Restriction map for
pF1KB9540
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI AspHI
AspI AvaI AviII BamHI BbeI
BbrPI BciVI BclI BfrI BglI
BglII BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KspI MaeIII MamI MluI
MroI MspA1I MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Acc65I 881 GGTACC G^GTACC
AhdI 43 GACTTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 994 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 273 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 881 GGTACC G^GTACC
AspEI 43 GACTTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 90 CCTAGG C^CTAGG
BanI 881 GGTACC G^G[CT][GA]CC
BbsI 631 GAAGAC GAAGAC(2/6)
BbvCI 694 CCTCAGC CCTCAGC(-5/-2)
BcnI 239 CCGGG CC^[GC]GG
BlnI 90 CCTAGG C^CTAGG
BpuAI 631 GAAGAC GAAGAC(2/6)
BstYI 989 AGATCC [GA]^GATC[CT]
Eam1105I 43 GACTTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 559 GATATC GAT^ATC
FauI 166 GCGGG CCCGC(4/6)
HapII 239 CCGG C^CGG
HpaII 239 CCGG C^CGG
KpnI 881 GGTACC GGTAC^C
MfeI 419 CAATTG C^AATTG
MflI 989 AGATCC [GA]^GATC[CT]
MseI 442 TTAA T^TAA
MslI 660 CATCTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 239 CCGG C^CGG
MunI 419 CAATTG C^AATTG
NciI 239 CCGGG CC^[GC]GG
PleI 676 GAGTC GAGTC(4/5)
SexAI 455 ACCAGGT A^CC[AT]GGT
TfiI 973 GATTC G^A[AT]TC
Tru9I 442 TTAA T^TAA
Tsp509I 420 AATT ^AATT
XhoII 989 AGATCC [GA]^GATC[CT]
XmnI 273 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 882 GTAC GT^AC
996 GTAC
BcgI 282 CGACTTGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
453 CGACCAGGTTGC
BsaI 94 GGTCTC GGTCTC(1/5)
689 GGTCTC
BseRI 171 CTCCTC GAGGAG(10/8)
692 CTCCTC
Cac8I 166 GCGGGC GC[ACGT]^[ACGT]GC
183 GCTGGC
DraII 503 GGGTCCT [GA]G^G[ACGT]CC[CT]
1046 GGGTCCC
EcoO109I 503 GGGTCCT [GA]G^G[ACGT]CC[CT]
1046 GGGTCCC
EcoT14I 90 CCTAGG C^C[AT][AT]GG
983 CCAAGG
HinfI 676 GAGTC G^A[ACGT]TC
973 GATTC
MaeII 343 ACGT A^CGT
599 ACGT
MwoI 14 GCTTCCTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
595 GCTTACGTTGC
PpuMI 503 GGGTCCT [GA]G^G[AT]CC[CT]
1046 GGGTCCC
RsaI 882 GTAC GT^AC
996 GTAC
StyI 90 CCTAGG C^C[AT][AT]GG
983 CCAAGG
TaqI 59 TCGA T^CGA
281 TCGA
TthHB8I 59 TCGA T^CGA
281 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwI AvaII BalI
BanII BbvI BfaI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII EaeI EarI
EcoNI EcoRII Fnu4HI FokI HaeIII
HphI ItaI Ksp632I MaeI MboI
MboII MluNI MnlI MscI MvaI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI TseI TspRI