Restriction map for
pF1KE0178
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Cac8I CelII ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRI
FseI FspI HindIII HpaI KasI
KpnI KspI MaeI MaeII MaeIII
MamI MluI MroI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI RcaI
RsrII SacII SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp45I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccI 955 GTCGAC GT^[AC][GT]AC
AcyI 511 GACGCC G[GA]^CG[CT]C
AflIII 344 ACATGT A^C[GA][CT]GT
AlwI 3 GGATC GGATC(4/5)
AocI 607 CCTGAGG CC^T[ACGT]AGG
AseI 207 ATTAAT AT^TAAT
AsnI 207 ATTAAT AT^TAAT
Asp700I 577 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 905 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 996 GGTCC G^G[AT]CC
BalI 920 TGGCCA TGG^CCA
BciVI 162 GTATCC GTATCC(6/5)
BclI 548 TGATCA T^GATCA
BpmI 275 CTGGAG CTGGAG(16/14)
BsaHI 511 GACGCC G[GA]^CG[CT]C
BseRI 696 GAGGAG GAGGAG(10/8)
BsgI 465 CTGCAC GTGCAG(16/14)
BspLU11I 344 ACATGT A^CATGT
BspMI 752 ACCTGC ACCTGC(4/8)
BsrBI 667 GAGCGG CCGCTC(-3/-3)
BsrDI 330 CATTGC GCAATG(2/0)
BsrFI 70 ACCGGC [GA]^CCGG[CT]
BstAPI 828 GCATAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 75 CCATCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 3 GGATCT [GA]^GATC[CT]
Bsu36I 607 CCTGAGG CC^T[ACGT]AGG
Cfr10I 70 ACCGGC [GA]^CCGG[CT]
Cfr13I 996 GGTCC G^G[ACGT]CC
DraI 777 TTTAAA TTT^AAA
DsaI 691 CCATGG C^C[GA][CT]GG
EarI 577 GAAGAG CTCTTC(1/4)
Eco81I 607 CCTGAGG CC^T[ACGT]AGG
EcoNI 567 CCTGTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 943 GATATC GAT^ATC
EcoT22I 826 ATGCAT ATGCA^T
FauI 156 CCCGC CCCGC(4/6)
FbaI 548 TGATCA T^GATCA
HapII 71 CCGG C^CGG
HgaI 511 GACGC GACGC(5/10)
Hin1I 511 GACGCC G[GA]^CG[CT]C
HpaII 71 CCGG C^CGG
Ksp632I 577 GAAGAG CTCTTC(1/4)
MfeI 101 CAATTG C^AATTG
MflI 3 GGATCT [GA]^GATC[CT]
MluNI 920 TGGCCA TGG^CCA
MscI 920 TGGCCA TGG^CCA
MspI 71 CCGG C^CGG
MunI 101 CAATTG C^AATTG
NcoI 691 CCATGG C^CATGG
NsiI 826 ATGCAT ATGCA^T
PflFI 905 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 442 GAGTC GAGTC(4/5)
PshBI 207 ATTAAT AT^TAAT
PvuII 121 CAGCTG CAG^CTG
SacI 278 GAGCTC GAGCT^C
SalI 955 GTCGAC G^TCGAC
Sau96I 996 GGTCC G^G[ACGT]CC
TaqI 956 TCGA T^CGA
Tth111I 905 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 956 TCGA T^CGA
XhoII 3 GGATCT [GA]^GATC[CT]
XmnI 577 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 109 AAATTT [GA]^AATT[CT]
523 AAATTT
AfaI 342 GTAC GT^AC
603 GTAC
ApoI 109 AAATTT [GA]^AATT[CT]
523 AAATTT
AspHI 278 GAGCTC G[AT]GC[AT]^C
786 GTGCTC
BanII 278 GAGCTC G[GA]GC[CT]^C
448 GAGCCC
BsaI 147 GAGACC GGTCTC(1/5)
699 GAGACC
BsiHKAI 278 GAGCTC G[AT]GC[AT]^C
786 GTGCTC
CfoI 291 GCGC GCG^C
654 GCGC
EaeI 72 CGGCCA [CT]^GGCC[GA]
920 TGGCCA
HaeII 290 GGCGCT [GA]GCGC^[CT]
653 GGCGCT
HhaI 291 GCGC GCG^C
654 GCGC
HinP1I 291 GCGC G^CGC
654 GCGC
HincII 13 GTCAAC GT[CT]^[GA]AC
955 GTCGAC
HindII 13 GTCAAC GT[CT]^[GA]AC
955 GTCGAC
MseI 208 TTAA T^TAA
778 TTAA
MslI 76 CATCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
373 CATGGGGGTG
MspA1I 121 CAGCTG C[AC]G^C[GT]G
858 CAGCGG
MwoI 828 GCATAAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
927 GCCAATAGAGC
NspI 86 GCATGT [GA]CATG^[CT]
344 ACATGT
RsaI 342 GTAC GT^AC
603 GTAC
TfiI 234 GATTC G^A[AT]TC
582 GATTC
Tru9I 208 TTAA T^TAA
778 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AlwNI BbsI BbvI
BmyI BpuAI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI DdeI DpnII EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MboI MboII MnlI MvaI
NdeII NlaIII NlaIV PflMI Sau3AI
ScrFI SfaNI StyI TseI Tsp509I
TspRI Van91I