Restriction map for
pF1KB7349
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# NO CUT ENZYMES ( 92 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AspI AvrII BbeI
BbrPI BciVI BclI BfrI BglII
BlnI BsaAI BsaBI BsaWI BseAI
BsiWI Bsp1407I BspEI BspLU11I BsrDI
BsrGI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Eam1105I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 16 GACGTC GACGT^C
AcsI 1951 AAATTC [GA]^AATT[CT]
AflIII 35 ACGTGT A^C[GA][CT]GT
Alw44I 628 GTGCAC G^TGCAC
ApaLI 628 GTGCAC G^TGCAC
ApoI 1951 AAATTC [GA]^AATT[CT]
AviII 110 TGCGCA TGC^GCA
BalI 917 TGGCCA TGG^CCA
BanI 2962 GGTGCC G^G[CT][GA]CC
BbvCI 1768 GCTGAGG CCTCAGC(-5/-2)
BcgI 1197 CGAGTCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 2467 CTAG C^TAG
BsmBI 1135 GAGACG CGTCTC(1/5)
BspDI 1285 ATCGAT AT^CGAT
BspHI 2024 TCATGA T^CATGA
BssHII 569 GCGCGC G^CGCGC
ClaI 1285 ATCGAT AT^CGAT
CpoI 1208 CGGTCCG CG^G[AT]CCG
DraI 2760 TTTAAA TTT^AAA
DraIII 2699 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1007 CGGCCG C^GGCCG
EclXI 1007 CGGCCG C^GGCCG
Eco52I 1007 CGGCCG C^GGCCG
EcoNI 387 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 110 TGCGCA TGC^GCA
HindIII 496 AAGCTT A^AGCTT
MaeI 2467 CTAG C^TAG
MluNI 917 TGGCCA TGG^CCA
MscI 917 TGGCCA TGG^CCA
MseI 2761 TTAA T^TAA
NotI 1006 GCGGCCGC GC^GGCCGC
NspI 1973 ACATGC [GA]CATG^[CT]
PflMI 1854 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 2024 TCATGA T^CATGA
RsrII 1208 CGGTCCG CG^G[AT]CCG
SmaI 2843 CCCGGG CCC^GGG
Tru9I 2761 TTAA T^TAA
Van91I 1854 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1283 CCATCGATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2843 CCCGGG C^CCGGG
XmaI 2843 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 16 GACGTC G[GA]^CG[CT]C
1141 GACGCC
AocI 757 CCTGAGG CC^T[ACGT]AGG
1896 CCTGAGG
ApaI 1392 GGGCCC GGGCC^C
2413 GGGCCC
Asp700I 280 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1458 GAATGCCTTC
BamHI 1640 GGATCC G^GATCC
2901 GGATCC
BglI 1225 GCCAGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1784 GCCTCCGGGGC
BsaHI 16 GACGTC G[GA]^CG[CT]C
1141 GACGCC
BsaI 613 GAGACC GGTCTC(1/5)
198 GGTCTC
BsiEI 346 CGACCG CG[GA][CT]^CG
1007 CGGCCG
BsrFI 104 ACCGGC [GA]^CCGG[CT]
148 ACCGGC
BssSI 937 CACGAG CACGAG(-5/-1)
1195 CACGAG
Bsu36I 757 CCTGAGG CC^T[ACGT]AGG
1896 CCTGAGG
Cfr10I 104 ACCGGC [GA]^CCGG[CT]
148 ACCGGC
DrdI 1191 GACACACGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2641 GACCCCTGGGTC
DsaI 174 CCGTGG C^C[GA][CT]GG
2283 CCGTGG
Eco81I 757 CCTGAGG CC^T[ACGT]AGG
1896 CCTGAGG
HgaI 1141 GACGC GACGC(5/10)
2244 GACGC
Hin1I 16 GACGTC G[GA]^CG[CT]C
1141 GACGCC
SapI 280 GAAGAGC GCTCTTC(1/4)
2019 GCTCTTC
TfiI 1566 GATTC G^A[AT]TC
1726 GATTC
XmnI 280 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1458 GAATGCCTTC
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# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI Aor51HI AspHI AvaI AvaII
BanII BbsI BbvI BcnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII EaeI EarI Eco47III EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PaeR7I PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoI XhoII