Restriction map for
pF1KE0046
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI Alw44I AocI ApaLI
ApoI AscI AseI AsnI AspI
AviII AvrII BbrPI BcgI BciVI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BsaAI BsaI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrGI Bst1107I BstAPI
BstBI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI MaeI
MaeII MfeI MroI MseI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SalI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tru9I Tth111I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 734 GGTACC G^GTACC
AflIII 1754 ACGCGT A^C[GA][CT]GT
AhdI 1114 GACTATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 642 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1373 AGCGCT AGC^GCT
Asp718I 734 GGTACC G^GTACC
AspEI 1114 GACTATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 577 GTGCTC G[AT]GC[AT]^C
BalI 92 TGGCCA TGG^CCA
BamHI 896 GGATCC G^GATCC
BbeI 1621 GGCGCC GGCGC^C
BbsI 589 GTCTTC GAAGAC(2/6)
BglI 1628 GCCGCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 384 AGATCT A^GATCT
BpuAI 589 GTCTTC GAAGAC(2/6)
BsaBI 379 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 160 GTGCAG GTGCAG(16/14)
BsiHKAI 577 GTGCTC G[AT]GC[AT]^C
BsmBI 1645 GAGACG CGTCTC(1/5)
BspHI 375 TCATGA T^CATGA
BssSI 1345 CACGAG CACGAG(-5/-1)
BstEII 889 GGTTACC G^GT[ACGT]ACC
BstPI 889 GGTTACC G^GT[ACGT]ACC
DraIII 848 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1114 GACTATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1373 AGCGCT AGC^GCT
EcoO65I 889 GGTTACC G^GT[ACGT]ACC
HindIII 400 AAGCTT A^AGCTT
KasI 1621 GGCGCC G^GCGCC
KpnI 734 GGTACC GGTAC^C
MamI 379 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1754 ACGCGT A^CGCGT
MluNI 92 TGGCCA TGG^CCA
MscI 92 TGGCCA TGG^CCA
NarI 1621 GGCGCC GG^CGCC
NotI 1817 GCGGCCGC GC^GGCCGC
PstI 152 CTGCAG CTGCA^G
PvuII 642 CAGCTG CAG^CTG
RcaI 375 TCATGA T^CATGA
SapI 99 GCTCTTC GCTCTTC(1/4)
ScaI 1427 AGTACT AGT^ACT
SfoI 1621 GGCGCC GGC^GCC
SmaI 224 CCCGGG CCC^GGG
SmlI 695 CTCAAG C^T[CT][GA]AG
SphI 812 GCATGC GCATG^C
StuI 57 AGGCCT AGG^CCT
XmaCI 224 CCCGGG C^CCGGG
XmaI 224 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
ApaI 227 GGGCCC GGGCC^C
1159 GGGCCC
Asp700I 55 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1245 GAAGAACTTC
AvaI 224 CCCGGG C^[CT]CG[GA]G
1615 CCCGAG
AvaII 261 GGACC G^G[AT]CC
930 GGTCC
BbvCI 1163 CCTCAGC CCTCAGC(-5/-2)
15 GCTGAGG
BsaWI 751 TCCGGT [AT]^CCGG[AT]
893 ACCGGA
BsiEI 1268 CGGCCG CG[GA][CT]^CG
1818 CGGCCG
BsoBI 224 CCCGGG C^[CT]CG[GA]G
1615 CCCGAG
BspMI 1526 ACCTGC ACCTGC(4/8)
1826 ACCTGC
BsrFI 1266 GCCGGC [GA]^CCGG[CT]
1726 GCCGGC
Cfr10I 1266 GCCGGC [GA]^CCGG[CT]
1726 GCCGGC
EagI 1268 CGGCCG C^GGCCG
1818 CGGCCG
EclXI 1268 CGGCCG C^GGCCG
1818 CGGCCG
Eco52I 1268 CGGCCG C^GGCCG
1818 CGGCCG
EcoNI 787 CCTTTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1023 CCTACATGAGG
MslI 813 CATGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1257 CACGGGGGTG
NaeI 1266 GCCGGC GCC^GGC
1726 GCCGGC
NgoMIV 1266 GCCGGC G^CCGGC
1726 GCCGGC
NspI 212 GCATGT [GA]CATG^[CT]
812 GCATGC
PpuMI 260 GGGACCC [GA]G^G[AT]CC[CT]
929 AGGTCCT
TfiI 202 GAATC G^A[AT]TC
679 GATTC
Tsp509I 393 AATT ^AATT
1042 AATT
XcmI 530 CCAGTTTTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1277 CCATGGACGAGCTGG
XmnI 55 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1245 GAAGAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI BanI BanII BbvI BcnI
BmyI BpmI BsaHI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssHII BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I XhoII