##################################
# NO CUT ENZYMES (  184 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmBI       BsmFI       BsoBI       
Bsp1286I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraII       DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        Fnu4HI      
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HpaII       ItaI        
KasI        KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    StuI        StyI        
SwaI        TseI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

Acc65I        129  GGTACC               G^GTACC
AciI          792  CCGC                 CCGC(-3/-1)
AlwI          312  GGATC                GGATC(4/5)
Asp700I       808  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       129  GGTACC               G^GTACC
BfaI          611  CTAG                 C^TAG
BsaJI         210  CCAGGG               C^C[ACGT][ACGT]GG
BseRI          10  GAGGAG               GAGGAG(10/8)
BsmAI         601  GAGAC                GTCTC(1/5)
BsmI          699  GAATGC               GAATGC(1/-1)
Bsp1407I      469  TGTACA               T^GTACA
BspMI         451  GCAGGT               ACCTGC(4/8)
BsrGI         469  TGTACA               T^GTACA
BstF5I        640  CATCC                GGATG(2/0)
BstYI         312  GGATCT               [GA]^GATC[CT]
Cfr13I        168  GGGCC                G^G[ACGT]CC
DraIII        748  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRI         666  GAATTC               G^AATTC
FokI          640  CATCC                GGATG(9/13)
HincII        741  GTTAAC               GT[CT]^[GA]AC
HindII        741  GTTAAC               GT[CT]^[GA]AC
HindIII       229  AAGCTT               A^AGCTT
HpaI          741  GTTAAC               GTT^AAC
KpnI          129  GGTACC               GGTAC^C
MaeI          611  CTAG                 C^TAG
MaeII         797  ACGT                 A^CGT
MflI          312  GGATCT               [GA]^GATC[CT]
Sau96I        168  GGGCC                G^G[ACGT]CC
ScaI          241  AGTACT               AGT^ACT
SfcI          712  CTACAG               C^T[GA][CT]AG
SspBI         469  TGTACA               T^GTACA
Tsp45I        575  GTCAC                ^GT[GC]AC
XhoII         312  GGATCT               [GA]^GATC[CT]
XmnI          808  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AcsI          479  GAATTT               [GA]^AATT[CT]
              666  GAATTC              
AlwNI         323  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              811  CAGCTTCTG           
ApoI          479  GAATTT               [GA]^AATT[CT]
              666  GAATTC              
BanI          129  GGTACC               G^G[CT][GA]CC
              535  GGTGCC              
BsgI          292  GTGCAG               GTGCAG(16/14)
              449  GTGCAG              
BsrI           66  ACTGG                ACTGG(1/-1)
              284  ACTGG               
DdeI          227  CTAAG                C^T[ACGT]AG
              830  CTAAG               
HphI          682  GGTGA                GGTGA(8/7)
              747  TCACC               
MwoI          708  GCTCCTACAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              846  GCAAGTAGGGC         
NlaIII        787  CATG                 CATG^
              839  CATG                
PleI          406  GACTC                GAGTC(4/5)
              675  GACTC               
SspI          118  AATATT               AAT^ATT
              422  AATATT              
TspRI          54  CAGTG                CA[GC]TG[ACGT][ACGT]^
              555  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AfaI        AluI        BssKI       BstNI       DpnII       
EarI        EcoRII      HaeIII      HinfI       Ksp632I     
MaeIII      MboI        MboII       MnlI        MseI        
MvaI        NdeII       NlaIV       RsaI        Sau3AI      
ScrFI       SfaNI       TaqI        TfiI        Tru9I       
Tsp509I     TthHB8I