Restriction map for
pF1KB6304
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BclI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MaeII MaeIII
MamI MfeI MflI MluI MluNI
MroI MscI MslI MspA1I MunI
MvnI MwoI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI TaqI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AfaI 383 GTAC GT^AC
BciVI 324 GGATAC GTATCC(6/5)
BcnI 138 CCGGG CC^[GC]GG
BsaJI 91 CCACGG C^C[ACGT][ACGT]GG
BsmBI 743 CGTCTC CGTCTC(1/5)
BsmFI 140 GGGAC GGGAC(10/14)
BsmI 285 GAATGC GAATGC(1/-1)
BspMI 27 GCAGGT ACCTGC(4/8)
BstXI 614 CCAGAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 333 GCTAGC GC[ACGT]^[ACGT]GC
DdeI 648 CTTAG C^T[ACGT]AG
DrdI 142 GACAGGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 91 CCACGG C^C[GA][CT]GG
EarI 812 GAAGAG CTCTTC(1/4)
EcoRI 571 GAATTC G^AATTC
FauI 250 CCCGC CCCGC(4/6)
HapII 138 CCGG C^CGG
HincII 295 GTTGAC GT[CT]^[GA]AC
HindII 295 GTTGAC GT[CT]^[GA]AC
HpaII 138 CCGG C^CGG
Ksp632I 812 GAAGAG CTCTTC(1/4)
MspI 138 CCGG C^CGG
NciI 138 CCGGG CC^[GC]GG
NheI 333 GCTAGC G^CTAGC
RsaI 383 GTAC GT^AC
SexAI 155 ACCTGGT A^CC[AT]GGT
SmlI 752 CTCAAG C^T[CT][GA]AG
SspI 51 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AluI 336 AGCT AG^CT
798 AGCT
AvaII 95 GGACC G^G[AT]CC
135 GGTCC
BbvI 34 GCTGC GCAGC(8/12)
592 GCTGC
BseRI 13 GAGGAG GAGGAG(10/8)
598 GAGGAG
BsiYI 138 CCGGGACAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
222 CCTTCTCATGG
BslI 138 CCGGGACAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
222 CCTTCTCATGG
BsmAI 636 GTCTC GTCTC(1/5)
744 GTCTC
BstNI 156 CCTGG CC^[AT]GG
275 CCAGG
DpnII 61 GATC ^GATC
373 GATC
EcoRII 156 CCTGG ^CC[AT]GG
275 CCAGG
HaeIII 309 GGCC GG^CC
516 GGCC
HphI 84 GGTGA GGTGA(8/7)
114 TCACC
MboI 61 GATC ^GATC
373 GATC
MvaI 156 CCTGG CC^[AT]GG
275 CCAGG
NdeII 61 GATC ^GATC
373 GATC
PleI 196 GAGTC GAGTC(4/5)
715 GAGTC
PstI 350 CTGCAG CTGCA^G
593 CTGCAG
Sau3AI 61 GATC ^GATC
373 GATC
SfaNI 625 GATGC GCATC(5/9)
498 GCATC
TfiI 584 GAATC G^A[AT]TC
738 GAATC
TseI 34 GCTGC G^C[AT]GC
592 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AccI AciI AcsI ApoI BfaI
BsrI BssKI BstF5I Cfr13I Fnu4HI
FokI HinfI ItaI MaeI MboII
MnlI MseI NlaIII NlaIV Sau96I
ScrFI SfcI Tru9I Tsp509I TspRI