Restriction map for
pF1KE0515
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanII BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI Bsp1286I
Bsp1407I BspDI BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DpnII DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI KpnI KspI MaeII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MspA1I MunI MvnI NaeI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
Sau3AI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tru9I Tth111I TthHB8I
XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 52 enzymes) #
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AciI 54 CCGC CCGC(-3/-1)
AcsI 508 GAATTC [GA]^AATT[CT]
AcyI 50 GGCGCC G[GA]^CG[CT]C
AfaI 549 GTAC GT^AC
AflIII 293 ACATGT A^C[GA][CT]GT
AlwNI 452 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 508 GAATTC [GA]^AATT[CT]
Asp700I 504 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 78 CCCGGG C^[CT]CG[GA]G
AvaII 83 GGACC G^G[AT]CC
BanI 50 GGCGCC G^G[CT][GA]CC
BbeI 50 GGCGCC GGCGC^C
BbsI 598 GAAGAC GAAGAC(2/6)
BfaI 463 CTAG C^TAG
BglI 304 GCCAATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 449 CTCCAG CTGGAG(16/14)
BpuAI 598 GAAGAC GAAGAC(2/6)
BsaHI 50 GGCGCC G[GA]^CG[CT]C
BseRI 167 GAGGAG GAGGAG(10/8)
BsoBI 78 CCCGGG C^[CT]CG[GA]G
BspHI 158 TCATGA T^CATGA
BspLU11I 293 ACATGT A^CATGT
BsrI 40 ACTGG ACTGG(1/-1)
BstXI 347 CCAGGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EarI 475 GAAGAG CTCTTC(1/4)
EcoNI 531 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 508 GAATTC G^AATTC
Hin1I 50 GGCGCC G[GA]^CG[CT]C
HphI 483 GGTGA GGTGA(8/7)
KasI 50 GGCGCC G^GCGCC
Ksp632I 475 GAAGAG CTCTTC(1/4)
MaeI 463 CTAG C^TAG
MaeIII 28 GTGAC ^GT[ACGT]AC
NarI 50 GGCGCC GG^CGCC
NspI 293 ACATGT [GA]CATG^[CT]
PflMI 144 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 82 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 158 TCATGA T^CATGA
RsaI 549 GTAC GT^AC
SapI 475 GAAGAGC GCTCTTC(1/4)
SfaNI 172 GATGC GCATC(5/9)
SfoI 50 GGCGCC GGC^GCC
SmaI 78 CCCGGG CCC^GGG
SmlI 338 CTCAAG C^T[CT][GA]AG
Tsp45I 28 GTGAC ^GT[GC]AC
Tsp509I 509 AATT ^AATT
TspRI 187 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 144 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 344 CCACCAGGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 78 CCCGGG C^CCGGG
XmaI 78 CCCGGG C^CCGGG
XmnI 504 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
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BsgI 434 CTGCAC GTGCAG(16/14)
189 GTGCAG
BspMI 85 ACCTGC ACCTGC(4/8)
248 ACCTGC
Cac8I 89 GCGAGC GC[ACGT]^[ACGT]GC
285 GCCTGC
CfoI 51 GCGC GCG^C
141 GCGC
HaeII 50 GGCGCC [GA]GCGC^[CT]
140 AGCGCC
HhaI 51 GCGC GCG^C
141 GCGC
HinP1I 51 GCGC G^CGC
141 GCGC
MboII 475 GAAGA GAAGA(8/7)
598 GAAGA
PleI 374 GACTC GAGTC(4/5)
212 GAGTC
SfcI 184 CTACAG C^T[GA][CT]AG
315 CTACAG
TfiI 115 GAATC G^A[AT]TC
557 GAATC
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI BbvI BcnI BsaJI BsiYI
BslI BsmFI BssKI BstF5I BstNI
Cfr13I DdeI DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MnlI MspI MvaI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI TseI