Restriction map for
pF1KE0330
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspI AviII BalI BbrPI BcgI
BciVI BclI BfrI BsaAI BsaBI
BsaI BsaWI BseAI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI EarI Eco47III
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KpnI
Ksp632I MamI MfeI MluI MluNI
MroI MscI MslI MunI NcoI
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AhdI 1287 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 107 GGGCCC GGGCC^C
AspEI 1287 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 579 CCTAGG C^CTAGG
BamHI 25 GGATCC G^GATCC
BbeI 146 GGCGCC GGCGC^C
BbsI 591 GTCTTC GAAGAC(2/6)
BglII 585 AGATCT A^GATCT
BlnI 579 CCTAGG C^CTAGG
BpuAI 591 GTCTTC GAAGAC(2/6)
BsgI 1781 CTGCAC GTGCAG(16/14)
BsiEI 99 CGGCCG CG[GA][CT]^CG
BspHI 1248 TCATGA T^CATGA
BstAPI 252 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1119 CCAAAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 99 CGGCCG C^GGCCG
Eam1105I 1287 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 99 CGGCCG C^GGCCG
Eco52I 99 CGGCCG C^GGCCG
EcoRI 1300 GAATTC G^AATTC
EcoT14I 579 CCTAGG C^C[AT][AT]GG
HaeII 146 GGCGCC [GA]GCGC^[CT]
HgaI 235 GACGC GACGC(5/10)
KasI 146 GGCGCC G^GCGCC
MaeII 919 ACGT A^CGT
NarI 146 GGCGCC GG^CGCC
NdeI 1799 CATATG CA^TATG
PaeR7I 44 CTCGAG C^TCGAG
PflMI 945 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1248 TCATGA T^CATGA
SfoI 146 GGCGCC GGC^GCC
SpeI 55 ACTAGT A^CTAGT
StyI 579 CCTAGG C^C[AT][AT]GG
Van91I 945 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1049 CCACGGCCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 44 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AcsI 574 AAATTC [GA]^AATT[CT]
1300 GAATTC
AcyI 146 GGCGCC G[GA]^CG[CT]C
235 GACGCC
AlwNI 253 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
285 CAGGATCTG
AocI 1227 CCTGAGG CC^T[ACGT]AGG
1749 CCTGAGG
ApoI 574 AAATTC [GA]^AATT[CT]
1300 GAATTC
Asp700I 531 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1372 GAAGGTTTTC
AvaI 44 CTCGAG C^[CT]CG[GA]G
201 CTCGGG
BbvCI 1183 GCTGAGG CCTCAGC(-5/-2)
1687 GCTGAGG
BcnI 140 CCCGG CC^[GC]GG
172 CCGGG
BglI 32 GCCTGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1054 GCCAGCATGGC
BlpI 357 GCTGAGC GC^T[ACGT]AGC
1641 GCTCAGC
BpmI 943 CTCCAG CTGGAG(16/14)
1306 CTGGAG
Bpu1102I 357 GCTGAGC GC^T[ACGT]AGC
1641 GCTCAGC
BsaHI 146 GGCGCC G[GA]^CG[CT]C
235 GACGCC
BsoBI 44 CTCGAG C^[CT]CG[GA]G
201 CTCGGG
BspMI 889 ACCTGC ACCTGC(4/8)
162 GCAGGT
BsrFI 37 GCCGGC [GA]^CCGG[CT]
97 GCCGGC
Bsu36I 1227 CCTGAGG CC^T[ACGT]AGG
1749 CCTGAGG
CelII 357 GCTGAGC GC^T[ACGT]AGC
1641 GCTCAGC
Cfr10I 37 GCCGGC [GA]^CCGG[CT]
97 GCCGGC
DraIII 897 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1132 CACCTAGTG
Eco81I 1227 CCTGAGG CC^T[ACGT]AGG
1749 CCTGAGG
EcoNI 842 CCTCTGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1683 CCTGGCTGAGG
Hin1I 146 GGCGCC G[GA]^CG[CT]C
235 GACGCC
HindIII 605 AAGCTT A^AGCTT
1102 AAGCTT
NaeI 37 GCCGGC GCC^GGC
97 GCCGGC
NciI 140 CCCGG CC^[GC]GG
172 CCGGG
NgoMIV 37 GCCGGC G^CCGGC
97 GCCGGC
PstI 1193 CTGCAG CTGCA^G
1759 CTGCAG
SacI 1309 GAGCTC GAGCT^C
1393 GAGCTC
SfaNI 939 GATGC GCATC(5/9)
1451 GCATC
Tsp45I 453 GTCAC ^GT[GC]AC
739 GTCAC
XmnI 531 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1372 GAAGGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BanI BanII BbvI
BfaI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI BsmI Bsp1286I
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI KspI MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
PpuMI RsaI SacII Sau3AI Sau96I
ScrFI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII