Restriction map for
pF1KE0514
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbeI BciVI BclI BfaI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsgI BsiWI
BsmAI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII Cfr10I ClaI CpoI
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MaeIII MamI MfeI MluI
MluNI MroI MscI MslI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tsp45I Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcyI 451 GGCGTC G[GA]^CG[CT]C
AfaI 186 GTAC GT^AC
Alw44I 376 GTGCAC G^TGCAC
ApaLI 376 GTGCAC G^TGCAC
AvaI 366 CCCGAG C^[CT]CG[GA]G
BanI 64 GGCACC G^G[CT][GA]CC
BbrPI 319 CACGTG CAC^GTG
BbsI 454 GTCTTC GAAGAC(2/6)
BbvCI 483 CCTCAGC CCTCAGC(-5/-2)
BcgI 368 CGAGGATTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 239 CCCGG CC^[GC]GG
BglII 98 AGATCT A^GATCT
BpuAI 454 GTCTTC GAAGAC(2/6)
BsaAI 319 CACGTG [CT]AC^GT[GA]
BsaHI 451 GGCGTC G[GA]^CG[CT]C
BsiEI 552 CGACCG CG[GA][CT]^CG
BsoBI 366 CCCGAG C^[CT]CG[GA]G
CfoI 360 GCGC GCG^C
DraI 544 TTTAAA TTT^AAA
DraIII 319 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 607 GAAGAG CTCTTC(1/4)
EcoT14I 658 CCAAGG C^C[AT][AT]GG
FauI 129 GCGGG CCCGC(4/6)
HapII 240 CCGG C^CGG
HgaI 452 GCGTC GACGC(5/10)
HhaI 360 GCGC GCG^C
Hin1I 451 GGCGTC G[GA]^CG[CT]C
HinP1I 360 GCGC G^CGC
HpaII 240 CCGG C^CGG
Ksp632I 607 GAAGAG CTCTTC(1/4)
MaeII 320 ACGT A^CGT
MspI 240 CCGG C^CGG
NciI 239 CCCGG CC^[GC]GG
NlaIII 204 CATG CATG^
PmaCI 319 CACGTG CAC^GTG
PmlI 319 CACGTG CAC^GTG
PpuMI 73 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 447 GACAGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 186 GTAC GT^AC
SfaNI 307 GATGC GCATC(5/9)
StyI 658 CCAAGG C^C[AT][AT]GG
TaqI 551 TCGA T^CGA
Tsp509I 14 AATT ^AATT
TthHB8I 551 TCGA T^CGA
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AlwNI 432 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
499 CAGGAGCTG
ApaI 277 GGGCCC GGGCC^C
466 GGGCCC
BseRI 412 CTCCTC GAGGAG(10/8)
628 GAGGAG
BsrI 404 ACTGG ACTGG(1/-1)
675 CCAGT
HinfI 235 GACTC G^A[ACGT]TC
523 GACTC
HphI 120 GGTGA GGTGA(8/7)
251 TCACC
MseI 221 TTAA T^TAA
545 TTAA
PflMI 318 CCACGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
498 CCAGGAGCTGG
PleI 235 GACTC GAGTC(4/5)
523 GACTC
PvuII 136 CAGCTG CAG^CTG
163 CAGCTG
SmlI 69 CTTGAG C^T[CT][GA]AG
208 CTCAAG
Tru9I 221 TTAA T^TAA
545 TTAA
Van91I 318 CCACGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
498 CCAGGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanII BbvI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII ItaI MboI MboII
MflI MnlI MspA1I MvaI MwoI
NdeII NlaIV Sau3AI Sau96I ScrFI
TseI TspRI XhoII