Restriction map for
pF1KB8435
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Aor51HI ApaI
AseI AsnI Asp718I AviII AvrII
BamHI BbeI BbvCI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BspLU11I
BsrBI BsrDI Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI EarI EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT14I
EcoT22I FbaI FspI HaeII Hin1I
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StyI
SwaI XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 936 GAATTT [GA]^AATT[CT]
AflIII 1326 ACACGT A^C[GA][CT]GT
AocI 547 CCTGAGG CC^T[ACGT]AGG
ApoI 936 GAATTT [GA]^AATT[CT]
AscI 1486 GGCGCGCC GG^CGCGCC
Asp700I 873 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 715 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1482 CTCGGG C^[CT]CG[GA]G
BanII 289 GAGCTC G[GA]GC[CT]^C
BbrPI 1327 CACGTG CAC^GTG
BcgI 327 CGAGGCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 347 GTATCC GTATCC(6/5)
BcnI 1109 CCCGG CC^[GC]GG
BfaI 478 CTAG C^TAG
BsaAI 1327 CACGTG [CT]AC^GT[GA]
BsmI 207 GCATTC GAATGC(1/-1)
BsoBI 1482 CTCGGG C^[CT]CG[GA]G
Bsp1407I 611 TGTACA T^GTACA
BsrGI 611 TGTACA T^GTACA
BssHII 1487 GCGCGC G^CGCGC
BssSI 622 CTCGTG CACGAG(-5/-1)
BstAPI 1461 GCAGAACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 12 GGTGACC G^GT[ACGT]ACC
BstPI 12 GGTGACC G^GT[ACGT]ACC
Bsu36I 547 CCTGAGG CC^T[ACGT]AGG
Eco81I 547 CCTGAGG CC^T[ACGT]AGG
EcoO65I 12 GGTGACC G^GT[ACGT]ACC
FseI 1399 GGCCGGCC GGCCGG^CC
HincII 1156 GTCAAC GT[CT]^[GA]AC
HindII 1156 GTCAAC GT[CT]^[GA]AC
MaeI 478 CTAG C^TAG
MseI 1127 TTAA T^TAA
MspA1I 1501 CCGCTG C[AC]G^C[GT]G
NaeI 1400 GCCGGC GCC^GGC
NciI 1109 CCCGG CC^[GC]GG
NgoMIV 1400 GCCGGC G^CCGGC
NspI 595 GCATGT [GA]CATG^[CT]
PflFI 715 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1327 CACGTG CAC^GTG
PmlI 1327 CACGTG CAC^GTG
PpuMI 551 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 289 GAGCTC GAGCT^C
ScaI 317 AGTACT AGT^ACT
SspBI 611 TGTACA T^GTACA
StuI 1175 AGGCCT AGG^CCT
TfiI 1262 GATTC G^A[AT]TC
Tru9I 1127 TTAA T^TAA
Tsp509I 937 AATT ^AATT
Tth111I 715 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 873 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AhdI 86 GACGCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
694 GACTTCCCGTC
Alw44I 1330 GTGCAC G^TGCAC
1468 GTGCAC
ApaLI 1330 GTGCAC G^TGCAC
1468 GTGCAC
AspEI 86 GACGCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
694 GACTTCCCGTC
BanI 685 GGTGCC G^G[CT][GA]CC
1439 GGCACC
BglI 787 GCCCATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1445 GCCGCGACGGC
BsaI 404 GAGACC GGTCTC(1/5)
1426 GAGACC
BseRI 244 GAGGAG GAGGAG(10/8)
1423 GAGGAG
BsrFI 106 ACCGGC [GA]^CCGG[CT]
1400 GCCGGC
BstXI 634 CCACCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
917 CCACCATGCTGG
BstYI 188 GGATCT [GA]^GATC[CT]
356 AGATCC
Cfr10I 106 ACCGGC [GA]^CCGG[CT]
1400 GCCGGC
DraII 551 AGGTCCT [GA]G^G[ACGT]CC[CT]
794 AGGCCCT
Eam1105I 86 GACGCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
694 GACTTCCCGTC
EcoO109I 551 AGGTCCT [GA]G^G[ACGT]CC[CT]
794 AGGCCCT
MflI 188 GGATCT [GA]^GATC[CT]
356 AGATCC
PflMI 165 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
255 CCACTACCTGG
PleI 980 GAGTC GAGTC(4/5)
1060 GAGTC
PstI 452 CTGCAG CTGCA^G
1504 CTGCAG
SexAI 260 ACCTGGT A^CC[AT]GGT
1235 ACCTGGT
SmlI 409 CTCAAG C^T[CT][GA]AG
898 CTCAAG
Van91I 165 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
255 CCACTACCTGG
XhoII 188 GGATCT [GA]^GATC[CT]
356 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BalI BbsI
BbvI BmyI BpmI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TseI Tsp45I TspRI TthHB8I