Restriction map for
pF1KE0354
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# NO CUT ENZYMES ( 102 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AgeI AocI Aor51HI ApoI
AscI AseI AsnI Asp718I AspI
AvaI AviII AvrII BfaI BfrI
BglI BlnI BsaBI BsaI BsaWI
BseAI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BsrI Bst1107I BstBI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eco47III Eco81I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MaeI MamI
MfeI MroI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfuI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 451 GACGTC GACGT^C
AfaI 744 GTAC GT^AC
AhdI 1609 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1269 GTGCAC G^TGCAC
ApaLI 1269 GTGCAC G^TGCAC
Asp700I 519 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1609 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 128 GGATCC G^GATCC
BbrPI 1351 CACGTG CAC^GTG
BcgI 72 GCAGCTTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 348 GTATCC GTATCC(6/5)
BclI 1598 TGATCA T^GATCA
BglII 1007 AGATCT A^GATCT
BsaAI 1351 CACGTG [CT]AC^GT[GA]
BsgI 1003 GTGCAG GTGCAG(16/14)
BsmI 1947 GAATGC GAATGC(1/-1)
BsrDI 1728 CATTGC GCAATG(2/0)
BssHII 1428 GCGCGC G^CGCGC
BstEII 21 GGTCACC G^GT[ACGT]ACC
BstPI 21 GGTCACC G^GT[ACGT]ACC
EagI 1577 CGGCCG C^GGCCG
Eam1105I 1609 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 754 GAAGAG CTCTTC(1/4)
EclXI 1577 CGGCCG C^GGCCG
Eco52I 1577 CGGCCG C^GGCCG
EcoO65I 21 GGTCACC G^GT[ACGT]ACC
FbaI 1598 TGATCA T^GATCA
Ksp632I 754 GAAGAG CTCTTC(1/4)
MluI 776 ACGCGT A^CGCGT
NaeI 1525 GCCGGC GCC^GGC
NcoI 850 CCATGG C^CATGG
NgoMIV 1525 GCCGGC G^CCGGC
PmaCI 1351 CACGTG CAC^GTG
PmlI 1351 CACGTG CAC^GTG
PpuMI 1783 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 286 GACGTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 744 GTAC GT^AC
SexAI 1499 ACCTGGT A^CC[AT]GGT
SgrAI 1524 CGCCGGCG C[GA]^CCGG[CT]G
Tsp509I 102 AATT ^AATT
XmnI 519 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AlwI 129 GATCC GGATC(4/5)
128 GGATC
BalI 695 TGGCCA TGG^CCA
2144 TGGCCA
BbvCI 1446 CCTCAGC CCTCAGC(-5/-2)
1932 GCTGAGG
BlpI 247 GCTGAGC GC^T[ACGT]AGC
1017 GCTGAGC
Bpu1102I 247 GCTGAGC GC^T[ACGT]AGC
1017 GCTGAGC
BsiEI 1434 CGACCG CG[GA][CT]^CG
1577 CGGCCG
BsmAI 651 GAGAC GTCTC(1/5)
1617 GTCTC
BsrBI 1437 CCGCTC CCGCTC(-3/-3)
2032 GAGCGG
BsrFI 1301 ACCGGC [GA]^CCGG[CT]
1525 GCCGGC
BssSI 34 CTCGTG CACGAG(-5/-1)
709 CTCGTG
BstYI 128 GGATCC [GA]^GATC[CT]
1007 AGATCT
CelII 247 GCTGAGC GC^T[ACGT]AGC
1017 GCTGAGC
Cfr10I 1301 ACCGGC [GA]^CCGG[CT]
1525 GCCGGC
DraIII 799 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
915 CACCGAGTG
HgaI 410 GCGTC GACGC(5/10)
1485 GCGTC
MflI 128 GGATCC [GA]^GATC[CT]
1007 AGATCT
MluNI 695 TGGCCA TGG^CCA
2144 TGGCCA
MscI 695 TGGCCA TGG^CCA
2144 TGGCCA
MseI 1159 TTAA T^TAA
1886 TTAA
PleI 196 GACTC GAGTC(4/5)
703 GAGTC
StuI 548 AGGCCT AGG^CCT
1715 AGGCCT
Tru9I 1159 TTAA T^TAA
1886 TTAA
Tsp45I 22 GTCAC ^GT[GC]AC
1465 GTGAC
XcmI 26 CCATCCAGCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2085 CCAGCACCTGGATGG
XhoII 128 GGATCC [GA]^GATC[CT]
1007 AGATCT
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcyI AflIII AluI
AlwNI ApaI AspHI AvaII BanI
BanII BbeI BbsI BbvI BcnI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BspMI BssKI BstAPI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PflMI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI StyI TaqI
TfiI TseI TspRI TthHB8I Van91I