Restriction map for
pF1KB6382
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BbvI BcgI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstNI BstPI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DsaI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRII EcoRV
EcoT22I FbaI FseI FspI HaeII
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MflI MluI MluNI MroI MscI
MunI MvaI NaeI NarI NcoI
NdeI NgoMIV NheI NruI NsiI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TseI Van91I XbaI XcmI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AcsI 184 AAATTC [GA]^AATT[CT]
AcyI 637 GACGCC G[GA]^CG[CT]C
AfaI 237 GTAC GT^AC
ApoI 184 AAATTC [GA]^AATT[CT]
Asp700I 298 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 475 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 707 CTCGAG C^[CT]CG[GA]G
BciVI 268 GGATAC GTATCC(6/5)
BcnI 741 CCGGG CC^[GC]GG
BsaAI 791 CACGTA [CT]AC^GT[GA]
BsaHI 637 GACGCC G[GA]^CG[CT]C
BsaI 448 GAGACC GGTCTC(1/5)
BsaJI 314 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 81 ACCGGA [AT]^CCGG[AT]
BsgI 751 CTGCAC GTGCAG(16/14)
BsiEI 65 CGGCCG CG[GA][CT]^CG
BsmBI 37 GAGACG CGTCTC(1/5)
BsmFI 743 GGGAC GGGAC(10/14)
BsoBI 707 CTCGAG C^[CT]CG[GA]G
BspMI 749 ACCTGC ACCTGC(4/8)
BsrI 701 CCAGT ACTGG(1/-1)
BssKI 741 CCGGG ^CC[ACGT]GG
BstAPI 205 GCACATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DdeI 773 CTAAG C^T[ACGT]AG
DraIII 206 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 517 GACGATGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 65 CGGCCG [CT]^GGCC[GA]
EagI 65 CGGCCG C^GGCCG
EarI 397 GAAGAG CTCTTC(1/4)
EclXI 65 CGGCCG C^GGCCG
Eco52I 65 CGGCCG C^GGCCG
EcoNI 6 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 314 CCAAGG C^C[AT][AT]GG
Hin1I 637 GACGCC G[GA]^CG[CT]C
Ksp632I 397 GAAGAG CTCTTC(1/4)
MspA1I 641 CCGCTG C[AC]G^C[GT]G
NciI 741 CCGGG CC^[GC]GG
NotI 64 GCGGCCGC GC^GGCCGC
NspI 341 GCATGT [GA]CATG^[CT]
PaeR7I 707 CTCGAG C^TCGAG
PflFI 475 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 237 GTAC GT^AC
ScrFI 741 CCGGG CC^[ACGT]GG
StuI 776 AGGCCT AGG^CCT
StyI 314 CCAAGG C^C[AT][AT]GG
Tth111I 475 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 707 CTCGAG C^TCGAG
XmnI 298 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 734 ACACGT A^C[GA][CT]GT
790 ACACGT
AluI 336 AGCT AG^CT
623 AGCT
AlwI 3 GATCC GGATC(4/5)
729 GATCC
AspHI 704 GTGCTC G[AT]GC[AT]^C
768 GTGCTC
AvaII 120 GGTCC G^G[AT]CC
591 GGTCC
BbsI 882 GAAGAC GAAGAC(2/6)
490 GTCTTC
BmyI 704 GTGCTC G[AGT]GC[ACT]^C
768 GTGCTC
BpmI 10 CTGGAG CTGGAG(16/14)
619 CTGGAG
BpuAI 882 GAAGAC GAAGAC(2/6)
490 GTCTTC
BseRI 614 CTCCTC GAGGAG(10/8)
34 GAGGAG
BsiHKAI 704 GTGCTC G[AT]GC[AT]^C
768 GTGCTC
Bsp1286I 704 GTGCTC G[AGT]GC[ACT]^C
768 GTGCTC
CfoI 46 GCGC GCG^C
836 GCGC
Cfr13I 120 GGTCC G^G[ACGT]CC
591 GGTCC
DpnII 3 GATC ^GATC
729 GATC
DraII 119 GGGTCCT [GA]G^G[ACGT]CC[CT]
590 AGGTCCT
EcoO109I 119 GGGTCCT [GA]G^G[ACGT]CC[CT]
590 AGGTCCT
FauI 896 CCCGC CCCGC(4/6)
798 GCGGG
HaeIII 66 GGCC GG^CC
777 GGCC
HhaI 46 GCGC GCG^C
836 GCGC
HinP1I 46 GCGC G^CGC
836 GCGC
HphI 15 GGTGA GGTGA(8/7)
649 GGTGA
MboI 3 GATC ^GATC
729 GATC
MseI 226 TTAA T^TAA
506 TTAA
MslI 691 CACACCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
789 CACACGTATG
MwoI 205 GCACATGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
566 GCAGAGCCAGC
NdeII 3 GATC ^GATC
729 GATC
NlaIV 119 GGGTCC GG[ACGT]^[ACGT]CC
609 GGAACC
PleI 463 GAGTC GAGTC(4/5)
479 GAGTC
PpuMI 119 GGGTCCT [GA]G^G[AT]CC[CT]
590 AGGTCCT
Sau3AI 3 GATC ^GATC
729 GATC
Sau96I 120 GGTCC G^G[ACGT]CC
591 GGTCC
TfiI 262 GAATC G^A[AT]TC
319 GATTC
Tru9I 226 TTAA T^TAA
506 TTAA
TspRI 500 CAGTG CA[GC]TG[ACGT][ACGT]^
702 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AccII AciI BsiYI BslI BsmAI
BstF5I BstUI Cac8I Fnu4HI FokI
HapII HgaI HinfI HpaII ItaI
MaeII MaeIII MboII MnlI MspI
MvnI NlaIII SfaNI SmlI TaqI
Tsp45I Tsp509I TthHB8I