Restriction map for
pF1KE0558
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI AocI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BalI BamHI BbrPI BbvCI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BsrI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HgaI
HindIII HpaI KpnI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RcaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcsI 754 GAATTT [GA]^AATT[CT]
AfaI 864 GTAC GT^AC
AlwI 657 GATCC GGATC(4/5)
ApoI 754 GAATTT [GA]^AATT[CT]
AspI 976 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 852 CCCGGG C^[CT]CG[GA]G
AvaII 306 GGTCC G^G[AT]CC
AviII 512 TGCGCA TGC^GCA
BcgI 807 GCAGCACGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 751 CTAG C^TAG
BglI 593 GCCTCTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 266 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 266 GCTCAGC GC^T[ACGT]AGC
BsaWI 26 ACCGGA [AT]^CCGG[AT]
BseRI 550 GAGGAG GAGGAG(10/8)
BsmAI 83 GTCTC GTCTC(1/5)
BsoBI 852 CCCGGG C^[CT]CG[GA]G
BsrBI 478 GAGCGG CCGCTC(-3/-3)
BstAPI 1119 GCACAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 656 AGATCC [GA]^GATC[CT]
CelII 266 GCTCAGC GC^T[ACGT]AGC
EcoT14I 218 CCATGG C^C[AT][AT]GG
FspI 512 TGCGCA TGC^GCA
HincII 733 GTCAAC GT[CT]^[GA]AC
HindII 733 GTCAAC GT[CT]^[GA]AC
HinfI 30 GAGTC G^A[ACGT]TC
KspI 488 CCGCGG CCGC^GG
MaeI 751 CTAG C^TAG
MflI 656 AGATCC [GA]^GATC[CT]
NaeI 916 GCCGGC GCC^GGC
NcoI 218 CCATGG C^CATGG
NgoMIV 916 GCCGGC G^CCGGC
NspI 992 GCATGT [GA]CATG^[CT]
PflFI 976 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 303 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 30 GAGTC GAGTC(4/5)
PpuMI 305 AGGTCCT [GA]G^G[AT]CC[CT]
PvuI 971 CGATCG CGAT^CG
RsaI 864 GTAC GT^AC
SacI 961 GAGCTC GAGCT^C
SacII 488 CCGCGG CCGC^GG
SfaNI 1052 GCATC GCATC(5/9)
SmaI 852 CCCGGG CCC^GGG
StuI 239 AGGCCT AGG^CCT
StyI 218 CCATGG C^C[AT][AT]GG
Tsp509I 755 AATT ^AATT
Tth111I 976 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 303 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 656 AGATCC [GA]^GATC[CT]
XmaCI 852 CCCGGG C^CCGGG
XmaI 852 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AatII 724 GACGTC GACGT^C
979 GACGTC
AccI 22 GTCTAC GT^[AC][GT]AC
400 GTCTAC
Alw44I 1117 GTGCAC G^TGCAC
1162 GTGCAC
ApaI 232 GGGCCC GGGCC^C
857 GGGCCC
ApaLI 1117 GTGCAC G^TGCAC
1162 GTGCAC
BanI 505 GGCGCC G^G[CT][GA]CC
932 GGCGCC
BbeI 505 GGCGCC GGCGC^C
932 GGCGCC
BbsI 586 GAAGAC GAAGAC(2/6)
727 GTCTTC
BpuAI 586 GAAGAC GAAGAC(2/6)
727 GTCTTC
BsiEI 441 CGACCG CG[GA][CT]^CG
971 CGATCG
BsmFI 245 GGGAC GGGAC(10/14)
697 GTCCC
BsrFI 767 ACCGGC [GA]^CCGG[CT]
916 GCCGGC
BstXI 212 CCACGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1106 CCATGCTCGTGG
Cfr10I 767 ACCGGC [GA]^CCGG[CT]
916 GCCGGC
DpnII 657 GATC ^GATC
972 GATC
DrdI 718 GACGATGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
724 GACGTCTTCGTC
EaeI 59 TGGCCG [CT]^GGCC[GA]
914 TGGCCG
EarI 334 CTCTTC CTCTTC(1/4)
520 CTCTTC
FauI 1184 CCCGC CCCGC(4/6)
490 GCGGG
HaeII 505 GGCGCC [GA]GCGC^[CT]
932 GGCGCC
KasI 505 GGCGCC G^GCGCC
932 GGCGCC
Ksp632I 334 CTCTTC CTCTTC(1/4)
520 CTCTTC
MaeIII 64 GTGAC ^GT[ACGT]AC
250 GTGAC
MboI 657 GATC ^GATC
972 GATC
MslI 213 CACGCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1107 CATGCTCGTG
NarI 505 GGCGCC GG^CGCC
932 GGCGCC
NdeII 657 GATC ^GATC
972 GATC
PvuII 160 CAGCTG CAG^CTG
571 CAGCTG
Sau3AI 657 GATC ^GATC
972 GATC
SfoI 505 GGCGCC GGC^GCC
932 GGCGCC
SmlI 649 CTCAAG C^T[CT][GA]AG
667 CTCAAG
TaqI 626 TCGA T^CGA
974 TCGA
Tsp45I 64 GTGAC ^GT[GC]AC
250 GTGAC
TspRI 346 CACTG CA[GC]TG[ACGT][ACGT]^
613 CAGTG
TthHB8I 626 TCGA T^CGA
974 TCGA
XcmI 144 CCAGAAGCCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1148 CCATGTGGGGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AspHI BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BsgI BsiHKAI
BsiYI BslI Bsp1286I BspMI BssKI
BssSI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HpaII
HphI ItaI MaeII MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PstI Sau96I
ScrFI SfcI TseI