Restriction map for
pF1KE0119
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BciVI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstXI Bsu36I CelII Cfr10I
Cfr13I ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MaeII MamI MluI
MluNI MroI MscI MspA1I MspI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 54 CGCG CG^CG
AflIII 474 ACATGT A^C[GA][CT]GT
Alw44I 431 GTGCAC G^TGCAC
ApaLI 431 GTGCAC G^TGCAC
Asp700I 844 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 431 GTGCAC G[AT]GC[AT]^C
BbsI 112 GAAGAC GAAGAC(2/6)
BbvI 57 GCAGC GCAGC(8/12)
BcgI 924 GCAACAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 516 TGATCA T^GATCA
BfaI 353 CTAG C^TAG
BmyI 431 GTGCAC G[AGT]GC[ACT]^C
BpmI 77 CTGGAG CTGGAG(16/14)
BpuAI 112 GAAGAC GAAGAC(2/6)
BsiHKAI 431 GTGCAC G[AT]GC[AT]^C
BsiYI 781 CCCATATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 781 CCCATATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 431 GTGCAC G[AGT]GC[ACT]^C
BspLU11I 474 ACATGT A^CATGT
BsrBI 823 CCGCTC CCGCTC(-3/-3)
Bst1107I 599 GTATAC GTA^TAC
BstUI 54 CGCG CG^CG
BstYI 711 AGATCC [GA]^GATC[CT]
BstZ17I 599 GTATAC GTA^TAC
EcoRI 167 GAATTC G^AATTC
FbaI 516 TGATCA T^GATCA
HaeIII 268 GGCC GG^CC
HphI 992 TCACC GGTGA(8/7)
MaeI 353 CTAG C^TAG
MfeI 219 CAATTG C^AATTG
MflI 711 AGATCC [GA]^GATC[CT]
MunI 219 CAATTG C^AATTG
MvnI 54 CGCG CG^CG
ScaI 307 AGTACT AGT^ACT
SfcI 997 CTACAG C^T[GA][CT]AG
SphI 567 GCATGC GCATG^C
SspI 232 AATATT AAT^ATT
TseI 57 GCAGC G^C[AT]GC
Tsp45I 881 GTCAC ^GT[GC]AC
TspRI 513 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 711 AGATCC [GA]^GATC[CT]
XmnI 844 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 143 GTAGAC GT^[AC][GT]AC
599 GTATAC
AciI 53 CCGC CCGC(-3/-1)
823 CCGC
AfaI 308 GTAC GT^AC
379 GTAC
AlwI 712 GATCC GGATC(4/5)
27 GGATC
BsmFI 1002 GGGAC GGGAC(10/14)
779 GTCCC
BssKI 76 CCTGG ^CC[ACGT]GG
343 CCTGG
BstNI 76 CCTGG CC^[AT]GG
343 CCTGG
Cac8I 567 GCATGC GC[ACGT]^[ACGT]GC
963 GCTTGC
CfoI 16 GCGC GCG^C
55 GCGC
DdeI 454 CTAAG C^T[ACGT]AG
674 CTCAG
DraI 418 TTTAAA TTT^AAA
703 TTTAAA
EcoRII 76 CCTGG ^CC[AT]GG
343 CCTGG
Fnu4HI 57 GCAGC GC^[ACGT]GC
822 GCCGC
HhaI 16 GCGC GCG^C
55 GCGC
HinP1I 16 GCGC G^CGC
55 GCGC
ItaI 57 GCAGC GC^[ACGT]GC
822 GCCGC
MslI 475 CATGTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
883 CACACGGATG
MvaI 76 CCTGG CC^[AT]GG
343 CCTGG
NlaIII 475 CATG CATG^
568 CATG
NlaIV 339 GGTTCC GG[ACGT]^[ACGT]CC
532 GGAACC
NspI 474 ACATGT [GA]CATG^[CT]
567 GCATGC
RsaI 308 GTAC GT^AC
379 GTAC
ScrFI 76 CCTGG CC^[ACGT]GG
343 CCTGG
SfaNI 13 GATGC GCATC(5/9)
889 GATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AcsI AluI ApoI BsrI BstF5I
DpnII FokI HinfI MaeIII MboI
MboII MnlI MseI MwoI NdeII
PleI Sau3AI TaqI TfiI Tru9I
Tsp509I TthHB8I