Restriction map for
pF1KB7335
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AflIII AgeI AhdI AscI Asp700I
AspEI AvrII BamHI BbeI BbrPI
BbvCI BciVI BfrI BglI BlnI
BsaAI BsaBI BsaWI BseAI BsiEI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV FseI HincII
HindII HindIII HpaI KasI KspI
MamI MluI MroI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SphI Sse8387I SspBI SwaI
XbaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 243 GGTACC G^GTACC
AccI 1018 GTCTAC GT^[AC][GT]AC
AcsI 1710 AAATTC [GA]^AATT[CT]
Alw44I 1424 GTGCAC G^TGCAC
AocI 1351 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2387 AGCGCT AGC^GCT
ApaI 1356 GGGCCC GGGCC^C
ApaLI 1424 GTGCAC G^TGCAC
ApoI 1710 AAATTC [GA]^AATT[CT]
AseI 933 ATTAAT AT^TAAT
AsnI 933 ATTAAT AT^TAAT
Asp718I 243 GGTACC G^GTACC
AspI 1192 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 634 GAAGAC GAAGAC(2/6)
BclI 782 TGATCA T^GATCA
BpuAI 634 GAAGAC GAAGAC(2/6)
BsiWI 1212 CGTACG C^GTACG
BsmBI 681 CGTCTC CGTCTC(1/5)
BstXI 1638 CCAGCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1351 CCTGAGG CC^T[ACGT]AGG
DraIII 493 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2947 GACACCTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2387 AGCGCT AGC^GCT
Eco81I 1351 CCTGAGG CC^T[ACGT]AGG
FbaI 782 TGATCA T^GATCA
KpnI 243 GGTACC GGTAC^C
MaeII 2791 ACGT A^CGT
MfeI 1270 CAATTG C^AATTG
MunI 1270 CAATTG C^AATTG
PaeR7I 1361 CTCGAG C^TCGAG
PflFI 1192 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 482 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 933 ATTAAT AT^TAAT
SacI 2701 GAGCTC GAGCT^C
SpeI 1884 ACTAGT A^CTAGT
SspI 1079 AATATT AAT^ATT
StuI 461 AGGCCT AGG^CCT
Tth111I 1192 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 840 CCAGCTTCGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1361 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 679 GGCGTC G[GA]^CG[CT]C
2358 GGCGTC
AviII 1052 TGCGCA TGC^GCA
1310 TGCGCA
BanII 1356 GGGCCC G[GA]GC[CT]^C
2701 GAGCTC
BcgI 1434 GCAGCCCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1731 GCACATGCATCG
BglII 344 AGATCT A^GATCT
443 AGATCT
BlpI 2339 GCTCAGC GC^T[ACGT]AGC
2850 GCTGAGC
Bpu1102I 2339 GCTCAGC GC^T[ACGT]AGC
2850 GCTGAGC
BsaHI 679 GGCGTC G[GA]^CG[CT]C
2358 GGCGTC
BsaI 1122 GGTCTC GGTCTC(1/5)
2919 GGTCTC
BspMI 2950 ACCTGC ACCTGC(4/8)
42 GCAGGT
BsrBI 2337 CCGCTC CCGCTC(-3/-3)
1581 GAGCGG
BsrFI 2022 ACCGGC [GA]^CCGG[CT]
2204 ACCGGC
BstEII 258 GGTCACC G^GT[ACGT]ACC
795 GGTGACC
BstPI 258 GGTCACC G^GT[ACGT]ACC
795 GGTGACC
CelII 2339 GCTCAGC GC^T[ACGT]AGC
2850 GCTGAGC
Cfr10I 2022 ACCGGC [GA]^CCGG[CT]
2204 ACCGGC
EcoNI 573 CCTCTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3047 CCTGGGGCAGG
EcoO65I 258 GGTCACC G^GT[ACGT]ACC
795 GGTGACC
EcoT22I 1735 ATGCAT ATGCA^T
1980 ATGCAT
FspI 1052 TGCGCA TGC^GCA
1310 TGCGCA
Hin1I 679 GGCGTC G[GA]^CG[CT]C
2358 GGCGTC
NcoI 686 CCATGG C^CATGG
1700 CCATGG
NsiI 1735 ATGCAT ATGCA^T
1980 ATGCAT
NspI 1307 ACATGC [GA]CATG^[CT]
1733 ACATGC
SmaI 254 CCCGGG CCC^GGG
1794 CCCGGG
SmlI 1361 CTCGAG C^T[CT][GA]AG
2049 CTTGAG
XmaCI 254 CCCGGG C^CCGGG
1794 CCCGGG
XmaI 254 CCCGGG C^CCGGG
1794 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BalI BanI
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII