##################################
# NO CUT ENZYMES (  195 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AvaI        AvaII       AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstEII      BstF5I      BstPI       BstUI       
BstYI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      ClaI        CpoI        DpnII       
DraI        DrdI        DsaI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        KspI        MaeI        MaeII       
MaeIII      MamI        MboI        MfeI        MflI        
MluI        MroI        MseI        MslI        MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NotI        NruI        NsiI        NspI        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacI        SacII       SalI        Sau3AI      
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfoI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tru9I       Tsp45I      
Tsp509I     Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AciI          324  GCGG                 CCGC(-3/-1)
AhdI          223  GACCAGTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         270  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspEI         223  GACCAGTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         157  GAGCAC               G[AT]GC[AT]^C
BalI          287  TGGCCA               TGG^CCA
BanII          84  GAGCCC               G[GA]GC[CT]^C
BbsI          220  GAAGAC               GAAGAC(2/6)
BbvCI         267  CCTCAGC              CCTCAGC(-5/-2)
BglI          315  GCCATTGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         220  GAAGAC               GAAGAC(2/6)
BseRI         171  CTCCTC               GAGGAG(10/8)
BsiHKAI       157  GAGCAC               G[AT]GC[AT]^C
BstBI         151  TTCGAA               TT^CGAA
BstXI         290  CCAGTGTCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII          90  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DraIII        160  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          287  TGGCCA               [CT]^GGCC[GA]
Eam1105I      223  GACCAGTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          154  GAAGAG               CTCTTC(1/4)
EcoO109I       90  AGGCCCC              [GA]G^G[ACGT]CC[CT]
HinfI         138  GAATC                G^A[ACGT]TC
Ksp632I       154  GAAGAG               CTCTTC(1/4)
MluNI         287  TGGCCA               TGG^CCA
MscI          287  TGGCCA               TGG^CCA
MspA1I        242  CAGCTG               C[AC]G^C[GT]G
NspV          151  TTCGAA               TT^CGAA
PvuII         242  CAGCTG               CAG^CTG
SapI          154  GAAGAGC              GCTCTTC(1/4)
SfuI          151  TTCGAA               TT^CGAA
TaqI          152  TCGA                 T^CGA
TfiI          138  GAATC                G^A[AT]TC
TthHB8I       152  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

BmyI           84  GAGCCC               G[AGT]GC[ACT]^C
              157  GAGCAC              
BsaJI           8  CCCTGG               C^C[ACGT][ACGT]GG
               87  CCCAGG              
Bsp1286I       84  GAGCCC               G[AGT]GC[ACT]^C
              157  GAGCAC              
BspMI          63  GCAGGT               ACCTGC(4/8)
              192  GCAGGT              
BsrI          225  CCAGT                ACTGG(1/-1)
              290  CCAGT               
HphI           66  GGTGA                GGTGA(8/7)
              181  GGTGA               


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AluI        BbvI        BsiYI       BslI        BssKI       
BstNI       Cfr13I      DdeI        EcoRII      Fnu4HI      
HaeIII      ItaI        MboII       MnlI        MvaI        
MwoI        NlaIII      NlaIV       PstI        Sau96I      
ScrFI       SfcI        TseI        TspRI