Restriction map for
pF1KE0454
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvrII
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BspDI BspEI
BsrBI BsrDI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI Ksp632I MamI
MfeI MluI MroI MslI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I Van91I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
AccII 33 CGCG CG^CG
AflII 645 CTTAAG C^TTAAG
AflIII 611 ACATGT A^C[GA][CT]GT
AgeI 122 ACCGGT A^CCGGT
ApaI 27 GGGCCC GGGCC^C
Asp700I 856 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 409 CTCGAG C^[CT]CG[GA]G
AviII 12 TGCGCA TGC^GCA
BalI 315 TGGCCA TGG^CCA
BamHI 147 GGATCC G^GATCC
BanII 27 GGGCCC G[GA]GC[CT]^C
BcnI 192 CCGGG CC^[GC]GG
BfrI 645 CTTAAG C^TTAAG
BglI 29 GCCCCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 27 GGGCCC G[AGT]GC[ACT]^C
BpmI 301 CTGGAG CTGGAG(16/14)
BsaWI 122 ACCGGT [AT]^CCGG[AT]
BsmI 868 GAATGC GAATGC(1/-1)
BsoBI 409 CTCGAG C^[CT]CG[GA]G
Bsp1286I 27 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 368 TGTACA T^GTACA
BspHI 545 TCATGA T^CATGA
BspLU11I 611 ACATGT A^CATGT
BspMI 377 ACCTGC ACCTGC(4/8)
BsrFI 122 ACCGGT [GA]^CCGG[CT]
BsrGI 368 TGTACA T^GTACA
BsrI 296 ACTGG ACTGG(1/-1)
BstUI 33 CGCG CG^CG
Cac8I 34 GCGGGC GC[ACGT]^[ACGT]GC
CfoI 13 GCGC GCG^C
Cfr10I 122 ACCGGT [GA]^CCGG[CT]
DraI 808 TTTAAA TTT^AAA
DraII 27 GGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 315 TGGCCA [CT]^GGCC[GA]
EcoO109I 27 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 95 CCATGG C^C[AT][AT]GG
FspI 12 TGCGCA TGC^GCA
HhaI 13 GCGC GCG^C
HinP1I 13 GCGC G^CGC
HincII 538 GTCAAC GT[CT]^[GA]AC
HindII 538 GTCAAC GT[CT]^[GA]AC
KspI 32 CCGCGG CCGC^GG
MaeII 825 ACGT A^CGT
MaeIII 641 GTGAC ^GT[ACGT]AC
MluNI 315 TGGCCA TGG^CCA
MscI 315 TGGCCA TGG^CCA
MvnI 33 CGCG CG^CG
NciI 192 CCGGG CC^[GC]GG
NcoI 95 CCATGG C^CATGG
NspI 611 ACATGT [GA]CATG^[CT]
PaeR7I 409 CTCGAG C^TCGAG
PinAI 122 ACCGGT A^CCGGT
RcaI 545 TCATGA T^CATGA
SacII 32 CCGCGG CCGC^GG
ScaI 275 AGTACT AGT^ACT
SfaNI 491 GCATC GCATC(5/9)
SfcI 702 CTACAG C^T[GA][CT]AG
SspBI 368 TGTACA T^GTACA
SspI 857 AATATT AAT^ATT
StyI 95 CCATGG C^C[AT][AT]GG
Tsp45I 641 GTGAC ^GT[GC]AC
XbaI 522 TCTAGA T^CTAGA
XcmI 248 CCAACGGAGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 409 CTCGAG C^TCGAG
XmnI 856 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 276 GTAC GT^AC
369 GTAC
BbsI 796 GAAGAC GAAGAC(2/6)
837 GTCTTC
BfaI 523 CTAG C^TAG
802 CTAG
BpuAI 796 GAAGAC GAAGAC(2/6)
837 GTCTTC
BsmFI 62 GGGAC GGGAC(10/14)
245 GTCCC
DsaI 32 CCGCGG C^C[GA][CT]GG
95 CCATGG
HaeIII 28 GGCC GG^CC
316 GGCC
HapII 123 CCGG C^CGG
192 CCGG
HpaII 123 CCGG C^CGG
192 CCGG
HphI 48 GGTGA GGTGA(8/7)
375 TCACC
MaeI 523 CTAG C^TAG
802 CTAG
MseI 646 TTAA T^TAA
809 TTAA
MspI 123 CCGG C^CGG
192 CCGG
PvuII 16 CAGCTG CAG^CTG
394 CAGCTG
RsaI 276 GTAC GT^AC
369 GTAC
SmlI 409 CTCGAG C^T[CT][GA]AG
645 CTTAAG
Tru9I 646 TTAA T^TAA
809 TTAA
TspRI 106 CACTG CA[GC]TG[ACGT][ACGT]^
415 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BbvI BsaJI BsiYI BslI
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII EcoRII FauI Fnu4HI
FokI HinfI ItaI MboI MboII
MflI MnlI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI TaqI TfiI TseI
Tsp509I TthHB8I XhoII