Restriction map for
pF1KE0492
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI Alw44I AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII BalI BamHI BbeI
BbrPI BcgI BciVI BclI BcnI
BfrI BglI BglII BpmI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII EaeI EagI EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HapII HgaI
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeII
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MseI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XcmI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AhdI 259 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 296 GATCC GGATC(4/5)
Aor51HI 169 AGCGCT AGC^GCT
ApaI 41 GGGCCC GGGCC^C
AspEI 259 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 186 CCTAGG C^CTAGG
BanI 752 GGCACC G^G[CT][GA]CC
BbvCI 640 GCTGAGG CCTCAGC(-5/-2)
BfaI 187 CTAG C^TAG
BlnI 186 CCTAGG C^CTAGG
BlpI 337 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 337 GCTCAGC GC^T[ACGT]AGC
BsaI 474 GGTCTC GGTCTC(1/5)
BsmI 282 GAATGC GAATGC(1/-1)
BspHI 659 TCATGA T^CATGA
BspMI 19 GCAGGT ACCTGC(4/8)
BsrDI 455 GCAATG GCAATG(2/0)
BstXI 441 CCACAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 337 GCTCAGC GC^T[ACGT]AGC
CfoI 170 GCGC GCG^C
DrdI 466 GACCAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 12 CCACGG C^C[GA][CT]GG
Eam1105I 259 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 796 GAAGAG CTCTTC(1/4)
Eco47III 169 AGCGCT AGC^GCT
EcoNI 657 CCTCATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 286 GCGGG CCCGC(4/6)
HaeII 169 AGCGCT [GA]GCGC^[CT]
HhaI 170 GCGC GCG^C
HinP1I 170 GCGC G^CGC
HphI 35 TCACC GGTGA(8/7)
Ksp632I 796 GAAGAG CTCTTC(1/4)
MaeI 187 CTAG C^TAG
MspA1I 469 CAGCTG C[AC]G^C[GT]G
NspI 611 ACATGC [GA]CATG^[CT]
PaeR7I 303 CTCGAG C^TCGAG
PvuII 469 CAGCTG CAG^CTG
RcaI 659 TCATGA T^CATGA
SacI 828 GAGCTC GAGCT^C
SfaNI 419 GCATC GCATC(5/9)
SfcI 96 CTACAG C^T[GA][CT]AG
XhoI 303 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 23 GTAC GT^AC
837 GTAC
AlwNI 130 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
148 CAGCCCCTG
AspHI 393 GAGCAC G[AT]GC[AT]^C
828 GAGCTC
AvaI 303 CTCGAG C^[CT]CG[GA]G
324 CTCGGG
BsiHKAI 393 GAGCAC G[AT]GC[AT]^C
828 GAGCTC
BsmAI 646 GAGAC GTCTC(1/5)
475 GTCTC
BsoBI 303 CTCGAG C^[CT]CG[GA]G
324 CTCGGG
BsrI 716 ACTGG ACTGG(1/-1)
792 ACTGG
DpnII 296 GATC ^GATC
739 GATC
EcoT14I 104 CCTTGG C^C[AT][AT]GG
186 CCTAGG
MboI 296 GATC ^GATC
739 GATC
MslI 46 CATCTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
715 CACTGGCATG
MwoI 170 GCGCTCAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
490 GCTCAAAAGGC
NdeII 296 GATC ^GATC
739 GATC
PpuMI 271 AGGTCCC [GA]G^G[AT]CC[CT]
550 AGGACCC
RsaI 23 GTAC GT^AC
837 GTAC
Sau3AI 296 GATC ^GATC
739 GATC
SmlI 173 CTCAAG C^T[CT][GA]AG
303 CTCGAG
StyI 104 CCTTGG C^C[AT][AT]GG
186 CCTAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI AvaII BanII BbsI
BbvI BmyI BpuAI BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MboII MnlI
MvaI NlaIII NlaIV Sau96I ScrFI
TaqI TfiI TseI TspRI TthHB8I