              253  GATTC               
MflI          125  AGATCT               [GA]^GATC[CT]
              218  AGATCT              
MwoI           63  GCTCGCTCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               79  GCAATCAATGC         
TfiI          158  GAATC                G^A[AT]TC
              253  GATTC               
XhoII         125  AGATCT               [GA]^GATC[CT]
              218  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

BbvI        BsaJI       Cac8I       DpnII       EcoT14I     
Fnu4HI      HphI        ItaI        MboI        MboII       
NdeII       Sau3AI      StyI        TseI