##################################
# NO CUT ENZYMES ( 204 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DdeI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoT22I FauI FbaI
FokI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MaeII MaeIII
MamI MfeI MflI MluI MnlI
MroI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tsp45I Tsp509I
Tth111I TthHB8I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AciI 11 CCGC CCGC(-3/-1)
AlwNI 74 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 264 GGACC G^G[AT]CC
BanII 61 GGGCTC G[GA]GC[CT]^C
BfaI 136 CTAG C^TAG
BmyI 61 GGGCTC G[AGT]GC[ACT]^C
BsaI 6 GGTCTC GGTCTC(1/5)
BsgI 241 GTGCAG GTGCAG(16/14)
BsmAI 7 GTCTC GTCTC(1/5)
Bsp1286I 61 GGGCTC G[AGT]GC[ACT]^C
BspMI 91 ACCTGC ACCTGC(4/8)
DpnII 186 GATC ^GATC
EcoRV 211 GATATC GAT^ATC
EcoT14I 206 CCAAGG C^C[AT][AT]GG
HphI 155 TCACC GGTGA(8/7)
MaeI 136 CTAG C^TAG
MboI 186 GATC ^GATC
MseI 101 TTAA T^TAA
MslI 17 CACTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 41 CAGCTG C[AC]G^C[GT]G
NdeII 186 GATC ^GATC
NlaIII 252 CATG CATG^
PvuII 41 CAGCTG CAG^CTG
Sau3AI 186 GATC ^GATC
SfcI 144 CTACAG C^T[GA][CT]AG
StyI 206 CCAAGG C^C[AT][AT]GG
Tru9I 101 TTAA T^TAA
TspRI 159 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BalI 107 TGGCCA TGG^CCA
203 TGGCCA
BsmFI 82 GTCCC GGGAC(10/14)
170 GTCCC
BsrI 201 ACTGG ACTGG(1/-1)
158 CCAGT
BssKI 57 CCAGG ^CC[ACGT]GG
105 CCTGG
BstNI 57 CCAGG CC^[AT]GG
105 CCTGG
Cac8I 63 GCTCGC GC[ACGT]^[ACGT]GC
72 GCCAGC
Cfr13I 70 GGGCC G^G[ACGT]CC
264 GGACC
EaeI 107 TGGCCA [CT]^GGCC[GA]
203 TGGCCA
EcoRII 57 CCAGG ^CC[AT]GG
105 CCTGG
HinfI 152 GAATC G^A[ACGT]TC
247 GATTC
MluNI 107 TGGCCA TGG^CCA
203 TGGCCA
MscI 107 TGGCCA TGG^CCA
203 TGGCCA
MvaI 57 CCAGG CC^[AT]GG
105 CCTGG
MwoI 63 GCTCGCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
67 GCTGGGCCAGC
Sau96I 70 GGGCC G^G[ACGT]CC
264 GGACC
ScrFI 57 CCAGG CC^[ACGT]GG
105 CCTGG
TfiI 152 GAATC G^A[AT]TC
247 GATTC
XcmI 57 CCAGGGGCTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
251 CCATGAAGTATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
#########################################
AluI BbvI BsaJI Fnu4HI HaeIII
ItaI MboII TseI