Restriction map for
pF1KB4953
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# NO CUT ENZYMES ( 104 enzymes) #
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Acc65I AclI AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AvaI AviII AvrII
BbeI BbvCI BciVI BclI BcnI
BglI BlnI BsaBI BsiEI BsiWI
BsoBI BspDI BspHI BsrFI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoT22I FbaI FseI
FspI HgaI HindIII HpaI KasI
KpnI KspI MamI MluI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SwaI Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AatII 1707 GACGTC GACGT^C
AccI 802 GTCTAC GT^[AC][GT]AC
AccIII 2156 TCCGGA T^CCGGA
AcyI 1707 GACGTC G[GA]^CG[CT]C
AflII 853 CTTAAG C^TTAAG
AflIII 2711 ACATGT A^C[GA][CT]GT
Alw44I 1269 GTGCAC G^TGCAC
ApaLI 1269 GTGCAC G^TGCAC
AspI 18 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 17 GGACC G^G[AT]CC
BalI 1200 TGGCCA TGG^CCA
BamHI 2000 GGATCC G^GATCC
BbrPI 3307 CACGTG CAC^GTG
BcgI 1657 CGACTCTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 853 CTTAAG C^TTAAG
BglII 2573 AGATCT A^GATCT
BlpI 944 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 944 GCTCAGC GC^T[ACGT]AGC
BsaAI 3307 CACGTG [CT]AC^GT[GA]
BsaHI 1707 GACGTC G[GA]^CG[CT]C
BsaI 2635 GGTCTC GGTCTC(1/5)
BsaWI 2156 TCCGGA [AT]^CCGG[AT]
BseAI 2156 TCCGGA T^CCGGA
BsmBI 1705 GAGACG CGTCTC(1/5)
BsmFI 2012 GGGAC GGGAC(10/14)
BspEI 2156 TCCGGA T^CCGGA
BspLU11I 2711 ACATGT A^CATGT
BspMI 495 ACCTGC ACCTGC(4/8)
BssHII 39 GCGCGC G^CGCGC
BstXI 3047 CCATTCCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 944 GCTCAGC GC^T[ACGT]AGC
DsaI 3351 CCATGG C^C[GA][CT]GG
EaeI 1200 TGGCCA [CT]^GGCC[GA]
EcoRI 834 GAATTC G^AATTC
EcoRV 613 GATATC GAT^ATC
FauI 1279 GCGGG CCCGC(4/6)
HaeII 1006 GGCGCT [GA]GCGC^[CT]
HapII 2157 CCGG C^CGG
Hin1I 1707 GACGTC G[GA]^CG[CT]C
HincII 625 GTTGAC GT[CT]^[GA]AC
HindII 625 GTTGAC GT[CT]^[GA]AC
HpaII 2157 CCGG C^CGG
MfeI 1504 CAATTG C^AATTG
MluNI 1200 TGGCCA TGG^CCA
MroI 2156 TCCGGA T^CCGGA
MscI 1200 TGGCCA TGG^CCA
MspA1I 133 CAGCTG C[AC]G^C[GT]G
MspI 2157 CCGG C^CGG
MunI 1504 CAATTG C^AATTG
NcoI 3351 CCATGG C^CATGG
NdeI 3413 CATATG CA^TATG
PflFI 18 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3307 CACGTG CAC^GTG
PmlI 3307 CACGTG CAC^GTG
PvuII 133 CAGCTG CAG^CTG
SacI 761 GAGCTC GAGCT^C
SapI 221 GAAGAGC GCTCTTC(1/4)
SbfI 2424 CCTGCAGG CCTGCA^GG
Sse8387I 2424 CCTGCAGG CCTGCA^GG
StuI 2421 AGGCCT AGG^CCT
Tth111I 18 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccII 38 CGCG CG^CG
40 CGCG
AlwNI 1154 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
3208 CAGGAGCTG
Asp700I 962 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3383 GAATCGTTTC
BanI 721 GGCACC G^G[CT][GA]CC
2497 GGTGCC
BbsI 57 GAAGAC GAAGAC(2/6)
78 GAAGAC
BfaI 1010 CTAG C^TAG
1610 CTAG
BpuAI 57 GAAGAC GAAGAC(2/6)
78 GAAGAC
BseRI 2285 CTCCTC GAGGAG(10/8)
758 GAGGAG
BsgI 1373 CTGCAC GTGCAG(16/14)
1852 GTGCAG
BsmI 2748 GAATGC GAATGC(1/-1)
966 GCATTC
BsrBI 33 CCGCTC CCGCTC(-3/-3)
3321 CCGCTC
BstUI 38 CGCG CG^CG
40 CGCG
DraI 2790 TTTAAA TTT^AAA
3184 TTTAAA
MaeI 1010 CTAG C^TAG
1610 CTAG
MvnI 38 CGCG CG^CG
40 CGCG
PstI 259 CTGCAG CTGCA^G
2425 CTGCAG
SspI 2468 AATATT AAT^ATT
2682 AATATT
XmnI 962 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3383 GAATCGTTTC
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BanII BbvI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII