Restriction map for
pF1KA0228
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsaHI BseAI BsiWI BsmBI
BsmI BspDI BspEI BspLU11I BspMI
BssHII BssKI BssSI Bst1107I BstAPI
BstBI BstEII BstNI BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraII DraIII Eam1105I
Eco47III Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRII EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MamI MluI MroI MvaI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PaeR7I PflFI PflMI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScrFI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI Tth111I Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AflII 744 CTTAAG C^TTAAG
AgeI 1631 ACCGGT A^CCGGT
Alw44I 638 GTGCAC G^TGCAC
ApaLI 638 GTGCAC G^TGCAC
Asp700I 1378 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 106 CCCGAG C^[CT]CG[GA]G
AvaII 1744 GGACC G^G[AT]CC
BalI 597 TGGCCA TGG^CCA
BfrI 744 CTTAAG C^TTAAG
BsaAI 982 CACGTA [CT]AC^GT[GA]
BsaI 1555 GGTCTC GGTCTC(1/5)
BsaWI 1631 ACCGGT [AT]^CCGG[AT]
BseRI 1159 GAGGAG GAGGAG(10/8)
BsiEI 5 CGGCCG CG[GA][CT]^CG
BsiYI 1413 CCTTTCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1413 CCTTTCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 106 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1275 TGTACA T^GTACA
BsrDI 694 GCAATG GCAATG(2/0)
BsrFI 1631 ACCGGT [GA]^CCGG[CT]
BsrGI 1275 TGTACA T^GTACA
Cfr10I 1631 ACCGGT [GA]^CCGG[CT]
Cfr13I 1744 GGACC G^G[ACGT]CC
DraI 556 TTTAAA TTT^AAA
DrdI 152 GACGCTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 8 CCGTGG C^C[GA][CT]GG
EagI 5 CGGCCG C^GGCCG
EarI 1014 CTCTTC CTCTTC(1/4)
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
EcoT14I 1302 CCTTGG C^C[AT][AT]GG
FauI 83 CCCGC CCCGC(4/6)
HapII 1632 CCGG C^CGG
HgaI 152 GACGC GACGC(5/10)
HincII 161 GTCAAC GT[CT]^[GA]AC
HindII 161 GTCAAC GT[CT]^[GA]AC
HpaII 1632 CCGG C^CGG
Ksp632I 1014 CTCTTC CTCTTC(1/4)
MluNI 597 TGGCCA TGG^CCA
MscI 597 TGGCCA TGG^CCA
MspA1I 56 CCGCTG C[AC]G^C[GT]G
MspI 1632 CCGG C^CGG
NspI 810 ACATGC [GA]CATG^[CT]
PacI 802 TTAATTAA TTAAT^TAA
PinAI 1631 ACCGGT A^CCGGT
PleI 33 GACTC GAGTC(4/5)
PstI 338 CTGCAG CTGCA^G
Sau96I 1744 GGACC G^G[ACGT]CC
ScaI 204 AGTACT AGT^ACT
SmlI 744 CTTAAG C^T[CT][GA]AG
SspBI 1275 TGTACA T^GTACA
StyI 1302 CCTTGG C^C[AT][AT]GG
SwaI 555 ATTTAAAT ATTT^AAAT
XmnI 1378 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 332 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1198 CAGAGGCTG
AspHI 312 GTGCTC G[AT]GC[AT]^C
638 GTGCAC
BamHI 23 GGATCC G^GATCC
841 GGATCC
BbsI 1307 GAAGAC GAAGAC(2/6)
1233 GTCTTC
BmyI 312 GTGCTC G[AGT]GC[ACT]^C
638 GTGCAC
BpuAI 1307 GAAGAC GAAGAC(2/6)
1233 GTCTTC
BsaJI 8 CCGTGG C^C[ACGT][ACGT]GG
1302 CCTTGG
BsiHKAI 312 GTGCTC G[AT]GC[AT]^C
638 GTGCAC
Bsp1286I 312 GTGCTC G[AGT]GC[ACT]^C
638 GTGCAC
BspHI 996 TCATGA T^CATGA
1218 TCATGA
BsrBI 78 CCGCTC CCGCTC(-3/-3)
96 CCGCTC
BstYI 23 GGATCC [GA]^GATC[CT]
841 GGATCC
EaeI 5 CGGCCG [CT]^GGCC[GA]
597 TGGCCA
MaeII 777 ACGT A^CGT
983 ACGT
MaeIII 659 GTCAC ^GT[ACGT]AC
1657 GTGAC
MfeI 1169 CAATTG C^AATTG
1499 CAATTG
MflI 23 GGATCC [GA]^GATC[CT]
841 GGATCC
MunI 1169 CAATTG C^AATTG
1499 CAATTG
RcaI 996 TCATGA T^CATGA
1218 TCATGA
SspI 857 AATATT AAT^ATT
1649 AATATT
TfiI 328 GAATC G^A[AT]TC
1455 GAATC
Tsp45I 659 GTCAC ^GT[GC]AC
1657 GTGAC
XcmI 1029 CCAGTATAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1697 CCACTGAAGAAGTGG
XhoII 23 GGATCC [GA]^GATC[CT]
841 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BfaI BsgI BsmAI
BsmFI BsrI BstF5I Cac8I DdeI
DpnII Fnu4HI FokI HaeIII HindIII
HinfI HphI ItaI MaeI MboI
MboII MnlI MseI MslI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
SfaNI SfcI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I