Restriction map for
pF1KE0414
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AgeI AhdI
AlwNI AocI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BsrI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
Eam1105I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI HphI
KpnI KspI MaeI MamI MfeI
MluI MroI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp509I TspRI Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcyI 100 GGCGCC G[GA]^CG[CT]C
AflIII 167 ACGTGT A^C[GA][CT]GT
Alw44I 346 GTGCAC G^TGCAC
AlwI 84 GATCC GGATC(4/5)
Aor51HI 293 AGCGCT AGC^GCT
ApaLI 346 GTGCAC G^TGCAC
AviII 446 TGCGCA TGC^GCA
BalI 206 TGGCCA TGG^CCA
BanII 520 GAGCTC G[GA]GC[CT]^C
BbeI 100 GGCGCC GGCGC^C
BpmI 319 CTGGAG CTGGAG(16/14)
BsaAI 166 TACGTG [CT]AC^GT[GA]
BsaHI 100 GGCGCC G[GA]^CG[CT]C
BseRI 63 GAGGAG GAGGAG(10/8)
BsgI 424 CTGCAC GTGCAG(16/14)
BsiEI 437 CGGCCG CG[GA][CT]^CG
BsmAI 246 GTCTC GTCTC(1/5)
BsmFI 50 GGGAC GGGAC(10/14)
BspMI 407 ACCTGC ACCTGC(4/8)
BstYI 83 AGATCC [GA]^GATC[CT]
DsaI 260 CCGTGG C^C[GA][CT]GG
EagI 437 CGGCCG C^GGCCG
EarI 547 CTCTTC CTCTTC(1/4)
EclXI 437 CGGCCG C^GGCCG
Eco47III 293 AGCGCT AGC^GCT
Eco52I 437 CGGCCG C^GGCCG
EcoT14I 527 CCAAGG C^C[AT][AT]GG
FauI 97 GCGGG CCCGC(4/6)
FspI 446 TGCGCA TGC^GCA
Hin1I 100 GGCGCC G[GA]^CG[CT]C
KasI 100 GGCGCC G^GCGCC
Ksp632I 547 CTCTTC CTCTTC(1/4)
MaeIII 181 GTGAC ^GT[ACGT]AC
MflI 83 AGATCC [GA]^GATC[CT]
MluNI 206 TGGCCA TGG^CCA
MscI 206 TGGCCA TGG^CCA
MslI 459 CATCGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 100 GGCGCC GG^CGCC
PleI 490 GACTC GAGTC(4/5)
PvuII 142 CAGCTG CAG^CTG
SacI 520 GAGCTC GAGCT^C
SfaNI 155 GCATC GCATC(5/9)
SfcI 333 CTACAG C^T[GA][CT]AG
SfoI 100 GGCGCC GGC^GCC
SmaI 174 CCCGGG CCC^GGG
StyI 527 CCAAGG C^C[AT][AT]GG
TfiI 386 GAATC G^A[AT]TC
Tsp45I 181 GTGAC ^GT[GC]AC
XcmI 239 CCACCAAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 83 AGATCC [GA]^GATC[CT]
XmaCI 174 CCCGGG C^CCGGG
XmaI 174 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AspHI 346 GTGCAC G[AT]GC[AT]^C
520 GAGCTC
AvaI 174 CCCGGG C^[CT]CG[GA]G
217 CCCGAG
BanI 100 GGCGCC G^G[CT][GA]CC
468 GGTGCC
BsiHKAI 346 GTGCAC G[AT]GC[AT]^C
520 GAGCTC
BsiYI 47 CCGGGGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
153 CCGCATCCTGG
BslI 47 CCGGGGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
153 CCGCATCCTGG
BsoBI 174 CCCGGG C^[CT]CG[GA]G
217 CCCGAG
BstF5I 156 CATCC GGATG(2/0)
213 CATCC
BstXI 242 CCAAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
476 CCAAGTTCCTGG
DdeI 11 CTAAG C^T[ACGT]AG
307 CTGAG
EaeI 206 TGGCCA [CT]^GGCC[GA]
437 CGGCCG
FokI 156 CATCC GGATG(9/13)
213 CATCC
HaeII 100 GGCGCC [GA]GCGC^[CT]
293 AGCGCT
HinfI 386 GAATC G^A[ACGT]TC
490 GACTC
MboII 222 GAAGA GAAGA(8/7)
548 TCTTC
MspA1I 142 CAGCTG C[AC]G^C[GT]G
397 CAGCGG
TaqI 227 TCGA T^CGA
461 TCGA
TthHB8I 227 TCGA T^CGA
461 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BcnI BmyI BsaJI Bsp1286I BssHII
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DpnII EcoRII Fnu4HI HaeIII
HapII HgaI HhaI HinP1I HpaII
ItaI MaeII MboI MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIV Sau3AI Sau96I ScrFI TseI