Restriction map for
pF1KE0338
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BanII
BbeI BbrPI BbvCI BcgI BcnI
BfaI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
Bsp1407I BspEI BspHI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HapII HgaI Hin1I HindIII HpaI
HpaII KasI KpnI KspI MaeI
MamI MluI MluNI MroI MscI
MspI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccII 1135 CGCG CG^CG
AflII 537 CTTAAG C^TTAAG
AflIII 1035 ACATGT A^C[GA][CT]GT
AlwNI 932 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 1304 CCCGAG C^[CT]CG[GA]G
BamHI 1126 GGATCC G^GATCC
BanI 1207 GGCACC G^G[CT][GA]CC
BciVI 331 GTATCC GTATCC(6/5)
BclI 1505 TGATCA T^GATCA
BfrI 537 CTTAAG C^TTAAG
BsoBI 1304 CCCGAG C^[CT]CG[GA]G
BspDI 239 ATCGAT AT^CGAT
BspLU11I 1035 ACATGT A^CATGT
BstUI 1135 CGCG CG^CG
BstYI 1126 GGATCC [GA]^GATC[CT]
ClaI 239 ATCGAT AT^CGAT
DraII 658 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 1211 CCATGG C^C[GA][CT]GG
EcoO109I 658 GGGACCC [GA]G^G[ACGT]CC[CT]
FbaI 1505 TGATCA T^GATCA
HincII 955 GTCAAC GT[CT]^[GA]AC
HindII 955 GTCAAC GT[CT]^[GA]AC
MaeII 1320 ACGT A^CGT
MaeIII 1432 GTGAC ^GT[ACGT]AC
MfeI 1496 CAATTG C^AATTG
MflI 1126 GGATCC [GA]^GATC[CT]
MslI 480 CATCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 932 CAGCTG C[AC]G^C[GT]G
MunI 1496 CAATTG C^AATTG
MvnI 1135 CGCG CG^CG
NcoI 1211 CCATGG C^CATGG
PflMI 693 CCAGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 658 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 932 CAGCTG CAG^CTG
SapI 962 GCTCTTC GCTCTTC(1/4)
SmlI 537 CTTAAG C^T[CT][GA]AG
Tsp45I 1432 GTGAC ^GT[GC]AC
Van91I 693 CCAGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1126 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 585 GTAC GT^AC
1114 GTAC
Asp700I 69 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
890 GAATCGCTTC
BbsI 562 GAAGAC GAAGAC(2/6)
1003 GTCTTC
BpmI 57 CTGGAG CTGGAG(16/14)
124 CTGGAG
BpuAI 562 GAAGAC GAAGAC(2/6)
1003 GTCTTC
BsaI 1261 GAGACC GGTCTC(1/5)
1491 GAGACC
BsiYI 649 CCTCAGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
693 CCAGGTTGTGG
BslI 649 CCTCAGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
693 CCAGGTTGTGG
BsmI 153 GAATGC GAATGC(1/-1)
489 GAATGC
BspMI 1450 ACCTGC ACCTGC(4/8)
90 GCAGGT
BsrBI 960 CCGCTC CCGCTC(-3/-3)
1286 GAGCGG
CfoI 817 GCGC GCG^C
1136 GCGC
FauI 1133 CCCGC CCCGC(4/6)
1288 GCGGG
HhaI 817 GCGC GCG^C
1136 GCGC
HinP1I 817 GCGC G^CGC
1136 GCGC
HphI 1149 TCACC GGTGA(8/7)
1387 TCACC
PleI 3 GAGTC GAGTC(4/5)
1417 GAGTC
PstI 25 CTGCAG CTGCA^G
598 CTGCAG
RsaI 585 GTAC GT^AC
1114 GTAC
StuI 509 AGGCCT AGG^CCT
642 AGGCCT
TfiI 890 GAATC G^A[AT]TC
1466 GAATC
XmnI 69 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
890 GAATCGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BbvI BmyI BsaJI BseRI BsiHKAI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
ItaI Ksp632I MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV NspI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I XcmI