Restriction map for
pF1KE0488
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AgeI Alw44I AlwI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspI AviII AvrII BalI
BamHI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BsaBI BsaWI BseAI BseRI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I ClaI CpoI
DpnII DraI DraIII DrdI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HpaI KspI MaeI MaeIII
MamI MboI MfeI MflI MluNI
MroI MscI MseI MunI NcoI
NdeI NdeII NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau3AI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SspBI SspI StuI SwaI
TfiI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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Acc65I 461 GGTACC G^GTACC
AcyI 9 GGCGCC G[GA]^CG[CT]C
AhdI 30 GACTGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 123 CCTGAGG CC^T[ACGT]AGG
Aor51HI 404 AGCGCT AGC^GCT
Asp718I 461 GGTACC G^GTACC
AspEI 30 GACTGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 844 GAGCAC G[AT]GC[AT]^C
BbeI 9 GGCGCC GGCGC^C
BbrPI 229 CACGTG CAC^GTG
BbsI 499 GTCTTC GAAGAC(2/6)
BlpI 819 GCTGAGC GC^T[ACGT]AGC
BpmI 102 CTGGAG CTGGAG(16/14)
Bpu1102I 819 GCTGAGC GC^T[ACGT]AGC
BpuAI 499 GTCTTC GAAGAC(2/6)
BsaHI 9 GGCGCC G[GA]^CG[CT]C
BsaI 607 GAGACC GGTCTC(1/5)
BsgI 345 CTGCAC GTGCAG(16/14)
BsiEI 1064 CGGCCG CG[GA][CT]^CG
BsiHKAI 844 GAGCAC G[AT]GC[AT]^C
BsmBI 258 CGTCTC CGTCTC(1/5)
BstAPI 869 GCATCCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 123 CCTGAGG CC^T[ACGT]AGG
CelII 819 GCTGAGC GC^T[ACGT]AGC
EaeI 1064 CGGCCG [CT]^GGCC[GA]
EagI 1064 CGGCCG C^GGCCG
Eam1105I 30 GACTGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 208 CTCTTC CTCTTC(1/4)
EclXI 1064 CGGCCG C^GGCCG
Eco47III 404 AGCGCT AGC^GCT
Eco52I 1064 CGGCCG C^GGCCG
Eco81I 123 CCTGAGG CC^T[ACGT]AGG
EcoNI 273 CCTCATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 14 CCTTGG C^C[AT][AT]GG
HgaI 544 GACGC GACGC(5/10)
Hin1I 9 GGCGCC G[GA]^CG[CT]C
HincII 265 GTCAAC GT[CT]^[GA]AC
HindII 265 GTCAAC GT[CT]^[GA]AC
HindIII 620 AAGCTT A^AGCTT
HphI 686 TCACC GGTGA(8/7)
KasI 9 GGCGCC G^GCGCC
KpnI 461 GGTACC GGTAC^C
Ksp632I 208 CTCTTC CTCTTC(1/4)
MluI 803 ACGCGT A^CGCGT
NaeI 473 GCCGGC GCC^GGC
NarI 9 GGCGCC GG^CGCC
NgoMIV 473 GCCGGC G^CCGGC
NspI 981 GCATGC [GA]CATG^[CT]
PaeR7I 85 CTCGAG C^TCGAG
PmaCI 229 CACGTG CAC^GTG
PmlI 229 CACGTG CAC^GTG
PpuMI 18 GGGACCC [GA]G^G[AT]CC[CT]
SbfI 387 CCTGCAGG CCTGCA^GG
SfoI 9 GGCGCC GGC^GCC
SmaI 539 CCCGGG CCC^GGG
SmlI 85 CTCGAG C^T[CT][GA]AG
SphI 981 GCATGC GCATG^C
Sse8387I 387 CCTGCAGG CCTGCA^GG
StyI 14 CCTTGG C^C[AT][AT]GG
TaqI 86 TCGA T^CGA
TthHB8I 86 TCGA T^CGA
XhoI 85 CTCGAG C^TCGAG
XmaCI 539 CCCGGG C^CCGGG
XmaI 539 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AccII 448 CGCG CG^CG
804 CGCG
AflIII 314 ACGTGT A^C[GA][CT]GT
803 ACGCGT
AlwNI 370 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
723 CAGGAACTG
BanI 9 GGCGCC G^G[CT][GA]CC
461 GGTACC
BanII 822 GAGCCC G[GA]GC[CT]^C
928 GAGCCC
BsaAI 229 CACGTG [CT]AC^GT[GA]
313 TACGTG
BsmAI 607 GAGAC GTCTC(1/5)
259 GTCTC
BspMI 386 ACCTGC ACCTGC(4/8)
515 ACCTGC
BstUI 448 CGCG CG^CG
804 CGCG
DraII 18 GGGACCC [GA]G^G[ACGT]CC[CT]
359 AGGGCCT
DsaI 742 CCACGG C^C[GA][CT]GG
1018 CCACGG
EcoO109I 18 GGGACCC [GA]G^G[ACGT]CC[CT]
359 AGGGCCT
HinfI 67 GACTC G^A[ACGT]TC
970 GACTC
MslI 183 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
810 CATCTCTGTG
MvnI 448 CGCG CG^CG
804 CGCG
PleI 67 GACTC GAGTC(4/5)
970 GACTC
TspRI 186 CACTG CA[GC]TG[ACGT][ACGT]^
934 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AvaI AvaII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr10I Cfr13I DdeI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeII MboII MnlI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
PstI RsaI Sau96I ScrFI SfaNI
SfcI TseI