Restriction map for
pF1KB7234
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AclI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AviII AvrII BalI BbeI BbrPI
BfrI BglI BglII BlnI BsaBI
BsaI BsiWI BsmI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FseI FspI HindIII HpaI
KasI MamI MfeI MluI MluNI
MscI MseI MunI NaeI NarI
NcoI NgoMIV NheI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SalI SapI SbfI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tru9I Tth111I TthHB8I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 954 GACGTC GACGT^C
Acc65I 473 GGTACC G^GTACC
AccI 922 GTCTAC GT^[AC][GT]AC
AccIII 622 TCCGGA T^CCGGA
AcsI 1153 GAATTC [GA]^AATT[CT]
AflIII 730 ACGTGT A^C[GA][CT]GT
ApaI 8 GGGCCC GGGCC^C
ApoI 1153 GAATTC [GA]^AATT[CT]
Asp718I 473 GGTACC G^GTACC
BamHI 263 GGATCC G^GATCC
BbsI 325 GAAGAC GAAGAC(2/6)
BbvCI 103 GCTGAGG CCTCAGC(-5/-2)
BcgI 927 CGACCCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 844 GTATCC GTATCC(6/5)
BclI 1007 TGATCA T^GATCA
BlpI 426 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 426 GCTCAGC GC^T[ACGT]AGC
BpuAI 325 GAAGAC GAAGAC(2/6)
BsaWI 622 TCCGGA [AT]^CCGG[AT]
BseAI 622 TCCGGA T^CCGGA
BseRI 238 GAGGAG GAGGAG(10/8)
BsiEI 47 CGGCCG CG[GA][CT]^CG
BsmBI 820 GAGACG CGTCTC(1/5)
BspEI 622 TCCGGA T^CCGGA
BspMI 127 ACCTGC ACCTGC(4/8)
BstAPI 642 GCAGTACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 426 GCTCAGC GC^T[ACGT]AGC
EagI 47 CGGCCG C^GGCCG
EarI 190 CTCTTC CTCTTC(1/4)
EclXI 47 CGGCCG C^GGCCG
Eco52I 47 CGGCCG C^GGCCG
EcoRI 1153 GAATTC G^AATTC
FbaI 1007 TGATCA T^GATCA
HincII 726 GTTGAC GT[CT]^[GA]AC
HindII 726 GTTGAC GT[CT]^[GA]AC
KpnI 473 GGTACC GGTAC^C
Ksp632I 190 CTCTTC CTCTTC(1/4)
MroI 622 TCCGGA T^CCGGA
NdeI 1011 CATATG CA^TATG
NotI 46 GCGGCCGC GC^GGCCGC
PflMI 693 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 787 GAGTC GAGTC(4/5)
PvuII 429 CAGCTG CAG^CTG
SacI 424 GAGCTC GAGCT^C
ScaI 662 AGTACT AGT^ACT
SmlI 235 CTTGAG C^T[CT][GA]AG
SnaBI 910 TACGTA TAC^GTA
SpeI 360 ACTAGT A^CTAGT
Tsp509I 1154 AATT ^AATT
Van91I 693 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1071 CCACGAAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 876 GGCGTC G[GA]^CG[CT]C
954 GACGTC
AlwNI 98 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
610 CAGGCGCTG
AspHI 424 GAGCTC G[AT]GC[AT]^C
1038 GTGCTC
BanII 8 GGGCCC G[GA]GC[CT]^C
424 GAGCTC
BfaI 361 CTAG C^TAG
1021 CTAG
BpmI 286 CTGGAG CTGGAG(16/14)
531 CTGGAG
BsaAI 646 TACGTG [CT]AC^GT[GA]
910 TACGTA
BsaHI 876 GGCGTC G[GA]^CG[CT]C
954 GACGTC
BsgI 136 CTGCAC GTGCAG(16/14)
658 GTGCAG
BsiHKAI 424 GAGCTC G[AT]GC[AT]^C
1038 GTGCTC
BsmAI 713 GAGAC GTCTC(1/5)
820 GAGAC
BsmFI 479 GGGAC GGGAC(10/14)
447 GTCCC
BstYI 263 GGATCC [GA]^GATC[CT]
764 AGATCC
Hin1I 876 GGCGTC G[GA]^CG[CT]C
954 GACGTC
HphI 702 GGTGA GGTGA(8/7)
125 TCACC
MaeI 361 CTAG C^TAG
1021 CTAG
MaeIII 94 GTGAC ^GT[ACGT]AC
739 GTCAC
MflI 263 GGATCC [GA]^GATC[CT]
764 AGATCC
SfaNI 277 GATGC GCATC(5/9)
983 GCATC
TfiI 375 GAATC G^A[AT]TC
681 GATTC
Tsp45I 94 GTGAC ^GT[GC]AC
739 GTCAC
XhoII 263 GGATCC [GA]^GATC[CT]
764 AGATCC
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BanI BbvI BcnI BmyI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
KspI MaeII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
RsaI SacII Sau3AI Sau96I ScrFI
TseI TspRI