Restriction map for
pF1KB7867
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BalI BbvCI BciVI BclI BfrI
BglII BlnI BsaBI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraIII Eam1105I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfuI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 1630 GACGTC GACGT^C
AflIII 119 ACACGT A^C[GA][CT]GT
Aor51HI 1232 AGCGCT AGC^GCT
ApaI 681 GGGCCC GGGCC^C
AviII 1226 TGCGCA TGC^GCA
BamHI 59 GGATCC G^GATCC
BbsI 28 GTCTTC GAAGAC(2/6)
BpuAI 28 GTCTTC GAAGAC(2/6)
BsaI 1246 GAGACC GGTCTC(1/5)
BsaWI 129 ACCGGA [AT]^CCGG[AT]
BsgI 241 GTGCAG GTGCAG(16/14)
BsiEI 1343 CGGCCG CG[GA][CT]^CG
BsrI 384 CCAGT ACTGG(1/-1)
BstXI 1307 CCACCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 59 GGATCC [GA]^GATC[CT]
DraI 928 TTTAAA TTT^AAA
DrdI 1488 GACACCGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1251 CCACGG C^C[GA][CT]GG
EagI 1343 CGGCCG C^GGCCG
EarI 210 GAAGAG CTCTTC(1/4)
EclXI 1343 CGGCCG C^GGCCG
Eco47III 1232 AGCGCT AGC^GCT
Eco52I 1343 CGGCCG C^GGCCG
EcoRV 203 GATATC GAT^ATC
EcoT14I 1052 CCAAGG C^C[AT][AT]GG
FspI 1226 TGCGCA TGC^GCA
Ksp632I 210 GAAGAG CTCTTC(1/4)
MflI 59 GGATCC [GA]^GATC[CT]
MseI 929 TTAA T^TAA
NspI 818 GCATGC [GA]CATG^[CT]
PflMI 10 CCAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1112 GGGACCC [GA]G^G[AT]CC[CT]
SacI 361 GAGCTC GAGCT^C
ScaI 446 AGTACT AGT^ACT
SgrAI 1490 CACCGGCG C[GA]^CCGG[CT]G
SphI 818 GCATGC GCATG^C
StuI 1058 AGGCCT AGG^CCT
StyI 1052 CCAAGG C^C[AT][AT]GG
TaqI 47 TCGA T^CGA
TfiI 1212 GAATC G^A[AT]TC
Tru9I 929 TTAA T^TAA
Tsp509I 1620 AATT ^AATT
TspRI 300 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 47 TCGA T^CGA
Van91I 10 CCAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 59 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 1072 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1328 CAGCCGCTG
AvaI 1378 CCCGAG C^[CT]CG[GA]G
1483 CCCGAG
BbeI 537 GGCGCC GGCGC^C
966 GGCGCC
BbrPI 120 CACGTG CAC^GTG
1222 CACGTG
BcgI 1098 CGACCTGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
782 GCATCGTGATCG
BfaI 235 CTAG C^TAG
1393 CTAG
BglI 643 GCCGGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1313 GCCTGGGGGGC
BlpI 1333 GCTGAGC GC^T[ACGT]AGC
1587 GCTGAGC
Bpu1102I 1333 GCTGAGC GC^T[ACGT]AGC
1587 GCTGAGC
BsmAI 1246 GAGAC GTCTC(1/5)
1486 GAGAC
BsmFI 1112 GGGAC GGGAC(10/14)
1044 GTCCC
BsoBI 1378 CCCGAG C^[CT]CG[GA]G
1483 CCCGAG
BsrBI 406 GAGCGG CCGCTC(-3/-3)
438 GAGCGG
BssHII 329 GCGCGC G^CGCGC
1288 GCGCGC
BstAPI 1071 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1229 GCAAGCGCTGC
CelII 1333 GCTGAGC GC^T[ACGT]AGC
1587 GCTGAGC
KasI 537 GGCGCC G^GCGCC
966 GGCGCC
MaeI 235 CTAG C^TAG
1393 CTAG
NaeI 702 GCCGGC GCC^GGC
1027 GCCGGC
NarI 537 GGCGCC GG^CGCC
966 GGCGCC
NgoMIV 702 GCCGGC G^CCGGC
1027 GCCGGC
NlaIII 807 CATG CATG^
819 CATG
PmaCI 120 CACGTG CAC^GTG
1222 CACGTG
PmlI 120 CACGTG CAC^GTG
1222 CACGTG
PvuII 11 CAGCTG CAG^CTG
193 CAGCTG
SfoI 537 GGCGCC GGC^GCC
966 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaAI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI Bsp1286I BspMI BsrFI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TseI Tsp45I
XcmI