Restriction map for
pF1KB8907
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I CelII CpoI
DraI DraIII EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
Ksp632I MamI MfeI MluI MluNI
MroI MscI MseI MunI NaeI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp509I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 85 CGCG CG^CG
AlwI 225 GATCC GGATC(4/5)
AlwNI 232 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 477 GGGCCC GGGCC^C
AspI 418 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 121 GGCGCC G^G[CT][GA]CC
BbeI 121 GGCGCC GGCGC^C
BbsI 63 GAAGAC GAAGAC(2/6)
BglI 338 GCCTGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 63 GAAGAC GAAGAC(2/6)
BseRI 283 GAGGAG GAGGAG(10/8)
BsiEI 408 CGACCG CG[GA][CT]^CG
BsmAI 424 GTCTC GTCTC(1/5)
BspDI 214 ATCGAT AT^CGAT
BsrDI 307 CATTGC GCAATG(2/0)
BstEII 240 GGTGACC G^GT[ACGT]ACC
BstPI 240 GGTGACC G^GT[ACGT]ACC
BstUI 85 CGCG CG^CG
BstXI 458 CCAGCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 224 AGATCC [GA]^GATC[CT]
ClaI 214 ATCGAT AT^CGAT
DraII 476 AGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 9 GACCGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 153 CGGCCA [CT]^GGCC[GA]
EcoO109I 476 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 240 GGTGACC G^GT[ACGT]ACC
HincII 297 GTTGAC GT[CT]^[GA]AC
HindII 297 GTTGAC GT[CT]^[GA]AC
KasI 121 GGCGCC G^GCGCC
KspI 84 CCGCGG CCGC^GG
MflI 224 AGATCC [GA]^GATC[CT]
MslI 213 CATCGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 85 CGCG CG^CG
NarI 121 GGCGCC GG^CGCC
NlaIII 177 CATG CATG^
PflFI 418 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 156 CCACGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 229 CTGCAG CTGCA^G
SacII 84 CCGCGG CCGC^GG
SfaNI 257 GCATC GCATC(5/9)
SfcI 229 CTGCAG C^T[GA][CT]AG
SfoI 121 GGCGCC GGC^GCC
Tth111I 418 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 156 CCACGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 224 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AvaII 8 GGACC G^G[AT]CC
625 GGACC
BfaI 146 CTAG C^TAG
574 CTAG
BspMI 32 ACCTGC ACCTGC(4/8)
203 ACCTGC
BsrBI 360 CCGCTC CCGCTC(-3/-3)
286 GAGCGG
BsrFI 151 ACCGGC [GA]^CCGG[CT]
410 ACCGGC
Cfr10I 151 ACCGGC [GA]^CCGG[CT]
410 ACCGGC
DdeI 548 CTGAG C^T[ACGT]AG
632 CTGAG
DpnII 174 GATC ^GATC
225 GATC
DsaI 11 CCGTGG C^C[GA][CT]GG
84 CCGCGG
HaeII 121 GGCGCC [GA]GCGC^[CT]
180 GGCGCT
HapII 152 CCGG C^CGG
411 CCGG
HgaI 24 GACGC GACGC(5/10)
142 GCGTC
HinfI 16 GAGTC G^A[ACGT]TC
634 GAGTC
HpaII 152 CCGG C^CGG
411 CCGG
MaeI 146 CTAG C^TAG
574 CTAG
MaeII 295 ACGT A^CGT
495 ACGT
MboI 174 GATC ^GATC
225 GATC
MspI 152 CCGG C^CGG
411 CCGG
NdeII 174 GATC ^GATC
225 GATC
PleI 16 GAGTC GAGTC(4/5)
634 GAGTC
PvuII 235 CAGCTG CAG^CTG
375 CAGCTG
Sau3AI 174 GATC ^GATC
225 GATC
SmlI 363 CTCAAG C^T[CT][GA]AG
582 CTTGAG
XcmI 404 CCACCGACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
542 CCAGGACTGAGGTGG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AcyI AluI BanII BbvI
BmyI BsaHI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I EcoRII
FauI Fnu4HI FokI HaeIII HhaI
Hin1I HinP1I HphI ItaI MaeIII
MboII MnlI MspA1I MvaI MwoI
NlaIV Sau96I ScrFI TaqI TseI
Tsp45I TspRI TthHB8I