##################################
# NO CUT ENZYMES (  200 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BseRI       
BsiEI       BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DdeI        DraI        DraII       DraIII      
DrdI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HinfI       HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MamI        MboII       MfeI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        TfiI        Tsp45I      
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AciI          267  GCGG                 CCGC(-3/-1)
AspHI         228  GTGCTC               G[AT]GC[AT]^C
AvaII         292  GGTCC                G^G[AT]CC
BamHI          52  GGATCC               G^GATCC
BcnI          295  CCGGG                CC^[GC]GG
BmyI          228  GTGCTC               G[AGT]GC[ACT]^C
BsaI            6  GGTCTC               GGTCTC(1/5)
BsgI          159  CTGCAC               GTGCAG(16/14)
BsiHKAI       228  GTGCTC               G[AT]GC[AT]^C
BsiYI         257  CCAAGAAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          257  CCAAGAAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I      228  GTGCTC               G[AGT]GC[ACT]^C
BspHI         197  TCATGA               T^CATGA
BstAPI        221  GCAGCGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        183  CATCC                GGATG(2/0)
BstYI          52  GGATCC               [GA]^GATC[CT]
DpnII          53  GATC                 ^GATC
DsaI           11  CCGTGG               C^C[GA][CT]GG
EcoT14I        48  CCTTGG               C^C[AT][AT]GG
FokI          183  CATCC                GGATG(9/13)
HaeIII         60  GGCC                 GG^CC
HapII         295  CCGG                 C^CGG
HindIII       103  AAGCTT               A^AGCTT
HpaII         295  CCGG                 C^CGG
HphI          254  TCACC                GGTGA(8/7)
MboI           53  GATC                 ^GATC
MflI           52  GGATCC               [GA]^GATC[CT]
MseI           91  TTAA                 T^TAA
MspI          295  CCGG                 C^CGG
MwoI          221  GCAGCGAGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          295  CCGGG                CC^[GC]GG
NdeII          53  GATC                 ^GATC
NlaIV          52  GGATCC               GG[ACGT]^[ACGT]CC
RcaI          197  TCATGA               T^CATGA
Sau3AI         53  GATC                 ^GATC
StyI           48  CCTTGG               C^C[AT][AT]GG
Tru9I          91  TTAA                 T^TAA
XhoII          52  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AlwI           53  GATCC                GGATC(4/5)
               52  GGATC               
BsmAI          82  GAGAC                GTCTC(1/5)
                7  GTCTC               
BstNI          57  CCAGG                CC^[AT]GG
              238  CCAGG               
Cfr13I         60  GGCCC                G^G[ACGT]CC
              292  GGTCC               
EcoRII         57  CCAGG                ^CC[AT]GG
              238  CCAGG               
MvaI           57  CCAGG                CC^[AT]GG
              238  CCAGG               
Sau96I         60  GGCCC                G^G[ACGT]CC
              292  GGTCC               
SfaNI          76  GATGC                GCATC(5/9)
              182  GCATC               
TspRI         110  CACTG                CA[GC]TG[ACGT][ACGT]^
              288  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AluI        BbvI        BsaJI       BsrI        BssKI       
Fnu4HI      ItaI        MnlI        NlaIII      ScrFI       
TseI