Restriction map for
pF1KE0218
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BbvI BciVI BclI BfaI
BfrI BglI BglII BlnI BmyI
BpmI BpuAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV FauI FbaI Fnu4HI
FseI FspI HaeII Hin1I HindIII
HpaI ItaI KasI KpnI Ksp632I
KspI MaeI MamI MfeI MflI
MluI MluNI MroI MscI MspA1I
MunI NaeI NarI NdeI NgoMIV
NheI NlaIV NotI NruI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SpeI SphI Sse8387I SspBI
StuI SwaI TseI Tsp509I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 19 GTAGAC GT^[AC][GT]AC
Asp700I 492 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 347 GCATTTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 54 CCGGG CC^[GC]GG
BlpI 105 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 105 GCTGAGC GC^T[ACGT]AGC
BsaAI 16 TACGTA [CT]AC^GT[GA]
BsgI 373 CTGCAC GTGCAG(16/14)
BsiYI 54 CCGGGAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 54 CCGGGAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrDI 503 GCAATG GCAATG(2/0)
BsrI 290 ACTGG ACTGG(1/-1)
BstNI 150 CCTGG CC^[AT]GG
CelII 105 GCTGAGC GC^T[ACGT]AGC
CfoI 130 GCGC GCG^C
DdeI 106 CTGAG C^T[ACGT]AG
DpnII 144 GATC ^GATC
DsaI 92 CCATGG C^C[GA][CT]GG
EcoRII 150 CCTGG ^CC[AT]GG
EcoT22I 396 ATGCAT ATGCA^T
HapII 54 CCGG C^CGG
HgaI 127 GACGC GACGC(5/10)
HhaI 130 GCGC GCG^C
HinP1I 130 GCGC G^CGC
HincII 154 GTCAAC GT[CT]^[GA]AC
HindII 154 GTCAAC GT[CT]^[GA]AC
HpaII 54 CCGG C^CGG
HphI 266 TCACC GGTGA(8/7)
MboI 144 GATC ^GATC
MboII 205 GAAGA GAAGA(8/7)
MseI 401 TTAA T^TAA
MslI 324 CATCCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 54 CCGG C^CGG
MvaI 150 CCTGG CC^[AT]GG
NciI 54 CCGGG CC^[GC]GG
NcoI 92 CCATGG C^CATGG
NdeII 144 GATC ^GATC
NsiI 396 ATGCAT ATGCA^T
PleI 22 GACTC GAGTC(4/5)
Sau3AI 144 GATC ^GATC
SexAI 149 ACCTGGT A^CC[AT]GGT
SnaBI 16 TACGTA TAC^GTA
SspI 495 AATATT AAT^ATT
TaqI 146 TCGA T^CGA
TfiI 420 GATTC G^A[AT]TC
Tru9I 401 TTAA T^TAA
TthHB8I 146 TCGA T^CGA
XmnI 492 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 129 CGCG CG^CG
160 CGCG
AfaI 123 GTAC GT^AC
391 GTAC
AspI 148 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
184 GACGTGGTC
BsaJI 92 CCATGG C^C[ACGT][ACGT]GG
137 CCAAGG
BsmFI 30 GGGAC GGGAC(10/14)
229 GGGAC
BssKI 54 CCGGG ^CC[ACGT]GG
150 CCTGG
BstF5I 51 CATCC GGATG(2/0)
324 CATCC
BstUI 129 CGCG CG^CG
160 CGCG
Cac8I 259 GCCAGC GC[ACGT]^[ACGT]GC
302 GCTTGC
EcoT14I 92 CCATGG C^C[AT][AT]GG
137 CCAAGG
FokI 51 CATCC GGATG(9/13)
324 CATCC
HaeIII 90 GGCC GG^CC
258 GGCC
HinfI 22 GACTC G^A[ACGT]TC
420 GATTC
MaeIII 280 GTGAC ^GT[ACGT]AC
467 GTGAC
MvnI 129 CGCG CG^CG
160 CGCG
MwoI 398 GCATTAAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
503 GCAATGTCCGC
NlaIII 93 CATG CATG^
395 CATG
PflFI 148 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
184 GACGTGGTC
RsaI 123 GTAC GT^AC
391 GTAC
ScrFI 54 CCGGG CC^[ACGT]GG
150 CCTGG
StyI 92 CCATGG C^C[AT][AT]GG
137 CCAAGG
Tsp45I 280 GTGAC ^GT[GC]AC
467 GTGAC
TspRI 276 CACTG CA[GC]TG[ACGT][ACGT]^
424 CAGTG
Tth111I 148 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
184 GACGTGGTC
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# 3 OR MORE CUT ENZYMES ( 5 enzymes) #
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AciI AluI MaeII MnlI SfaNI