Restriction map for
pF1KE0109
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaII
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRV EcoT22I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaI HphI KasI
KpnI KspI MaeI MaeII MaeIII
MamI MfeI MflI MluI MroI
MseI MslI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
Sau96I SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI TfiI Tru9I Tsp45I
Tth111I TthHB8I Van91I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcsI 102 GAATTC [GA]^AATT[CT]
AfaI 50 GTAC GT^AC
AlwI 39 GATCC GGATC(4/5)
AlwNI 245 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 102 GAATTC [GA]^AATT[CT]
AvaI 179 CCCGGG C^[CT]CG[GA]G
BalI 146 TGGCCA TGG^CCA
BclI 278 TGATCA T^GATCA
BsmBI 302 CGTCTC CGTCTC(1/5)
BsmFI 182 GGGAC GGGAC(10/14)
BsoBI 179 CCCGGG C^[CT]CG[GA]G
BstF5I 393 CATCC GGATG(2/0)
Cac8I 250 GCTGGC GC[ACGT]^[ACGT]GC
DdeI 353 CTGAG C^T[ACGT]AG
DsaI 359 CCATGG C^C[GA][CT]GG
EaeI 146 TGGCCA [CT]^GGCC[GA]
EcoNI 348 CCTGTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 102 GAATTC G^AATTC
EcoT14I 359 CCATGG C^C[AT][AT]GG
FbaI 278 TGATCA T^GATCA
FokI 393 CATCC GGATG(9/13)
MluNI 146 TGGCCA TGG^CCA
MscI 146 TGGCCA TGG^CCA
MspA1I 42 CCGCTG C[AC]G^C[GT]G
MwoI 214 GCTGGGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 359 CCATGG C^CATGG
RsaI 50 GTAC GT^AC
SapI 318 GCTCTTC GCTCTTC(1/4)
SfcI 123 CTACAG C^T[GA][CT]AG
SmaI 179 CCCGGG CCC^GGG
SmlI 307 CTCAAG C^T[CT][GA]AG
StuI 67 AGGCCT AGG^CCT
StyI 359 CCATGG C^C[AT][AT]GG
Tsp509I 103 AATT ^AATT
XcmI 224 CCACAGTTGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 179 CCCGGG C^CCGGG
XmaI 179 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AciI 8 CCGC CCGC(-3/-1)
42 CCGC
BpmI 29 CTCCAG CTGGAG(16/14)
139 CTCCAG
BsiYI 135 CCCACTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
348 CCTGTCTGAGG
BslI 135 CCCACTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
348 CCTGTCTGAGG
BsmAI 175 GTCTC GTCTC(1/5)
303 GTCTC
BsrI 97 CCAGT ACTGG(1/-1)
167 CCAGT
DpnII 39 GATC ^GATC
279 GATC
EarI 319 CTCTTC CTCTTC(1/4)
397 CTCTTC
HapII 11 CCGG C^CGG
180 CCGG
HpaII 11 CCGG C^CGG
180 CCGG
Ksp632I 319 CTCTTC CTCTTC(1/4)
397 CTCTTC
MboI 39 GATC ^GATC
279 GATC
MboII 320 TCTTC GAAGA(8/7)
398 TCTTC
MspI 11 CCGG C^CGG
180 CCGG
NdeII 39 GATC ^GATC
279 GATC
NlaIII 360 CATG CATG^
405 CATG
NlaIV 259 GGAACC GG[ACGT]^[ACGT]CC
296 GGAACC
Sau3AI 39 GATC ^GATC
279 GATC
TspRI 98 CAGTG CA[GC]TG[ACGT][ACGT]^
168 CAGTG
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
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AluI BbvI BcnI BsaJI BssKI
BstNI EcoRII Fnu4HI HaeIII ItaI
MnlI MvaI NciI ScrFI TseI