Restriction map for
pF1KB6477
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccIII AclI AfaI
AflII AflIII AgeI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AvaI AviII AvrII
BalI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BstBI BstEII BstPI
BstXI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeIII HapII HgaI HincII HindII
HpaI HpaII HphI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MseI MslI MspI MunI
NaeI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tru9I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 780 GTATAC GT^[AC][GT]AC
AcyI 725 GGCGCC G[GA]^CG[CT]C
AhdI 113 GACCCATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 499 CCTCAGG CC^T[ACGT]AGG
AspEI 113 GACCCATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 139 GAGCTC G[AT]GC[AT]^C
AspI 72 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 504 GGATCC G^GATCC
BbeI 725 GGCGCC GGCGC^C
BglI 277 GCCAGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 725 GGCGCC G[GA]^CG[CT]C
BsgI 67 GTGCAG GTGCAG(16/14)
BsiHKAI 139 GAGCTC G[AT]GC[AT]^C
BsmAI 121 GTCTC GTCTC(1/5)
BsmBI 120 CGTCTC CGTCTC(1/5)
BsrI 741 ACTGG ACTGG(1/-1)
BssSI 851 CACGAG CACGAG(-5/-1)
Bst1107I 780 GTATAC GTA^TAC
BstAPI 60 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 780 GTATAC GTA^TAC
Bsu36I 499 CCTCAGG CC^T[ACGT]AGG
Eam1105I 113 GACCCATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 421 GAAGAG CTCTTC(1/4)
Eco81I 499 CCTCAGG CC^T[ACGT]AGG
EcoNI 508 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 226 GAATTC G^AATTC
FauI 109 GCGGG CCCGC(4/6)
Hin1I 725 GGCGCC G[GA]^CG[CT]C
KasI 725 GGCGCC G^GCGCC
Ksp632I 421 GAAGAG CTCTTC(1/4)
NarI 725 GGCGCC GG^CGCC
NspI 697 GCATGT [GA]CATG^[CT]
PflFI 72 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 512 CTGCAG CTGCA^G
SacI 139 GAGCTC GAGCT^C
SfcI 512 CTGCAG C^T[GA][CT]AG
SfoI 725 GGCGCC GGC^GCC
Tsp45I 925 GTCAC ^GT[GC]AC
TspRI 911 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 72 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 108 CGCG CG^CG
136 CGCG
BanI 131 GGCACC G^G[CT][GA]CC
725 GGCGCC
BanII 139 GAGCTC G[GA]GC[CT]^C
399 GAGCCC
BlpI 45 GCTGAGC GC^T[ACGT]AGC
935 GCTTAGC
BmyI 139 GAGCTC G[AGT]GC[ACT]^C
399 GAGCCC
BpmI 25 CTGGAG CTGGAG(16/14)
742 CTGGAG
Bpu1102I 45 GCTGAGC GC^T[ACGT]AGC
935 GCTTAGC
Bsp1286I 139 GAGCTC G[AGT]GC[ACT]^C
399 GAGCCC
BstUI 108 CGCG CG^CG
136 CGCG
BstYI 504 GGATCC [GA]^GATC[CT]
555 GGATCT
CelII 45 GCTGAGC GC^T[ACGT]AGC
935 GCTTAGC
CfoI 22 GCGC GCG^C
726 GCGC
DpnII 505 GATC ^GATC
556 GATC
HaeII 21 GGCGCT [GA]GCGC^[CT]
725 GGCGCC
HhaI 22 GCGC GCG^C
726 GCGC
HinP1I 22 GCGC G^CGC
726 GCGC
MaeII 672 ACGT A^CGT
923 ACGT
MboI 505 GATC ^GATC
556 GATC
MflI 504 GGATCC [GA]^GATC[CT]
555 GGATCT
MvnI 108 CGCG CG^CG
136 CGCG
NdeII 505 GATC ^GATC
556 GATC
NlaIII 271 CATG CATG^
698 CATG
PleI 391 GACTC GAGTC(4/5)
16 GAGTC
Sau3AI 505 GATC ^GATC
556 GATC
TfiI 244 GAATC G^A[AT]TC
789 GAATC
XhoII 504 GGATCC [GA]^GATC[CT]
555 GGATCT
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AvaII BbvI BfaI BsaJI
BseRI BsiYI BslI BsmFI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII EcoO109I EcoRII Fnu4HI FokI
HindIII HinfI ItaI MaeI MaeIII
MboII MnlI MspA1I MvaI MwoI
NlaIV PpuMI PvuII Sau96I ScrFI
SfaNI TseI Tsp509I